Climent Terol, E.; Biyikal, M.; Gröninger, D.; Weller, MG.; Martínez-Máñez, R.; Rurack, K. (2020). Multiplexed Detection of Analytes on Single Test Strips with Antibody-Gated Indicator-Releasing Mesoporous Nanoparticles. Angewandte Chemie International Edition. 59(52):23862-23869. https://doi.org/10.1002/anie.202009000
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/193467
Título:
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Multiplexed Detection of Analytes on Single Test Strips with Antibody-Gated Indicator-Releasing Mesoporous Nanoparticles
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Autor:
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Climent Terol, Estela
Biyikal, Mustafa
Gröninger, Delia
Weller, Michael G.
Martínez-Máñez, Ramón
Rurack, Knut
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Entidad UPV:
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Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials
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Fecha difusión:
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Resumen:
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[EN] Rapid testing methods for the use directly at a point of need are expected to unfold their true potential especially when offering adequate capabilities for the simultaneous measurement of multiple analytes of interest. ...[+]
[EN] Rapid testing methods for the use directly at a point of need are expected to unfold their true potential especially when offering adequate capabilities for the simultaneous measurement of multiple analytes of interest. Considering the unique modularity, high sensitivity, and selectivity of antibody-gated indicator delivery (gAID) systems, a multiplexed assay for three small-molecule explosives (TATP, TNT, PETN) was thus developed, allowing to detect the analytes simultaneously with a single test strip at lower ppb concentrations in the liquid phase in <5 min using a fluorescence reader or a smartphone for readout. While the TNT and PETN systems were newly developed here, all the three systems also tolerated harsher matrices than buffered aqueous model solutions. Besides a single-track strip, the outstanding modularity of the hybrid biosensor materials in combination with strip-patterning technologies allowed us to obtain a multichannel strip in a straightforward manner, offering comparable analytical performance while allowing to be tailored even more to the user's need.
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Palabras clave:
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Explosives
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Hybrid materials
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Multiplexing
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Signal amplification
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Test-strip analysis
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Derechos de uso:
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Reconocimiento (by)
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Fuente:
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Angewandte Chemie International Edition. (issn:
1433-7851
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DOI:
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10.1002/anie.202009000
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Editorial:
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John Wiley & Sons
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Versión del editor:
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https://doi.org/10.1002/anie.202009000
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Código del Proyecto:
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info:eu-repo/grantAgreement/AEI//RTI2018-100910-B-C41-AR//MATERIALES POROSOS INTELIGENTES MULTIFUNCIONALES Y DSPOSITIVOS ELECTRONICOS PARA LA LIBERACION DE FARMACOS, DETECCION DE DROGAS Y BIOMARCADORES Y COMUNICACION A NANOESCALA/
info:eu-repo/grantAgreement/GENERALITAT VALENCIANA//PROMETEO%2F2018%2F024//SISTEMAS AVANZADOS DE LIBERACION CONTROLADA/
info:eu-repo/grantAgreement/BMWI//DEmONS 06%2F12/
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Descripción:
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This is the peer reviewed version of the following article: Climent, E., Biyikal, M., Gröninger, D., Weller, M. G., Martínez¿Máñez, R., & Rurack, K. (2020). Multiplexed Detection of Analytes on Single Test Strips with Antibody-Gated Indicator-Releasing Mesoporous Nanoparticles. Angewandte Chemie International Edition, 59(52), 23862-23869, which has been published in final form at https://doi.org/10.1002/anie.202009000. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."
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Agradecimientos:
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Financial support from the Alexander von Humboldt Foundation (fellowship to E.C.), Germany's Federal Ministry for Economic Affairs and Energy (project DEmONS 06/12), the Spanish Government (project RTI2018-100910-B-C41 ...[+]
Financial support from the Alexander von Humboldt Foundation (fellowship to E.C.), Germany's Federal Ministry for Economic Affairs and Energy (project DEmONS 06/12), the Spanish Government (project RTI2018-100910-B-C41 (MCUI/FEDER, EU)) and the Generalitat Valenciana (project PROMETEO 2018/024) is gratefully acknowledged. We thank S. Selve (Technical University Berlin) for TEM images, A. Zehl (Humboldt University Berlin) for elemental analysis, D. Pfeifer and C. Jager (BAM, Structural Analysis Division) for NMR support, A. Zimathies (BAM, Structural Analysis Division) for N<INF>2</INF> adsorption/desorption measurements, Y. Salinas and L. E. Santos for support on the materials screening, A. Walter, S. Ramin and A. Hesse for obtaining the sera and R. Gotor and J. Bell for their help in the fabrication of the home-made smartphone periphery. Open access funding enabled and organized by Projekt DEAL.
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Tipo:
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Artículo
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