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Multiview motion tracking based on a cartesian robot to monitor Caenorhabditis elegans in standard Petri dishes

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Multiview motion tracking based on a cartesian robot to monitor Caenorhabditis elegans in standard Petri dishes

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dc.contributor.author Puchalt-Rodríguez, Joan Carles es_ES
dc.contributor.author González-Rojo, José F. es_ES
dc.contributor.author Gómez-Escribano, Ana Pilar es_ES
dc.contributor.author Vázquez-Manrique, Rafael P. es_ES
dc.contributor.author Sánchez Salmerón, Antonio José es_ES
dc.date.accessioned 2023-10-30T19:03:34Z
dc.date.available 2023-10-30T19:03:34Z
dc.date.issued 2022-02-02 es_ES
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://hdl.handle.net/10251/199017
dc.description.abstract [EN] Data from manual healthspan assays of the nematode Caenorhabditis elegans (C. elegans) can be complex to quantify. The first attempts to quantify motor performance were done manually, using the so-called thrashing or body bends assay. Some laboratories have automated these approaches using methods that help substantially to quantify these characteristic movements in small well plates. Even so, it is sometimes difficult to find differences in motor behaviour between strains, and/or between treated vs untreated worms. For this reason, we present here a new automated method that increases the resolution flexibility, in order to capture more movement details in large standard Petri dishes, in such way that those movements are less restricted. This method is based on a Cartesian robot, which enables high-resolution images capture in standard Petri dishes. Several cameras mounted strategically on the robot and working with different fields of view, capture the required C. elegans visual information. We have performed a locomotion-based healthspan experiment with several mutant strains, and we have been able to detect statistically significant differences between two strains that show very similar movement patterns. es_ES
dc.description.sponsorship This work was supported by the research agency of the Spanish Ministry of Science and Innovation under Grant RTI2018-094312-B-I00 (European FEDER funds). es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Healthspan es_ES
dc.subject C. elegans es_ES
dc.subject Tracking es_ES
dc.subject Standard Petri dishes es_ES
dc.subject Cartesian robot es_ES
dc.subject Automated es_ES
dc.subject Multiview es_ES
dc.subject.classification INGENIERIA DE SISTEMAS Y AUTOMATICA es_ES
dc.title Multiview motion tracking based on a cartesian robot to monitor Caenorhabditis elegans in standard Petri dishes es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s41598-022-05823-6 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094312-B-I00/ES/MONITORIZACION AVANZADA DE COMPORTAMIENTOS DE CAENORHABDITIS ELEGANS, BASADA EN VISION ACTIVA, PARA ANALIZAR FUNCION COGNITIVA Y ENVEJECIMIENTO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.description.bibliographicCitation Puchalt-Rodríguez, JC.; González-Rojo, JF.; Gómez-Escribano, AP.; Vázquez-Manrique, RP.; Sánchez Salmerón, AJ. (2022). Multiview motion tracking based on a cartesian robot to monitor Caenorhabditis elegans in standard Petri dishes. Scientific Reports. 12(1):1-11. https://doi.org/10.1038/s41598-022-05823-6 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s41598-022-05823-6 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 11 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 12 es_ES
dc.description.issue 1 es_ES
dc.identifier.pmid 35110654 es_ES
dc.identifier.pmcid PMC8810772 es_ES
dc.relation.pasarela S\458816 es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder European Regional Development Fund es_ES
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dc.subject.ods 03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades es_ES
upv.costeAPC 1790 es_ES


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