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dc.contributor.author | Puchalt-Rodríguez, Joan Carles | es_ES |
dc.contributor.author | Sánchez Salmerón, Antonio José | es_ES |
dc.contributor.author | Ivorra, Eugenio | es_ES |
dc.contributor.author | Llopis, Silvia | es_ES |
dc.contributor.author | Martínez, Roberto | es_ES |
dc.contributor.author | Martorell, Patricia | es_ES |
dc.date.accessioned | 2022-06-27T18:06:54Z | |
dc.date.available | 2022-06-27T18:06:54Z | |
dc.date.issued | 2021-06-10 | es_ES |
dc.identifier.issn | 2045-2322 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/183627 | |
dc.description.abstract | [EN] Traditionally Caenorhabditis elegans lifespan assays are performed by manually inspecting nematodes with a dissection microscope, which involves daily counting of live/dead worms cultured in Petri plates for 21-25 days. This manual inspection requires the screening of hundreds of worms to ensure statistical robustness, and is therefore a time-consuming approach. In recent years, various automated artificial vision systems have been reported to increase the throughput, however they usually provide less accurate results than manual assays. The main problems identified when using these vision systems are the false positives and false negatives, which occur due to culture media changes, occluded zones, dirtiness or condensation of the Petri plates. In this work, we developed and described a new C. elegans monitoring machine, SiViS, which consists of a flexible and compact platform design to analyse C. elegans cultures using the standard Petri plates seeded with E. coli. Our system uses an active vision illumination technique and different image-processing pipelines for motion detection, both previously reported, providing a fully automated image processing pipeline. In addition, this study validated both these methods and the feasibility of the SiViS machine for lifespan experiments by comparing them with manual lifespan assays. Results demonstrated that the automated system yields consistent replicates (p-value log rank test 0.699), and there are no significant differences between automated system assays and traditionally manual assays (p-value 0.637). Finally, although we have focused on the use of SiViS in longevity assays, the system configuration is flexible and can, thus, be adapted to other C. elegans studies such as toxicity, mobility and behaviour. | es_ES |
dc.description.sponsorship | This study was also supported by CDTI agency of the Spanish Ministry of Economy and Competitiveness under the CIEN project SMARTFOODS, Universitat Politecnica de Valencia under the project 20170020-UPV, Plan Nacional de I+D under the project RTI2018-094312-B-I00 and by the European FEDER funds. ADM Nutrition, Biopolis SL and Archer Daniels Midland provided support in the form of salaries for authors S. Llopis, R. Martinez, and P. Martorell. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.relation.ispartof | Scientific Reports | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject.classification | INGENIERIA DE SISTEMAS Y AUTOMATICA | es_ES |
dc.title | Small flexible automated system for monitoring Caenorhabditis elegans lifespan based on active vision and image processing techniques | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1038/s41598-021-91898-6 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094312-B-I00/ES/MONITORIZACION AVANZADA DE COMPORTAMIENTOS DE CAENORHABDITIS ELEGANS, BASADA EN VISION ACTIVA, PARA ANALIZAR FUNCION COGNITIVA Y ENVEJECIMIENTO/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UPV//20170020-UPV/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Ingeniería de Sistemas y Automática - Departament d'Enginyeria de Sistemes i Automàtica | es_ES |
dc.description.bibliographicCitation | Puchalt-Rodríguez, JC.; Sánchez Salmerón, AJ.; Ivorra, E.; Llopis, S.; Martínez, R.; Martorell, P. (2021). Small flexible automated system for monitoring Caenorhabditis elegans lifespan based on active vision and image processing techniques. Scientific Reports. 11(1):1-11. https://doi.org/10.1038/s41598-021-91898-6 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1038/s41598-021-91898-6 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 11 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 11 | es_ES |
dc.description.issue | 1 | es_ES |
dc.identifier.pmid | 34112931 | es_ES |
dc.identifier.pmcid | PMC8192789 | es_ES |
dc.relation.pasarela | S\439994 | es_ES |
dc.contributor.funder | AGENCIA ESTATAL DE INVESTIGACION | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Universitat Politècnica de València | es_ES |
dc.contributor.funder | Centro para el Desarrollo Tecnológico Industrial | es_ES |
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dc.subject.ods | 03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades | es_ES |