Resumen:
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[EN] The development of high nitrogen use efficiency (NUE) cultivars under low N inputs is required for sustainable agriculture. To this end, in this study, we analyzed the impact of long-term suboptimal N conditions on ...[+]
[EN] The development of high nitrogen use efficiency (NUE) cultivars under low N inputs is required for sustainable agriculture. To this end, in this study, we analyzed the impact of long-term suboptimal N conditions on the metabolome and transcriptome of tomato to identify specific molecular processes and regulators at the organ scale. Physiological and metabolic analysis revealed specific responses to maintain glutamate, asparagine, and sucrose synthesis in leaves for partition to sustain growth, while assimilated C surplus is stored in the roots. The transcriptomic analyses allowed us to identify root and leaf sets of genes whose expression depends on N availability. GO analyses of the identified genes revealed conserved biological functions involved in C and N metabolism and remobilization as well as other specifics such as the mitochondrial alternative respiration and chloroplastic cyclic electron flux. In addition, integrative analyses uncovered N regulated genes in root and leaf clusters, which are positively correlated with changes in the levels of different metabolites such as organic acids, amino acids, and formate. Interestingly, we identified transcription factors with high identity to TGA4, ARF8, HAT22, NF-YA5, and NLP9, which play key roles in N responses in Arabidopsis. Together, this study provides a set of nitrogen-responsive genes in tomato and new putative targets for tomato NUE and fruit quality improvement under limited N supply.
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Código del Proyecto:
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info:eu-repo/grantAgreement/MCIU//SEV-2016-0672/
...[+]
info:eu-repo/grantAgreement/MCIU//SEV-2016-0672/
info:eu-repo/grantAgreement/INIA//RTA2015-00014-c02-01/
info:eu-repo/grantAgreement/CAM//S2018%2FBAA-4330//AGRISOST-CM/
info:eu-repo/grantAgreement/FONDECYT//1190812/
info:eu-repo/grantAgreement/ANID//ICN17-022//Millennium Science Initiative Program/
info:eu-repo/grantAgreement/AGENCIA ESTATAL DE INVESTIGACION//RTA2015-00014-C02-02//CARACTERIZACION AGRONOMICA, FISIOLOGICA Y MOLECULAR DE GENOTIPOS DE TOMATE CON MAYOR EFICIENCIA EN LA SINTESIS DE ASIMILADOS Y EN EL USO DE NITROGENO/
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Agradecimientos:
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This study was supported by grants from The National Institute for Agriculture and Food Research and Technology (CSIC-INIA) (RTA2015-00014-c02-00 to JMA and RTA2015-00014-c02-01 to SGN) and the Community of Madrid (AGRISOST-CM ...[+]
This study was supported by grants from The National Institute for Agriculture and Food Research and Technology (CSIC-INIA) (RTA2015-00014-c02-00 to JMA and RTA2015-00014-c02-01 to SGN) and the Community of Madrid (AGRISOST-CM S2018/BAA-4330 to JMA). We also want to acknowledge the "Severo Ochoa Program for Centers of Excellence in R&D" from the Agencia Estatal de Investigacion of Spain (Grant SEV-2016-0672) for supporting the scientific services used in this study. J. Canales was supported by the Agencia Nacional de Investigacion y Desarrollo de Chile (ANID, FONDECYT 1190812) and ANID-Millennium Science Initiative Program (ICN17-022).
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