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dc.contributor.author | Clara-Trujillo, Sandra![]() |
es_ES |
dc.contributor.author | Tolosa, Laia![]() |
es_ES |
dc.contributor.author | Cordón, Lourdes![]() |
es_ES |
dc.contributor.author | Sempere, Amparo![]() |
es_ES |
dc.contributor.author | Gallego-Ferrer, Gloria![]() |
es_ES |
dc.contributor.author | Gómez Ribelles, José Luís![]() |
es_ES |
dc.date.accessioned | 2023-10-05T18:01:54Z | |
dc.date.available | 2023-10-05T18:01:54Z | |
dc.date.issued | 2022-04 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/197775 | |
dc.description.abstract | [EN] Multiple myeloma (MM) is a hematological malignancy in which the patient's drug resistance is one of the main clinical problems. As 2D cultures do not recapitulate the cellular microenvironment, which has a key role in drug resistance, there is an urgent need for better biomimetic models. Here, a novel 3D platform is used to model MM. The semi-solid culture consists of a dynamic suspension of microspheres and MM cells, termed as microgel. Microspheres are synthesized with acrylic polymers of different sizes, compositions, and functionalities (fibronectin or hyaluronic acid). Optimal conditions for the platform in terms of agitation speed and microsphere size have been determined. With these parameters the system allows good proliferation of the MM cell lines RPMI8226, U226, and MM1.S. Interestingly, when used for drug resistance studies, culture of the three MM cell lines in microgels showed close agreement in revealing the role of acrylic acid in resistance to anti-MM drugs such as dexamethasone and bortezomib. This work presents a unique platform for the in vitro modeling of non-solid tumors since it allows keeping non-adherent cells in suspension conditions but in a 3D context that can be easily tuned with different functionalizations. | es_ES |
dc.description.sponsorship | This work was funded by the Spanish State Research Agency (AEI) through the PID2019-106099RB-C41/AEI/10.13039/501100011033 Project. CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER Actions were financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. This work was also supported by the Spanish Ministry of Science, Innovation and Universities through Grant NO FPU17/05810 awarded to Sandra Clara-Trujillo. The Microscopy Service of the UPV (UniversitatPolitecnica de València) is gratefully acknowledged for helping with FESEM characterization. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Biomaterials Advances | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Multiple myeloma | es_ES |
dc.subject | Microgels | es_ES |
dc.subject | Microspheres | es_ES |
dc.subject | Emulsion polymerization | es_ES |
dc.subject | Drug resistance | es_ES |
dc.subject | Acrylic acid | es_ES |
dc.subject.classification | MAQUINAS Y MOTORES TERMICOS | es_ES |
dc.title | Novel microgel culture system as semi-solid three-dimensional in vitro model for the study of multiple myeloma proliferation and drug resistance | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.bioadv.2022.212749 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106099RB-C41/ES/MICROGELES BIOMIMETICOS PARA EL ESTUDIO DE LA GENERACION DE RESISTENCIAS A FARMACOS EN EL MIELOMA MULTIPLE/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ //FPU17%2F05810//AYUDA CONTRATO PREDOCTORAL FPU-CLARA TRUJILLO. PROYECTO: PERSONALIZED MEDICINE DEVICES FOR BLOOD CANCERS/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials | es_ES |
dc.description.bibliographicCitation | Clara-Trujillo, S.; Tolosa, L.; Cordón, L.; Sempere, A.; Gallego-Ferrer, G.; Gómez Ribelles, JL. (2022). Novel microgel culture system as semi-solid three-dimensional in vitro model for the study of multiple myeloma proliferation and drug resistance. Biomaterials Advances. 135:1-13. https://doi.org/10.1016/j.bioadv.2022.212749 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.bioadv.2022.212749 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 13 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 135 | es_ES |
dc.identifier.eissn | 2772-9508 | es_ES |
dc.identifier.pmid | 35929221 | es_ES |
dc.relation.pasarela | S\461744 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | AGENCIA ESTATAL DE INVESTIGACION | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Universitat Politècnica de València | es_ES |
dc.contributor.funder | MINISTERIO DE CIENCIA INNOVACION Y UNIVERSIDADES | es_ES |