- -

Photonic Label-Free Biosensors for Fast and Multiplex Detection of Swine Viral Diseases

RiuNet: Repositorio Institucional de la Universidad Politécnica de Valencia

Compartir/Enviar a

Citas

Estadísticas

  • Estadisticas de Uso

Photonic Label-Free Biosensors for Fast and Multiplex Detection of Swine Viral Diseases

Mostrar el registro sencillo del ítem

Ficheros en el ítem

dc.contributor.author Gómez-Gómez, María Isabel es_ES
dc.contributor.author Sánchez, Carles es_ES
dc.contributor.author Peransi, Sergio es_ES
dc.contributor.author Zurita, David es_ES
dc.contributor.author Bellieres, Laurent es_ES
dc.contributor.author Recuero, Sara es_ES
dc.contributor.author Rodrigo, Manuel es_ES
dc.contributor.author Simón, Santiago es_ES
dc.contributor.author Camarca, Alessandra es_ES
dc.contributor.author Capo, Alessandro es_ES
dc.contributor.author Staiano, Maria es_ES
dc.contributor.author Varriale, Antonio es_ES
dc.contributor.author D Auria, Sabato es_ES
dc.contributor.author Manessis, Georgios es_ES
dc.contributor.author Gelasakis, Athnasios I. es_ES
dc.contributor.author Griol Barres, Amadeu es_ES
dc.date.accessioned 2023-10-23T18:00:59Z
dc.date.available 2023-10-23T18:00:59Z
dc.date.issued 2022-02 es_ES
dc.identifier.uri http://hdl.handle.net/10251/198602
dc.description.abstract [EN] In this paper we present the development of photonic integrated circuit (PIC) biosensors for the label-free detection of six emerging and endemic swine viruses, namely: African Swine Fever Virus (ASFV), Classical Swine Fever Virus (CSFV), Porcine Reproductive and Respiratory Syndrome Virus (PPRSV), Porcine Parvovirus (PPV), Porcine Circovirus 2 (PCV2), and Swine Influenza Virus A (SIV). The optical biosensors are based on evanescent wave technology and, in particular, on Resonant Rings (RRs) fabricated in silicon nitride. The novel biosensors were packaged in an integrated sensing cartridge that included a microfluidic channel for buffer/sample delivery and an optical fiber array for the optical operation of the PICs. Antibodies were used as molecular recognition elements (MREs) and were selected based on western blotting and ELISA experiments to ensure the high sensitivity and specificity of the novel sensors. MREs were immobilized on RR surfaces to capture viral antigens. Antibody-antigen interactions were transduced via the RRs to a measurable resonant shift. Cell culture supernatants for all of the targeted viruses were used to validate the biosensors. Resonant shift responses were dose-dependent. The results were obtained within the framework of the SWINOSTICS project, contributing to cover the need of the novel diagnostic tools to tackle swine viral diseases. es_ES
dc.description.sponsorship This work was funded by the EU-2020 program under grant agreement Nº 771649-SWINOSTICS project. es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Sensors es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Swine disease es_ES
dc.subject Photonics es_ES
dc.subject Antibody es_ES
dc.subject Ring resonator es_ES
dc.subject Photonic integrated circuit (PIC) es_ES
dc.subject Biosensor es_ES
dc.subject Label-free es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Photonic Label-Free Biosensors for Fast and Multiplex Detection of Swine Viral Diseases es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/s22030708 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/771649/EU es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros de Telecomunicación - Escola Tècnica Superior d'Enginyers de Telecomunicació es_ES
dc.contributor.affiliation Universitat Politècnica de València. Área de la Ciudad Politécnica de la Innovación - Àrea de la Ciutat Politècnica de la Innovació es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Tecnología Nanofotónica - Institut Universitari de Tecnologia Nanofotònica es_ES
dc.description.bibliographicCitation Gómez-Gómez, MI.; Sánchez, C.; Peransi, S.; Zurita, D.; Bellieres, L.; Recuero, S.; Rodrigo, M.... (2022). Photonic Label-Free Biosensors for Fast and Multiplex Detection of Swine Viral Diseases. Sensors. 22(3):1-14. https://doi.org/10.3390/s22030708 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/s22030708 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 14 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 22 es_ES
dc.description.issue 3 es_ES
dc.identifier.eissn 1424-8220 es_ES
dc.identifier.pmid 35161454 es_ES
dc.identifier.pmcid PMC8838678 es_ES
dc.relation.pasarela S\455763 es_ES
dc.contributor.funder COMISION DE LAS COMUNIDADES EUROPEA es_ES
upv.costeAPC 2178 es_ES


Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem