- -

Influence of alpha linolenic acid on the motility, viability, antioxidant activity and fertility of frozen-thawed New Zealand white rabbit buck semen

RiuNet: Repositorio Institucional de la Universidad Politécnica de Valencia

Compartir/Enviar a

Citas

Estadísticas

  • Estadisticas de Uso

Influence of alpha linolenic acid on the motility, viability, antioxidant activity and fertility of frozen-thawed New Zealand white rabbit buck semen

Mostrar el registro sencillo del ítem

Ficheros en el ítem

Thumbnail
Nombre: El-ShahatFadlAbdelnaby ...
Tamaño: 300.4Kb
Formato: PDF
Descripción: Versión editorial
Abrir
dc.contributor.author El-Shahat, Khaled Hafez es_ES
dc.contributor.author Fadl, Aya Mohamed es_ES
dc.contributor.author Abdelnaby, Elshymaa Ahmed es_ES
dc.date.accessioned 2022-10-10T07:18:09Z
dc.date.available 2022-10-10T07:18:09Z
dc.date.issued 2022-09-30
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/187318
dc.description.abstract [EN] Freezing and thawing processes result in production and accumulation of high concentrations of reactive oxygen species that are detrimental to spermatozoal motility and fertility. Therefore, supplementation of exogenous source of antioxidants to freezing diluent is crucial. The aim of the present study was to investigate for the first time whether supplementation of semen diluent with alpha linolenic acid (ALA) can improve motility, viability, membrane integrity, antioxidant status and fertility of post-thaw rabbit spermatozoa. Semen was collected and pooled from fifteen New Zealand white rabbit bucks. Semen samples were diluted with a tris-citrate-glucose (TCG) extender supplemented with ALA (0, 50, 75 and 100 μmol). Then, extended rabbit semen was cooled at 5°C and cryopreserved in liquid nitrogen. After thawing, spermatozoal quality parameters (individual motility %, viability %, osmotic resistance %, and acrosome integrity %), antioxidant activity (SOD, CAT, and GSH activities), lipid peroxidation (malondialdehyde) and fertility (conception and kindling rates) were evaluated. Results revealed that supplementation of rabbit semen extender with 50 μmol ALA significantly (P<0.05) increased spermatozoal characteristics including motility (56.54%), viability (60.01%), acrosome status (72.66%) and membrane integrity (59.13%). The activity of semen antioxidant enzymes (SOD, CAT, and GSH) showed a significant improvement with a marked decrease in lipid peroxidation. Moreover, the conception (73.30%) and kindling (70.00%) rates were significantly (P<0.05) higher in does inseminated with thawed semen treated with 50 μmol ALA in comparison with other concentrations (0, 75 and 100 μmol). In summary, supplementation of rabbit semen extender with 50 μmol ALA improved motility, viability, membrane integrity, acrosome integrity, antioxidant enzymes activity and fertility of post-thaw rabbit spermatozoa. Our findings suggested that higher concentrations of ALA are detrimental to post-thaw characteristics of New Zealand white rabbit buck spermatozoa. To achieve better results, the semen freezing extender should be supplemented with ALA at lower concentrations, especially 50 μmol. es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.relation.ispartof World Rabbit Science es_ES
dc.rights Reconocimiento - No comercial - Compartir igual (by-nc-sa) es_ES
dc.subject Rabbit semen es_ES
dc.subject Cryopreservation es_ES
dc.subject Alpha linolenic acid es_ES
dc.subject Antioxidant es_ES
dc.subject Fertility es_ES
