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Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette

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Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette

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dc.contributor.author Arrones-Olmo, Andrea es_ES
dc.contributor.author Mangino, Giulio es_ES
dc.contributor.author Alonso-Martín, David es_ES
dc.contributor.author Plazas Ávila, María de la O es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.contributor.author Portis, Ezio es_ES
dc.contributor.author Barchi, Lorenzo es_ES
dc.contributor.author Giuliano, Giovanni es_ES
dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.contributor.author Gramazio, Pietro es_ES
dc.date.accessioned 2023-10-24T18:01:35Z
dc.date.available 2023-10-24T18:01:35Z
dc.date.issued 2022-10-17 es_ES
dc.identifier.uri http://hdl.handle.net/10251/198749
dc.description.abstract [EN] Understanding the mechanisms by which chlorophylls are synthesized in the eggplant (Solanum melongena) fruit peel is of great relevance for eggplant breeding. A multi-parent advanced generation inter-cross (MAGIC) population and a germplasm collection have been screened for green pigmentation in the fruit peel and used to identify candidate genes for this trait. A genome-wide association study (GWAS) performed with 420 MAGIC individuals revealed a major association on chromosome 8 close to a gene similar to APRR2. Two variants in SmAPRR2, predicted as having a high impact effect, were associated with the absence of fruit chlorophyll pigmentation in the MAGIC population, and a large deletion of 5.27 kb was found in two reference genomes of accessions without chlorophyll in the fruit peel. The validation of the candidate gene SmAPRR2 was performed by its sequencing in a set of MAGIC individuals and through its de novo assembly in 277 accessions from the G2P-SOL eggplant core collection. Two additional mutations in SmAPRR2 associated with the lack of chlorophyll were identified in the core collection set. The phylogenetic analysis of APRR2 reveals orthology within Solanaceae and suggests that specialization of APRR2-like genes occurred independently in Cucurbitaceae and Solanaceae. A strong geographical differentiation was observed in the frequency of predominant mutations in SmAPRR2, resulting in a lack of fruit chlorophyll pigmentation and suggesting that this phenotype may have arisen and been selected independently several times. This study represents the first identification of a major gene for fruit chlorophyll pigmentation in the eggplant fruit. es_ES
dc.description.sponsorship This work has been funded by grants RTI-2018-094592-BI00 and PID2021-128148OB-I00 funded by MCIN/AEI/10.13039/501100011033/and by "ERDF A way of making Europe", and by European Union's Horizon 2020 Research and Innovation Programme under Grant Agreement No. 677379 (G2P-SOL project: Linking genetic resources, genomes and phenotypes of Solanaceous crops). AA is grateful to Spanish Ministerio de Ciencia, Innovacio ' n y Universidades for a predoctoral (FPU18/01742) contract. PG is grateful to Spanish Ministerio de Ciencia e Innovacion for a post-doctoral grant (FJC2019-038921-I/AEI/10.13039/501100011033). Funding for open access charge: Universitat Politecnica de Valencia. es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Plant Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Fruit peel chlorophyll pigmentation es_ES
dc.subject Eggplant (Solanum melongena) es_ES
dc.subject SmAPRR2 es_ES
dc.subject Multi-parent advanced generation inter-cross (MAGIC) population es_ES
dc.subject Fruit colour diversification es_ES
dc.subject Genome-wide association study (GWAS) es_ES
dc.subject.classification GENETICA es_ES
dc.title Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fpls.2022.1025951 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094592-B-I00/ES/INTROGRESION DE TOLERANCIA A LA SEQUIA PROCEDENTE DE ESPECIES SILVESTRES PARA LA MEJORA GENETICA DE LA BERENJENA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//PID2021-128148OB-I00//UTILIZACIÓN DE LÍNEAS DE INTROGRESIÓN Y MAGIC EN LA MEJORA GENÉTICA E IDENTIFICACIÓN DE GENES DE CARACTERES CLAVE EN BERENJENA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/677379/EU es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ //FPU18%2F01742//AYUDA PREDOCTORAL FPU-ARRONES OLMO. PROYECTO: DESARROLLO Y UTILIZACION DE POBLACIONES EXPERIMENTALES MAGIC EN LA MEJORA DE SOLANACEAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//FJC2019-038921-I//Juan de la Cierva-Formación post-doctoral grant/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Arrones-Olmo, A.; Mangino, G.; Alonso-Martín, D.; Plazas Ávila, MDLO.; Prohens Tomás, J.; Portis, E.; Barchi, L.... (2022). Mutations in the SmAPRR2 transcription factor suppressing chlorophyll pigmentation in the eggplant fruit peel are key drivers of a diversified colour palette. Frontiers in Plant Science. 13:1-14. https://doi.org/10.3389/fpls.2022.1025951 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fpls.2022.1025951 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 14 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 13 es_ES
dc.identifier.eissn 1664-462X es_ES
dc.identifier.pmid 36388476 es_ES
dc.identifier.pmcid PMC9647125 es_ES
dc.relation.pasarela S\483738 es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder COMISION DE LAS COMUNIDADES EUROPEA es_ES
dc.contributor.funder MINISTERIO DE CIENCIA E INNOVACION es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
upv.costeAPC 3412,87 es_ES


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