Preparation of monolithic polymer-magnetite nanoparticle composites into poly(ethylene-co-tetrafluoroethylene) tubes for uses in micro-bore HPLC separation and extraction of phosphorylated compounds
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Preparation of monolithic polymer-magnetite nanoparticle composites into poly(ethylene-co-tetrafluoroethylene) tubes for uses in micro-bore HPLC separation and extraction of phosphorylated compounds
Torres-Cartas, S.; Meseguer-Lloret, S.; Gómez Benito, C.; Catalá-Icardo, M.; Simo-Alfonso, EF.; Herrero-Martínez, JM. (2021). Preparation of monolithic polymer-magnetite nanoparticle composites into poly(ethylene-co-tetrafluoroethylene) tubes for uses in micro-bore HPLC separation and extraction of phosphorylated compounds. Talanta. 224:121806-121813. https://doi.org/10.1016/j.talanta.2020.121806
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/183098
Preparation of monolithic polymer-magnetite nanoparticle composites into poly(ethylene-co-tetrafluoroethylene) tubes for uses in micro-bore HPLC separation and extraction of phosphorylated compounds
[EN] This paper describes the fabrication of a novel microbore monolithic column modified with magnetite nanoparticles (MNPs) prepared in a poly(ethylene-co-tetrafluoroethylene) (EFTE) tubing, and its application as ...[+]
[EN] This paper describes the fabrication of a novel microbore monolithic column modified with magnetite nanoparticles (MNPs) prepared in a poly(ethylene-co-tetrafluoroethylene) (EFTE) tubing, and its application as stationary phase for the chromatographic separation of phosphorylated compounds. In order to obtain the composite column, a two-step procedure was performed. The formation of a glycidyl methacrylate-based monolith inside the activated ETFE tube was firstly carried out. Then, two incorporation approaches of MNPs in monoliths were investigated. The generic polymer was modified with 3-aminopropyltrimethoxysilane (APTMS) to be subsequently attached to MNP surfaces. Alternatively, APTMS-coated MNPs were firstly prepared and subsequently used for attachment onto the monolith surface through reaction of epoxy groups present in the generic monolith. This last strategy gave a reproducible layer of MNPs coated onto the polymer monolith as well as robust and permeable chromatographic columns. The retention behaviour of this MNP-based composite monolithic column was studied by using small phosphorylated compounds (adenosine phosphates). It was found that the retention of model analytes was ruled by partitioning and adsorption HILIC mechanisms. The columns also exhibited satisfactory performance in the separation of these target compounds, showing good chromatographic behaviour after two months of continued use. These composite monolithic columns were also successfully applied to the extraction of a tryptic digest of beta-casein.[-]
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095536-B-I00/ES/DESARROLLO DE MATERIALES POLIMERICOS FUNCIONALES EN PLATAFORMAS FLEXIBLES PARA APLICACIONES MEDIOAMBIENTALES Y TOXICOLOGICAS/ info:eu-repo/grantAgreement/UV//PT13%2F0001/
Thanks:
This work was supported by project RTI2018-095536-B-I00 (Ministry of Science, Innovation and Universities, Spain). The proteomic analysis was performed in the proteomics facility of SCSIE (University of Valencia) that ...[+]
This work was supported by project RTI2018-095536-B-I00 (Ministry of Science, Innovation and Universities, Spain). The proteomic analysis was performed in the proteomics facility of SCSIE (University of Valencia) that belongs to ProteoRed, PRB2-ISCIII, supported by grant PT13/0001 (Instituto de Carlos III, Spain). The authors also thank Dr. Luz Valero-Rustarazo for her assistance in proteomic analysis and data treatment[-]