dc.title Influence of alpha linolenic acid on the motility, viability, antioxidant activity and fertility of frozen-thawed New Zealand white rabbit buck semen es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.4995/wrs.2022.17042
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation El-Shahat, KH.; Fadl, AM.; Abdelnaby, EA. (2022). Influence of alpha linolenic acid on the motility, viability, antioxidant activity and fertility of frozen-thawed New Zealand white rabbit buck semen. World Rabbit Science. 30(3):219-226. https://doi.org/10.4995/wrs.2022.17042 es_ES
dc.description.accrualMethod OJS es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2022.17042 es_ES
dc.description.upvformatpinicio 219 es_ES
dc.description.upvformatpfin 226 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 30 es_ES
dc.description.issue 3 es_ES
dc.identifier.eissn 1989-8886
dc.relation.pasarela OJS\17042 es_ES
dc.description.references Abavisani A., Arshami J., Naserian A.A., Sheikholeslami Kandelousi M.A., Azizzadeh M. 2013. Quality of bovine chilled or frozen-thawed semen after addition of omega-3 fatty acids supplementation to extender. Int. J. Fertil. Steril., 7: 161-168. es_ES
dc.description.references Abdi-Benemar H., Jafaroghli M., Khalili B., Zamiri M.J., Ezazi H., Shadparvar A.A. 2015. Effects of DHA supplementation of the extender containing egg yolk and alpha tocopherol on the freezability and post-thawing fertility of ram semen. Small Rumin. Res., 130: 166-170. es_ES
dc.description.references https://doi.org/10.1016/j.smallrumres.2015.06.013 es_ES
dc.description.references Aebi H. 1984. Catalase in vitro. Methods Enzymol., 105: 121-126. https://doi.org/10.1016/S0076-6879(84)05016-3 es_ES
dc.description.references Aksoy Y., Aksoy H., Altinkaynak K., Aydin H.R., Ozkan A. 2006. Sperm fatty acid composition in subfertile men. Prostaglandins Leukot. Essent. Fatty Acids., 75: 75-79. https://doi.org/10.1016/j.plefa.2006.06.002 es_ES
dc.description.references Alvarez J.G., Touchstone J.C., Blasco L., Storey B.T. 1987. Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity. Andrologia, 8: 338-345. es_ES
dc.description.references https://doi.org/10.1002/j.1939-4640.1987.tb00973.x es_ES
dc.description.references Ansari M., Towhidi A., Shahrbabak M., Bahreini M. 2012. Docosahexaenoic acid and alpha-tocopherol improve sperm cryosurvival in goat. Slovak J. Anim., 45: 7-13. es_ES
dc.description.references Bailey J.L., Bilodeau J., Cormier N. 2000. Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon. Andrologia, 21: 47-54. es_ES
dc.description.references Bucak M.N., Atessahin A., Varisli O., Yuce A., Tekin N., Akcay A. 2007. The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen microscopic and oxidative stress parameters after freezethawing process. Theriogenology, 67: 1060-1067. https://doi.org/10.1016/j.theriogenology.2006.12.004 es_ES
dc.description.references Castellano C.A., Audet I., Bailey J., Laforest P., Matte J. 2010. Dietary omega-3 fatty acids (fish oils) have limited effects on boar semen stored at 17°C or cryopreserved. Theriogenology, 74: 1482-1490. https://doi.org/10.1016/j.theriogenology.2010.06.020 es_ES
dc.description.references Castellini C., Lattaioli P., Dal Bosco A., Minelli A., Mugnai C. 2003. Oxidative status and semen characteristics of rabbit buck as es_ES
dc.description.references affected by dietary vitamin E, C and n-3 fatty acids. Reprod. Nutr. Dev., 43: 91-103. https://doi.org/10.1051/rnd:2003008 es_ES
