Resumen:
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[EN] Plant survival depends on the optimal use of resources under variable environmental conditions. Among the mechanisms that mediate the balance between growth, differentiation, and stress responses, the regulation of ...[+]
[EN] Plant survival depends on the optimal use of resources under variable environmental conditions. Among the mechanisms that mediate the balance between growth, differentiation, and stress responses, the regulation of transcriptional activity by DELLA proteins stands out. In angiosperms, DELLA accumulation promotes defense against biotic and abiotic stress and represses cell division and expansion, while the loss of DELLA function is associated with increased plant size and sensitivity toward stress.(1) Given that DELLA protein stability is dependent on gibberellin (GA) levels(2) and GA metabolism is influenced by the environment,(3) this pathway is proposed to relay environmental information to the transcriptional programs that regulate growth and stress responses in angiosperms. (4,5)However, DELLA genes are also found in bryophytes, whereas canonical GA receptors have been identified only in vascular plants(.6-10) Thus, it is not clear whether these regulatory functions of DELLA predated or emerged with typical GA signaling. Here, we show that, as in vascular plants, the only DELLA in the liverwort Marchantia polymorpha also participates in the regulation of growth and key developmental processes and promotes oxidative stress tolerance. Moreover, part of these effects is likely caused by the conserved physical interaction with the MpPIF transcription factor Therefore, we suggest that the role in the coordination of growth and stress responses was already encoded in the DELLA protein of the common ancestor of land plants, and the importance of this function is underscored by its conservation over the past 450 million years.
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Agradecimientos:
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We thank Eri Nakamura for the pENTR1A-proMpCYCB;1-Dbox plasmid; Shuji Shigenobu and Katsushi Yamaguchi in National Institute for Basic Biology for the help in RNA-seq of Mppifko; Roberto Solano, Maite Sanmartn, and all ...[+]
We thank Eri Nakamura for the pENTR1A-proMpCYCB;1-Dbox plasmid; Shuji Shigenobu and Katsushi Yamaguchi in National Institute for Basic Biology for the help in RNA-seq of Mppifko; Roberto Solano, Maite Sanmartn, and all members of the Plant Plasticity Lab for discussions and insightful commentson the manuscript; and Lynne Yenush for language editing. Work in the au-thors' laboratories has been funded by the Spanish Agencia Estatal de Inves-tigacion and FEDER (grants BFU2016-80621-P and PID2019-110717GB to M.A.B.) and JSPS/MEXT KAKENHI (JP17H07424 and JP19H05675 to T.K.; 20H04884 to R.N.) . J.H.-G. and A.S.-M. were supported by a predoctoral fellowship from the Spanish Ministry of Education, Culture and Sport (FPU15/01756) and an MSCA Individual Fellowship (H2020-MSCA-IF-2016-746396) , respectively. R.S. was supported by the Japanese Government (MEXT) Scholarship Program.
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