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Expression of an extremophilic xylanase in Nicotiana benthamiana and its use for the production of prebiotic xylooligosaccharides

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Expression of an extremophilic xylanase in Nicotiana benthamiana and its use for the production of prebiotic xylooligosaccharides

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dc.contributor.author Talens-Perales, David es_ES
dc.contributor.author Nicolau-Sanus, Maria es_ES
dc.contributor.author Polaina, Julio es_ES
dc.contributor.author Daròs, José-Antonio es_ES
dc.date.accessioned 2023-05-12T18:01:54Z
dc.date.available 2023-05-12T18:01:54Z
dc.date.issued 2022-09-21 es_ES
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://hdl.handle.net/10251/193317
dc.description.abstract [EN] A gene construct encoding a xylanase, which is active in extreme conditions of temperature and alkaline pH (90 °C, pH 10.5), has been transitorily expressed with high efficiency in Nicotiana benthamiana using a viral vector. The enzyme, targeted to the apoplast, accumulates in large amounts in plant tissues in as little as 7 days after inoculation, without detrimental effects on plant growth. The properties of the protein produced by the plant, in terms of resistance to temperature, pH, and enzymatic activity, are equivalent to those observed when Escherichia coli is used as a host. Purification of the plant-produced recombinant xylanase is facilitated by exporting the protein to the apoplastic space. The production of this xylanase by N. benthamiana, which avoids the hindrances derived from the use of E. coli, namely, intracellular production requiring subsequent purification, represents an important step for potential applications in the food industry in which more sustainable and green products are continuously demanded. As an example, the use of the enzyme producing prebiotic xylooligosdaccharides from xylan is here reported. es_ES
dc.description.sponsorship This work was supported by grant PID2020-114691RB-I00 from the Spanish Ministerio de Ciencia e Innovacion, through the Agencia Estatal de Investigacion (co-financed European Regional Development Fund), and by the Bio Based Industries Joint Undertaking, under the European Union's Horizon 2020 research and innovation program (Project WOODZYMES, Grant Agreement H2020-BBI-JU-792070). M.N.-S. is the recipient of a predoctoral contract from the Spanish Ministerio de Ciencia e Innovacion (PRE2018-084771). es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Biochemistry es_ES
dc.subject Biotechnology es_ES
dc.subject Plant sciences es_ES
dc.subject Microbiology es_ES
dc.title Expression of an extremophilic xylanase in Nicotiana benthamiana and its use for the production of prebiotic xylooligosaccharides es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s41598-022-19774-5 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114691RB-I00/ES/BIOTECNOLOGIA DE VIRUS DE PLANTAS: VECTORES VIRALES Y ESTRATEGIAS DE RESISTENCIA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//PRE2018-084771/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/792070/EU es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Talens-Perales, D.; Nicolau-Sanus, M.; Polaina, J.; Daròs, J. (2022). Expression of an extremophilic xylanase in Nicotiana benthamiana and its use for the production of prebiotic xylooligosaccharides. Scientific Reports. 12(1):1-10. https://doi.org/10.1038/s41598-022-19774-5 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s41598-022-19774-5 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 10 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 12 es_ES
dc.description.issue 1 es_ES
dc.identifier.pmid 36131073 es_ES
dc.identifier.pmcid PMC9492658 es_ES
dc.relation.pasarela S\473471 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
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