dc.description.references Castellini C., Dal Bosco A., Cardinali R. Mugnai C. 2004. Effect of dietary α-linolenic acid on semen characteristics of rabbit bucks. In Proc.: 8th World Rabbit Congress, September, Puebla, Mexico. 245-250. es_ES
dc.description.references Castellini C., Mattioli S., Signorini C., Cotozzolo E., Noto D., Moretti E., Brecchia G., Dal Bosco A., Belmonte G., Durand T., De Felice C., Collodel G. 2019. Effect of Dietary n-3 Source on Rabbit Male Reproduction. Oxid. Med. Cell. Longev., 3279670. es_ES
dc.description.references https://doi.org/10.1155/2019/3279670 es_ES
dc.description.references Chan P.J., Corselli J.U., Jacobson J.D., Patton W.C., King A. 1991. Spermac stain analysis of human sperm acrosomes. Fertil. Steril., 72: 124-128. https://doi.org/10.1016/S0015-0282(99)00201-0 es_ES
dc.description.references Chanapiwat P., Kaeoket K., Tummaruk P. 2009. Effects of DHAenriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen. Asian J. Androl., 11: 600-608. https://doi.org/10.1038/aja.2009.40 es_ES
dc.description.references Cheema R.S., Bansal A.K., Bilaspuri G.S. 2009. Manganese provides antioxidant protection for sperm cryopreservation that may offer new consideration for clinical fertility. Oxid. Med. Cell. Longev., 2: 152-159. https://doi.org/10.4161/oxim.2.3.8804 es_ES
dc.description.references Conquer J.A., Martin J.B., Tummon I., Watson L., Tekpetey F. 1999. Fatty acid analysis of blood serum, seminal plasma, and spermatozoa of normozoospermic vs. asthenozoospermic males. Lipids, 34: 793-799. https://doi.org/10.1007/s11745-999-0425-1 es_ES
dc.description.references Conquer J.A., Martin J.B., Tummon I., Watson L., Tekpetey F. 2000. Effect of DHA supplementation on DHA status and sperm motility in asthenozoospermic males. Lipids, 35: 149-154. https://doi.org/10.1007/BF02664764 es_ES
dc.description.references Cristanelli M.J., Amann R.P., Squires E.L., Pickett B.W. 1985. Effects of egg yolk and glycerol levels in lactose-EDTA-egg yolk extender and of freezing rate on the motility of frozenthawed stallion spermatozoa. Theriogenology, 24: 681-686. es_ES
dc.description.references https://doi.org/10.1016/0093-691X(85)90166-9 es_ES
dc.description.references Di Iorio M., Colonna M.A., Miranda M., Principe P., Schiavitto M., Cerolini S., Manchisi A., Iaffaldano N. 2018. Initial cooling time before freezing affects post-thaw quality and reproductive performance of rabbit semen. Anim. Sci. J., 89: 1240-1244. es_ES
dc.description.references https://doi.org/10.1111/asj.13046 es_ES
dc.description.references Dolatpanah M.B., Towhidi A., Farshad A., Rashidi A., Rezayazdi K. 2008. Effects of dietary fish oil on semen quality of goats. Asian-australas. J. Anim. Sci.,21: 29-34. https://doi.org/10.5713/ajas.2008.70035 es_ES
dc.description.references Erickson M.C. 1998. Chemistry and function of phospholipids. Food lipids, chemistry, nutrition and biochemistry manual, p. 41. es_ES
dc.description.references Evans G., Maxwell W.M.C. 1987. Salamon’s artificial insemination of sheep and goats. Sydney: Butterworths. 194 pp. es_ES
dc.description.references Fadl A., Ghallab A., Abou-Ahmed M. 2019. Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants. World Rabbit Sci., 27: 77-83. https://doi.org/10.4995/wrs.2019.10892 es_ES
dc.description.references Gholami H., Chamani M., Towhidi A., Fazeli M.H. 2010. Effect of feeding a docosahexaenoic acid-enriched nutriceutical on the quality of fresh and frozen-thawed semen in Holstein bulls. Theriogenology, 74: 1548-1558. https://doi.org/10.1016/j.theriogenology.2010.06.025 es_ES
