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dc.contributor.author | García-Ferrús, M.![]() |
es_ES |
dc.contributor.author | González Pellicer, Ana![]() |
es_ES |
dc.contributor.author | Pina-Pérez, María Consuelo![]() |
es_ES |
dc.contributor.author | Ferrús Pérez, Mª Antonia![]() |
es_ES |
dc.date.accessioned | 2023-10-26T18:02:23Z | |
dc.date.available | 2023-10-26T18:02:23Z | |
dc.date.issued | 2022-03 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/198878 | |
dc.description.abstract | [EN] Vegetables are one of the main foodstuffs consumed in the Mediterranean diet. However, raw vegetables have been associated with relevant foodborne outbreaks worldwide. Accurate knowledge of the microbiological quantitative risks associated with these matrices is crucial in order to define effective control measures, avoiding the survival and dissemination of foodborne pathogens through the different food chain stages. The aim of the present study is the assessment of the prevalence of Helicobacter pylori (a unique carcinogenic biological agent recognized to date) on leafy vegetables (spinach, lettuce, and chard) by means of the detection of the specific pathogenicity vacA gene. A real-time quantitative polymerase chain reaction (qPCR) optimized approach was used to detect H. pylori-positive samples and the concentration of this pathogen (with a limit of detection equal to 10 cells). One hundred raw vegetable samples were acquired in markets corresponding to the Spanish Mediterranean area. Sliced vegetable leaves were homogenized and centrifuged, and DNA was extracted from the homogenates. qPCR results confirmed 20 out of 100 H. pylori-positive samples, with melting temperature (T-m) values in the range of 84.8-86.5 degrees C (T-m vacA H. pylori = 85 degrees C). Amplicons were cut, purified, and sequenced to confirm the homology with the H. pylori vacA gene. A total of 17 out of 100 vegetable samples (12/45 (26.6%) lettuce, 2/21 (9.5%) spinach, and 3/34 (8.8%) chard samples) were finally confirmed as H. pylori-positive. Contamination levels were in the range of 1.5 +/- 0.3 to 2.5 +/- 0.1 log(10) cycles (36-335 CFU/g leafy vegetables). Our results show that H. pylori is detected by qPCR at levels close to infectious doses in fresh vegetables, thus posing a food safety hazard. | es_ES |
dc.description.sponsorship | This research was funded by the Ministerio de Ciencia e Innovacion, Spain, grant number PID2019-105691RB-I00. M. Garcia-Ferrus is the recipient of a PEJ2018-003746A grant from Ministerio de Economia, Industria y Competitividad, Spain. Prof. Pina-Perez is also grateful to the GVA202/031 grant project. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.relation.ispartof | Agriculture | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Helicobacter pylori | es_ES |
dc.subject | Carcinogenic biological agent | es_ES |
dc.subject | QPCR | es_ES |
dc.subject | Vegetables | es_ES |
dc.subject | Food safety | es_ES |
dc.subject.classification | MICROBIOLOGIA | es_ES |
dc.title | Helicobacter pylori Is Present at Quantifiable Levels in Raw Vegetables in the Mediterranean Area of Spain | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/agriculture12030339 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105691RB-I00/ES/ANALISIS INTEGRADO DE LA DIVERSIDAD, CALIDAD Y SEGURIDAD MICROBIOLOGICA DE ALIMENTOS VEGETALES ECOLOGICOS MEDIANTE CULTIVO, TECNICAS MOLECULARES Y SECUENCIACION MASIVA/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI//PEJ2018-003746-A-AR//AYUDA GARANTIA JUVENIL AEI. ACTUACION: EVALUACION DEL RIESGO PARA EL CONSUMIDOR POR LA PRESENCIA DE H. PYLORI Y OTROS HELICOBACTERS PATOGENOS EN ALIMENTOS, MEDIANTE HERRAMIENAS MOLECULARES Y DE METAGENOMICA/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//GVA%2F2020%2F031/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural | es_ES |
dc.description.bibliographicCitation | García-Ferrús, M.; González Pellicer, A.; Pina-Pérez, MC.; Ferrús Pérez, MA. (2022). Helicobacter pylori Is Present at Quantifiable Levels in Raw Vegetables in the Mediterranean Area of Spain. Agriculture. 12(3):1-7. https://doi.org/10.3390/agriculture12030339 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/agriculture12030339 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 7 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 12 | es_ES |
dc.description.issue | 3 | es_ES |
dc.identifier.eissn | 2077-0472 | es_ES |
dc.relation.pasarela | S\455954 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | AGENCIA ESTATAL DE INVESTIGACION | es_ES |
dc.subject.ods | 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible | es_ES |
dc.subject.ods | 12.- Garantizar las pautas de consumo y de producción sostenibles | es_ES |
upv.costeAPC | 2200 | es_ES |