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Albino T-DNA tomato mutant reveals a key function of 1-deoxy-D-xylulose-5-phosphate synthase (DXS1) in plant development and survival

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Albino T-DNA tomato mutant reveals a key function of 1-deoxy-D-xylulose-5-phosphate synthase (DXS1) in plant development and survival

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dc.contributor.author Garcia-Alcazar, Manuel es_ES
dc.contributor.author Giménez Caminero, Maria Estela es_ES
dc.contributor.author Pineda Chaza, Benito José es_ES
dc.contributor.author Capel, Carmen es_ES
dc.contributor.author García Sogo, Begoña es_ES
dc.contributor.author Sánchez Martín-Sauceda, Sibilla es_ES
dc.contributor.author Yuste-Lisbona, Fernando J. es_ES
dc.contributor.author Angosto, Trinidad es_ES
dc.contributor.author Capel, Juan es_ES
dc.contributor.author Moreno Ferrero, Vicente es_ES
dc.contributor.author Lozano, Rafael es_ES
dc.date.accessioned 2022-05-10T18:06:14Z
dc.date.available 2022-05-10T18:06:14Z
dc.date.issued 2017-03-28 es_ES
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://hdl.handle.net/10251/182474
dc.description.abstract [EN] Photosynthetic activity is indispensable for plant growth and survival and it depends on the synthesis of plastidial isoprenoids as chlorophylls and carotenoids. In the non-mevalonate pathway (MEP), the 1-deoxy-D-xylulose-5-phosphate synthase 1 (DXS1) enzyme has been postulated to catalyze the ratelimiting step in the formation of plastidial isoprenoids. In tomato, the function of DXS1 has only been studied in fruits, and hence its functional relevance during plant development remains unknown. Here we report the characterization of the wls-2297 tomato mutant, whose severe deficiency in chlorophylls and carotenoids promotes an albino phenotype. Additionally, growth of mutant seedlings was arrested without developing vegetative organs, which resulted in premature lethality. Gene cloning and silencing experiments revealed that the phenotype of wls-2297 mutant was caused by 38.6 kb-deletion promoted by a single T-DNA insertion affecting the DXS1 gene. This was corroborated by in vivo and molecular complementation assays, which allowed the rescue of mutant phenotype. Further characterization of tomato plants overexpressing DXS1 and comparative expression analysis indicate that DXS1 may play other important roles besides to that proposed during fruit carotenoid biosynthesis. Taken together, these results demonstrate that DXS1 is essentially required for the development and survival of tomato plants. es_ES
dc.description.sponsorship This work was supported by research grants from the Spanish Ministry of Economy and Competitiveness and the UE-European Regional Development Fund (AGL2015-64991-C3-1-R, and AGL2015-64991-C3-3-R), and Junta de Andalucia (P12-AGR-1482). PhD fellowship to M.G.-A. was funded by the FPU Programme of the Spanish Ministry of Science and Innovation. The authors thank research facilities provided by the Campus de Excelencia Internacional Agroalimentario (CeiA3). es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject.classification GENETICA es_ES
dc.title Albino T-DNA tomato mutant reveals a key function of 1-deoxy-D-xylulose-5-phosphate synthase (DXS1) in plant development and survival es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/srep45333 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2015-64991-C3-1-R/ES/GENOMICA FUNCIONAL Y MEJORA GENETICA DE LA PRODUCTIVIDAD DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO-ESTRES ABIOTICO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Junta de Andalucía//P12-AGR-1482/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2015-64991-C3-3-R/ES/GENOMICA FUNCIONAL Y MEJORA GENETICA DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO - ESTRES ABIOTICO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Garcia-Alcazar, M.; Giménez Caminero, ME.; Pineda Chaza, BJ.; Capel, C.; García Sogo, B.; Sánchez Martín-Sauceda, S.; Yuste-Lisbona, FJ.... (2017). Albino T-DNA tomato mutant reveals a key function of 1-deoxy-D-xylulose-5-phosphate synthase (DXS1) in plant development and survival. Scientific Reports. 7:1-12. https://doi.org/10.1038/srep45333 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/srep45333 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 12 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.identifier.pmid 28350010 es_ES
dc.identifier.pmcid PMC5368609 es_ES
dc.relation.pasarela S\352889 es_ES
dc.contributor.funder Junta de Andalucía es_ES
dc.contributor.funder MINISTERIO DE ECONOMIA Y EMPRESA es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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