dc.description.references Goldberg D.M., Spooner R.J. 1974. Methods of enzymatic analysis. In: BERGMEYER HU (Ed.), 3rd ed. New York: Academic Press, 258-265. es_ES
dc.description.references Gürler H., Calisici O., Calisici D., Bollwein H. 2015. Effects of feeding omega-3-fatty acids on fatty acid composition and quality of bovine sperm and on antioxidative capacity of bovine seminal plasma. Anim. Reprod. Sci., 160: 97-104. https://doi.org/10.1016/j.anireprosci.2015.07.010 es_ES
dc.description.references Iaffaldano N., Di Iorio M., Rosato M.P., Manchisi A. 2014. Cryopreservation of rabbit semen using non-permeable cryoprotectants: effectiveness of different concentrations of low-density lipoproteins (LDL) from egg yolk versus egg yolk or sucrose. Anim Reprod Sci., 151: 220-228. https://doi.org/10.1016/j.anireprosci.2014.10.020 es_ES
dc.description.references James P., Wolfe C., Mackie A., Ladha S., Prentica A., Jones R. 1999. Lipid dynamics in the plasma membrane of fresh and preserved human spermatozoa. Hum. Reprod.,14: 1827-1832. https://doi.org/10.1093/humrep/14.7.1827 es_ES
dc.description.references Kaka A., Wahid H., Rosnina Y., Yimer N., Khumran A.M., Behan A.A., Ebrahimi M. 2015a. Alpha-linolenic acid supplementation in tris extender can improve frozen-thawed bull semen quality. Reprod. Domest. Anim., 50: 29-33. https://doi.org/10.1111/rda.12445 es_ES
dc.description.references Kaka A., Wahid H., Rosnina Y., Yimer N., Khumran A.M., Sarsaifi K., Behan A.A., Kaka U., Ebrahimi M. 2015b. α-Linolenic acid supplementation in BioXcell® extender can improve the quality of post-cooling and frozenthawed bovine sperm. Anim. Reprod. Sci., 153: 1-7. https://doi.org/10.1016/j.anireprosci.2014.12.001 es_ES
dc.description.references Kandelousi M.A., Arshami J., Naserian A.A., Abavisani A. 2013. The effects of addition of omega-3, 6, 9 fatty acids on the quality of bovine chilled and frozen-thawed sperm. Open Vet. J., 3: 47-52. es_ES
dc.description.references Kiernan M., Fahey A.G., Fair S. 2013. The effect of the in vitro supplementation of exogenous long-chain fatty acids on bovine sperm cell function. Reprod. Fertil. Dev., 25: 947-954. https://doi.org/10.1071/RD12204 es_ES
dc.description.references Lenzi A., Gandini L., Lombardo F., Picardo M., Maresca V., Panfili E., Tramer F., Boitani C., Dondero F. 2002. Polyunsaturated fatty acids of germ cell membranes, glutathione and blutathione-dependent enzyme-PHGPx: from basic to clinic. Contraception, 65: 301-304. es_ES
dc.description.references https://doi.org/10.1016/S0010-7824(02)00276-7 es_ES
dc.description.references Lessard C., Parent S., Leclerc P., Bailey J.L., Sullivan R. 2000. Cryopreservation alters the levels of the bull sperm surface protein P25b. J. Androl., 21: 700-707. es_ES
dc.description.references Maldjian A., Pizzi F., Gliozzi T., Cerolini S., Penny P., Noble R. 2005. Changes in sperm quality and lipid composition during cryopreservation of boar semen. Theriogenology, 63: 411-421. https://doi.org/10.1016/j.theriogenology.2004.09.021 es_ES
dc.description.references Masoudi R., Sharafi M., Zare Shahneh A., Towhidi A., Kohram H., Zhandi M., Esmaeili V., Shahverdi A. 2016. Effect of dietary fish oil supplementation on ram semen freeze ability and fertility using soybean lecithin- and egg yolkbased extenders. Theriogenology, 86: 1583-1588. https://doi.org/10.1016/j.theriogenology.2016.05.018 es_ES
dc.description.references Medeiros C.M., Forell F., Oliveira A.T., Rodrigues J.L. 2002. Current status of sperm cryopreservation: why isn’t it better? Theriogenology, 57: 327-344. https://doi.org/10.1016/S0093-691X(01)00674-4 es_ES
