Arrones-Olmo, A.; Mangino, G.; Alonso-Martín, D.; Plazas Ávila, MDLO.; Prohens Tomás, J.; Portis, E.; Barchi, L.... (2022). Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette. Frontiers in Plant Science. 13:1-14. https://doi.org/10.3389/fpls.2022.1025951
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/198749
Title:
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Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette
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Author:
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Arrones-Olmo, Andrea
Mangino, Giulio
Alonso-Martín, David
Plazas Ávila, María de la O
Prohens Tomás, Jaime
Portis, Ezio
Barchi, Lorenzo
Giuliano, Giovanni
Vilanova Navarro, Santiago
Gramazio, Pietro
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UPV Unit:
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Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana
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Issued date:
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Abstract:
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[EN] Understanding the mechanisms by which chlorophylls are synthesized in the eggplant (Solanum melongena) fruit peel is of great relevance for eggplant breeding. A multi-parent advanced generation inter-cross (MAGIC) ...[+]
[EN] Understanding the mechanisms by which chlorophylls are synthesized in the eggplant (Solanum melongena) fruit peel is of great relevance for eggplant breeding. A multi-parent advanced generation inter-cross (MAGIC) population and a germplasm collection have been screened for green pigmentation in the fruit peel and used to identify candidate genes for this trait. A genome-wide association study (GWAS) performed with 420 MAGIC individuals revealed a major association on chromosome 8 close to a gene similar to APRR2. Two variants in SmAPRR2, predicted as having a high impact effect, were associated with the absence of fruit chlorophyll pigmentation in the MAGIC population, and a large deletion of 5.27 kb was found in two reference genomes of accessions without chlorophyll in the fruit peel. The validation of the candidate gene SmAPRR2 was performed by its sequencing in a set of MAGIC individuals and through its de novo assembly in 277 accessions from the G2P-SOL eggplant core collection. Two additional mutations in SmAPRR2 associated with the lack of chlorophyll were identified in the core collection set. The phylogenetic analysis of APRR2 reveals orthology within Solanaceae and suggests that specialization of APRR2-like genes occurred independently in Cucurbitaceae and Solanaceae. A strong geographical differentiation was observed in the frequency of predominant mutations in SmAPRR2, resulting in a lack of fruit chlorophyll pigmentation and suggesting that this phenotype may have arisen and been selected independently several times. This study represents the first identification of a major gene for fruit chlorophyll pigmentation in the eggplant fruit.
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Subjects:
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Fruit peel chlorophyll pigmentation
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Eggplant (Solanum melongena)
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SmAPRR2
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Multi-parent advanced generation inter-cross (MAGIC) population
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Fruit colour diversification
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Genome-wide association study (GWAS)
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Copyrigths:
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Reconocimiento (by)
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Source:
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Frontiers in Plant Science. (eissn:
1664-462X
)
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DOI:
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10.3389/fpls.2022.1025951
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Publisher:
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Frontiers Media SA
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Publisher version:
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https://doi.org/10.3389/fpls.2022.1025951
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Coste APC:
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3412,87 €
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Project ID:
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094592-B-I00/ES/INTROGRESION DE TOLERANCIA A LA SEQUIA PROCEDENTE DE ESPECIES SILVESTRES PARA LA MEJORA GENETICA DE LA BERENJENA/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-128148OB-I00/ES/UTILIZACION DE LINEAS DE INTROGRESION Y MAGIC EN LA MEJORA GENETICA E IDENTIFICACION DE GENES DE CARACTERES CLAVE EN BERENJENA/
info:eu-repo/grantAgreement/EC/H2020/677379/EU
info:eu-repo/grantAgreement/MICIU//FPU18%2F01742/ES/DESARROLLO Y UTILIZACION DE POBLACIONES EXPERIMENTALES MAGIC EN LA MEJORA DE SOLANACEAS/
info:eu-repo/grantAgreement/MICINN/Juan de la Cierva-Formación/FJC2019-038921-I/ES/Edición genética aplicada a la mejora de cultivos industriales
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Thanks:
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This work has been funded by grants RTI-2018-094592-BI00 and PID2021-128148OB-I00 funded by MCIN/AEI/10.13039/501100011033/and by "ERDF A way of making Europe", and by European Union's Horizon 2020 Research and Innovation ...[+]
This work has been funded by grants RTI-2018-094592-BI00 and PID2021-128148OB-I00 funded by MCIN/AEI/10.13039/501100011033/and by "ERDF A way of making Europe", and by European Union's Horizon 2020 Research and Innovation Programme under Grant Agreement No. 677379 (G2P-SOL project: Linking genetic resources, genomes and phenotypes of Solanaceous crops). AA is grateful to Spanish Ministerio de Ciencia, Innovación y Universidades for a predoctoral (FPU18/01742) contract. PG is grateful to Spanish Ministerio de Ciencia e Innovacion for a post-doctoral grant (FJC2019-038921-I/AEI/10.13039/501100011033). Funding for open access charge: Universitat Politecnica de Valencia.
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Type:
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Artículo
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