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Integrative Transcriptomic and Metabolomic Analysis at Organ Scale Reveals Gene Modules Involved in the Responses to Suboptimal Nitrogen Supply in Tomato

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Integrative Transcriptomic and Metabolomic Analysis at Organ Scale Reveals Gene Modules Involved in the Responses to Suboptimal Nitrogen Supply in Tomato

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dc.contributor.author Renau-Morata, Begoña es_ES
dc.contributor.author Molina Romero, Rosa Victoria es_ES
dc.contributor.author Minguet, E.G. es_ES
dc.contributor.author Cebolla Cornejo, Jaime es_ES
dc.contributor.author Carrillo, Laura es_ES
dc.contributor.author Martí-Renau, Raul es_ES
dc.contributor.author García-Carpintero, Víctor es_ES
dc.contributor.author Jiménez-Benavente, Eva es_ES
dc.contributor.author Yang, Lu es_ES
dc.contributor.author Cañizares Sales, Joaquín es_ES
dc.contributor.author Canales, Javier es_ES
dc.contributor.author Medina, Joaquín es_ES
dc.contributor.author Nebauer, Sergio G. es_ES
dc.date.accessioned 2022-05-30T18:05:02Z
dc.date.available 2022-05-30T18:05:02Z
dc.date.issued 2021-07 es_ES
dc.identifier.uri http://hdl.handle.net/10251/182989
dc.description.abstract [EN] The development of high nitrogen use efficiency (NUE) cultivars under low N inputs is required for sustainable agriculture. To this end, in this study, we analyzed the impact of long-term suboptimal N conditions on the metabolome and transcriptome of tomato to identify specific molecular processes and regulators at the organ scale. Physiological and metabolic analysis revealed specific responses to maintain glutamate, asparagine, and sucrose synthesis in leaves for partition to sustain growth, while assimilated C surplus is stored in the roots. The transcriptomic analyses allowed us to identify root and leaf sets of genes whose expression depends on N availability. GO analyses of the identified genes revealed conserved biological functions involved in C and N metabolism and remobilization as well as other specifics such as the mitochondrial alternative respiration and chloroplastic cyclic electron flux. In addition, integrative analyses uncovered N regulated genes in root and leaf clusters, which are positively correlated with changes in the levels of different metabolites such as organic acids, amino acids, and formate. Interestingly, we identified transcription factors with high identity to TGA4, ARF8, HAT22, NF-YA5, and NLP9, which play key roles in N responses in Arabidopsis. Together, this study provides a set of nitrogen-responsive genes in tomato and new putative targets for tomato NUE and fruit quality improvement under limited N supply. es_ES
dc.description.sponsorship This study was supported by grants from The National Institute for Agriculture and Food Research and Technology (CSIC-INIA) (RTA2015-00014-c02-00 to JMA and RTA2015-00014-c02-01 to SGN) and the Community of Madrid (AGRISOST-CM S2018/BAA-4330 to JMA). We also want to acknowledge the "Severo Ochoa Program for Centers of Excellence in R&D" from the Agencia Estatal de Investigacion of Spain (Grant SEV-2016-0672) for supporting the scientific services used in this study. J. Canales was supported by the Agencia Nacional de Investigacion y Desarrollo de Chile (ANID, FONDECYT 1190812) and ANID-Millennium Science Initiative Program (ICN17-022). es_ES
dc.language Inglés es_ES
dc.publisher MDPI es_ES
dc.relation.ispartof Agronomy es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Nitrogen es_ES
dc.subject Suboptimal conditions es_ES
dc.subject Metabolome es_ES
dc.subject Transcriptome es_ES
dc.subject Growth es_ES
dc.subject Leaves es_ES
dc.subject Roots es_ES
dc.subject Solanum lycopersicum es_ES
dc.subject Tomato es_ES
dc.subject.classification GENETICA es_ES
dc.subject.classification FISIOLOGIA VEGETAL es_ES
dc.title Integrative Transcriptomic and Metabolomic Analysis at Organ Scale Reveals Gene Modules Involved in the Responses to Suboptimal Nitrogen Supply in Tomato es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/agronomy11071320 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MCIU//SEV-2016-0672/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/INIA//RTA2015-00014-c02-01/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CAM//S2018%2FBAA-4330//AGRISOST-CM/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FONDECYT//1190812/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ANID//ICN17-022//Millennium Science Initiative Program/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AGENCIA ESTATAL DE INVESTIGACION//RTA2015-00014-C02-02//CARACTERIZACION AGRONOMICA, FISIOLOGICA Y MOLECULAR DE GENOTIPOS DE TOMATE CON MAYOR EFICIENCIA EN LA SINTESIS DE ASIMILADOS Y EN EL USO DE NITROGENO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Producción Vegetal - Departament de Producció Vegetal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Renau-Morata, B.; Molina Romero, RV.; Minguet, E.; Cebolla Cornejo, J.; Carrillo, L.; Martí-Renau, R.; García-Carpintero, V.... (2021). Integrative Transcriptomic and Metabolomic Analysis at Organ Scale Reveals Gene Modules Involved in the Responses to Suboptimal Nitrogen Supply in Tomato. Agronomy. 11(7):1-26. https://doi.org/10.3390/agronomy11071320 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/agronomy11071320 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 26 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 11 es_ES
dc.description.issue 7 es_ES
dc.identifier.eissn 2073-4395 es_ES
dc.relation.pasarela S\441504 es_ES
dc.contributor.funder Comunidad de Madrid es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder Ministerio de Ciencia, Innovación y Universidades es_ES
dc.contributor.funder Agencia Nacional de Investigación y Desarrollo de Chile es_ES
dc.contributor.funder Fondo Nacional de Desarrollo Científico y Tecnológico, Chile es_ES
dc.contributor.funder Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria es_ES
dc.subject.ods 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible es_ES
upv.costeAPC 589,19 es_ES


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