dc.description.references Mocé E., Vicente J.S. 2009. Rabbit sperm cryopreservation: a review. Anim. Reprod. Sci., 110: 1-24. https://doi.org/10.1016/j.anireprosci.2008.08.015 es_ES
dc.description.references NATIONAL RESEARCH COUNCIL. 1977. Nutrient Requirements of Rabbits,: Second Revised Edition, Washington, DC: The National Academies Press. es_ES
dc.description.references Nasiri A.H., Towhidi A., Zeinoaldini S. 2012. Combined effect of DHA and α-tocopherol supplementation during bull semen cryopreservation on sperm characteristics and fatty acid composition. Andrologia, 1: 550-555. es_ES
dc.description.references https://doi.org/10.1111/j.1439-0272.2011.01225.x es_ES
dc.description.references Nishikimi M., Appaji N., Yagi K. 1972. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem. Biophys. Res. Commun., 46: 849-854. es_ES
dc.description.references Ohkawa H., Ohishi N., Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 95: 351-358. https://doi.org/10.1016/0003-2697(79)90738-3 es_ES
dc.description.references Robinson J.J., Ashworth C.J., Rooke J.A., Mitchell L.M., McEvoy T.G. 2006. Nutrition and fertility in ruminant livestock. Anim. Feed Sci. Technol.,126: 259-276. https://doi.org/10.1016/j.anifeedsci.2005.08.006 es_ES
dc.description.references Roca J., Martínez S., Vázquez J.M., Lucas X., Parrilla I., Martínez E.A. 2000. Viability and fertility of rabbit spermatozoa diluted in Tris-buffer extenders and stored at 15 degrees C. Anim.Reprod. Sci., 64: 103-112. https://doi.org/10.1016/S0378-4320(00)00185-8 es_ES
dc.description.references Rodrigues P.G., de Moura R.S., Rocha L.G., Bottino M., Nichi M., Maculan R., Bertechini A.G., Souza J.C. 2017. Dietary Polyunsaturated Fatty Acid Supplementation Improves the Quality of Stallion Cryopreserved Semen. J. Equine Vet. Sci., 54: 18-23. https://doi.org/10.1016/j.jevs.2016.08.007 es_ES
dc.description.references Rooke J.A., Shao C.C., Speake B.K. 2001. Effects of feeding tuna oil on the lipid composition of pig spermatozoa and in vitro characteristics of semen. Reproduction, 121: 315-322. https://doi.org/10.1530/rep.0.1210315 es_ES
dc.description.references Samadian F., Towhidi A., Rezayazdi K., Bahreini M. 2010. Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm. Animal, 4: 2017-2022. https://doi.org/10.1017/S1751731110001308 es_ES
dc.description.references Shevchenko A., Simons K. 2010. Lipidomics: coming to grips with lipid diversity. Nat.Rev. Mol. Cell. Biol., 11: 593-598. https://doi.org/10.1038/nrm2934 es_ES
dc.description.references Towhidi A., Zeinoaldini S., Ardebili R., Dadashpour Davachi N., Nasiri A. 2013. Combined n-3 Fatty Acids and α-Tocopherol Supplementation Improved the Ovine Sperm Cryosurvival. Iran. J. Biotechnol., 11: 238-243. https://doi.org/10.5812/ijb.14469 es_ES
dc.description.references Watson P.F. 2000. The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci., 60-61: 481-492. https://doi.org/10.1016/S0378-4320(00)00099-3 es_ES
dc.description.references Yimer N., Noraisyah A.H., Rosnina Y., Wahid H., Sarsaifi K., Hafiza A.M. 2014. Comparison of cryopreservative effect of different levels of omega-3 egg-yolk in citrate extender on the quality of goat spermatozoa. Pak. Vet. J., 34: 347-350. es_ES
dc.description.references Yoshida M. 2000. Conservation of sperms: current status and new trends. Anim. Reprod. Sci., 60-61: 349-355. https://doi.org/10.1016/S0378-4320(00)00125-1 es_ES


Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem