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dc.contributor.author | Martí, Maricarmen | es_ES |
dc.contributor.author | Diretto, Gianfranco | es_ES |
dc.contributor.author | Aragones, V | es_ES |
dc.contributor.author | Frusciante, Sarah | es_ES |
dc.contributor.author | Ahrazem, Oussama | es_ES |
dc.contributor.author | Gómez-Gómez, Lourdes | es_ES |
dc.contributor.author | Daròs, José-Antonio | es_ES |
dc.date.accessioned | 2021-09-14T03:33:09Z | |
dc.date.available | 2021-09-14T03:33:09Z | |
dc.date.issued | 2020-09 | es_ES |
dc.identifier.issn | 1096-7176 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/172300 | |
dc.description.abstract | [EN] Crocins and picrocrocin are glycosylated apocarotenoids responsible, respectively, for the color and the unique taste of the saffron spice, known as red gold due to its high price. Several studies have also shown the health-promoting properties of these compounds. However, their high costs hamper the wide use of these metabolites in the pharmaceutical sector. We have developed a virus-driven system to produce remarkable amounts of crocins and picrocrocin in adult Nicotiana benthamiana plants in only two weeks. The system consists of viral clones derived from tobacco etch potyvirus that express specific carotenoid cleavage dioxygenase (CCD) enzymes from Crocus sativus and Buddleja davidii. Metabolic analyses of infected tissues demonstrated that the sole virus driven expression of C. sativus CsCCD2L or B. davidii BdCCD4.1 resulted in the production of crocins, picrocrocin and safranal. Using the recombinant virus that expressed CsCCD2L, accumulations of 0.2% of crocins and 0.8% of picrocrocin in leaf dry weight were reached in only two weeks. In an attempt to improve apocarotenoid content in N. benthamiana, co-expression of CsCCD2L with other carotenogenic enzymes, such as Pantoea ananatis phytoene synthase (PaCrtB) and saffron beta-carotene hydroxylase 2 (BCH2), was performed using the same viral system. This combinatorial approach led to an additional crocin increase up to 0.35% in leaves in which CsCCD2L and PaCrtB were co-expressed. Considering that saffron apocarotenoids are costly harvested from flower stigma once a year, and that Buddleja spp. flowers accumulate lower amounts, this system may be an attractive alternative for the sustainable production of these appreciated metabolites. | es_ES |
dc.description.sponsorship | We thank K. Schreiber and C. Mares (IBMCP, CSIC-UPV, Valencia, Spain) for technical assistance during plant transformation. We thank M. Gasc.on and M.D. G.omez-Jim.enez (IBMCP, CSIC-UPV, Valencia, Spain) for helpful assistance with LSCM analyses. We thank D. Dubbala (IBMCP, CSIC-UPV, Valencia, Spain) for English revision. This work was supported by grants BIO2016-77000-R and BIO2017-83184-R from the Spanish Ministerio de Ciencia e Innovacion (co-financed European Union ERDF), and SBPLY/17/180501/000234 from Junta de Comunidades de Castilla-La Mancha. M.M. was the recipient of a predoctoral fellowship from the Spanish Ministerio de Educacion, Cultura y Deporte (FPU16/05294). G.D. and L.G.G. are participants of the European COST action CA15136 (EUROCAROTEN). L.G.G. is a participant of the CARNET network (BIO2015-71703-REDT and BIO2017-90877-RED). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | nfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83184-R/ES/VIRUS DE PLANTAS: PATOGENOS Y TAMBIEN VECTORES PARA LA PRODUCCION DE PROTEINAS, METABOLITOS, RNAS Y NANOPARTICULAS/ | es_ES |
dc.relation.ispartof | Metabolic Engineering | es_ES |
dc.rights | Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) | es_ES |
dc.subject | Apocarotenoids | es_ES |
dc.subject | Crocins | es_ES |
dc.subject | Picrocrocin | es_ES |
dc.subject | Carotenoid cleavage dioxygenase | es_ES |
dc.subject | Viral vector | es_ES |
dc.subject | Tobacco etch virus | es_ES |
dc.subject | Potyvirus | es_ES |
dc.subject.classification | GENETICA | es_ES |
dc.title | Efficient production of saffron crocins and picrocrocin in Nicotiana benthamiana using a virus-driven system | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.ymben.2020.06.009 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/JCCM//SBPLY%2F17%2F180501%2F000234/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BIO2015-71703-REDT/ES/CAROTENOIDES EN RED: DE LOS MICROORGANISMOS Y LAS PLANTAS A LOS ALIMENTOS Y LA SALUD/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MECD//FPU14%2F05294/ES/FPU14%2F05294/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/COST//CA15136/EU/European network to advance carotenoid research and applications in agro-food and health (EUROCAROTEN)/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI//BIO2017-90877-REDT/ES/CARED: RED ESPAÑOLA DE CAROTENOIDES/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BIO2016-77000-R/ES/ELUCIDACION DEL MAPA DE LOS APOCAROTENOIDES DURANTE EL DESARROLLO DEL AZAFRAN: DESDE PIGMENTOS A REGULADORES/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.description.bibliographicCitation | Martí, M.; Diretto, G.; Aragones, V.; Frusciante, S.; Ahrazem, O.; Gómez-Gómez, L.; Daròs, J. (2020). Efficient production of saffron crocins and picrocrocin in Nicotiana benthamiana using a virus-driven system. Metabolic Engineering. 61:238-250. https://doi.org/10.1016/j.ymben.2020.06.009 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.ymben.2020.06.009 | es_ES |
dc.description.upvformatpinicio | 238 | es_ES |
dc.description.upvformatpfin | 250 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 61 | es_ES |
dc.identifier.pmid | 32629020 | es_ES |
dc.relation.pasarela | S\425113 | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |
dc.contributor.funder | Junta de Comunidades de Castilla-La Mancha | es_ES |
dc.contributor.funder | Ministerio de Educación, Cultura y Deporte | es_ES |
dc.contributor.funder | European Cooperation in Science and Technology | es_ES |
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