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Efficient evaluation of a gene containment system for poplar through early flowering induction

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Efficient evaluation of a gene containment system for poplar through early flowering induction

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dc.contributor.author Briones, M. Valentina es_ES
dc.contributor.author Hoenicka, Hans es_ES
dc.contributor.author Cañas Clemente, Luís Antonio es_ES
dc.contributor.author BELTRAN PORTER, JOSE PIO es_ES
dc.contributor.author Hanelt, Dieter es_ES
dc.contributor.author Sharry, Sandra es_ES
dc.contributor.author Fladung, Matthias es_ES
dc.date.accessioned 2021-11-05T12:36:53Z
dc.date.available 2021-11-05T12:36:53Z
dc.date.issued 2020-05 es_ES
dc.identifier.issn 0721-7714 es_ES
dc.identifier.uri http://hdl.handle.net/10251/176109
dc.description.abstract [EN] Key message The early flowering system HSP::AtFT allowed a fast evaluation of a gene containment system based on the construct PsEND1::barnase-barstar for poplar. Transgenic lines showed disturbed pollen development and sterility. Vertical gene transfer through pollen flow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene flow among tree species. However, evaluation of genetic containment strategies for trees is very difficult due to the long-generation times. Early flowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile flowers in poplar, recently, a new early flowering approach was developed. In this study, early flowering poplar lines containing the gene construct PsEND1::barnase-barstar were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efficiency for generation of male-sterile plants fused to the barnase gene. RT-PCRs confirmed barnase gene activity in flowers, and pollen development was disturbed, leading to sterile flowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species. es_ES
dc.description.sponsorship Open Access funding provided by Projekt DEAL. This work was funded with a scholarship by the Deutscher Akademischer Austauschdienst (DAAD). We thank S. Bein, D. Ebbinghaus, and A. Worm for helpful technical assistance in the laboratory, and the greenhouse staff (M. Hunger, G. Wiemann, R. Ebbinghaus, and M. Spauszus) for plant cultivation. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Plant Cell Reports es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Gene containment es_ES
dc.subject Early fowering es_ES
dc.subject Genetic transformation es_ES
dc.subject Populus es_ES
dc.subject PsEND1 es_ES
dc.subject Biosafety es_ES
dc.title Efficient evaluation of a gene containment system for poplar through early flowering induction es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00299-020-02515-1 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Briones, MV.; Hoenicka, H.; Cañas Clemente, LA.; Beltran Porter, JP.; Hanelt, D.; Sharry, S.; Fladung, M. (2020). Efficient evaluation of a gene containment system for poplar through early flowering induction. Plant Cell Reports. 39(5):577-587. https://doi.org/10.1007/s00299-020-02515-1 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s00299-020-02515-1 es_ES
dc.description.upvformatpinicio 577 es_ES
dc.description.upvformatpfin 587 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 39 es_ES
dc.description.issue 5 es_ES
dc.identifier.pmid 32052127 es_ES
dc.identifier.pmcid PMC7165154 es_ES
dc.relation.pasarela S\433270 es_ES
dc.contributor.funder Projekt DEAL es_ES
dc.contributor.funder Deutscher Akademischer Austauschdienst es_ES
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