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The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathway

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The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathway

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dc.contributor.author Wang, Fan es_ES
dc.contributor.author Zhang, Xiyu es_ES
dc.contributor.author Dai, Yingying es_ES
dc.contributor.author Zhao, Bohao es_ES
dc.contributor.author Wu, Xinsheng es_ES
dc.contributor.author Chen, Yang es_ES
dc.date.accessioned 2023-11-06T09:02:53Z
dc.date.available 2023-11-06T09:02:53Z
dc.date.issued 2023-09-28
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/199238
dc.description.abstract [EN] To explore the mechanism of the FRZB gene in hair follicle development by regulating the Wnt/β-catenin signalling pathway, Angora rabbits were selected to collect back skin samples for the experiment. The action mechanism is understood by cell culture and transfection, apoptosis and proliferation assays and TOP/FOP Flash Wnt Reporting System methods. The results showed that the interference and overexpression of the FRZB gene in rabbit dermal papilla cells indicated that overexpression could inhibit the expression of SFRP2, BMP4, and WNT2 genes (P<0.05). On the contrary, the expression of Wnt signalling pathway-related genes LEF1, CCND1, DKK1, and TCF7 was significantly up-regulated (P<0.05). Further examination of the luciferase reporter system TOP/FOP revealed that pcDNA3.1-FRZB inhibits Wnt activity. PcDNA3.1-FRZB was found to promote the level of apoptosis in DP cells, whereas si-FRZB inhibited DP cell proliferation. Therefore, it is concluded that FRZB inhibits hair follicle development in long-haired rabbits by regulating the Wnt/β-catenin signalling pathway. es_ES
dc.description.sponsorship Our study was funded by the National Natural Science Foundation of China (Grant No. 32072724), the Modern Agricultural Industrial System Special Funding (CARS-43-A-1) and the Zhejiang Science and Technology Major Programme on Agricultural New Variety Breeding (2021C02068-7). es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.relation.ispartof World Rabbit Science es_ES
dc.rights Reconocimiento - No comercial - Compartir igual (by-nc-sa) es_ES
dc.subject FRZB gene es_ES
dc.subject Hair follicle es_ES
dc.subject Long-haired rabbit es_ES
dc.title The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathway es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.4995/wrs.2023.18171
dc.relation.projectID info:eu-repo/grantAgreement/NSFC//32072724 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Science and Technology Department of Zhejiang Province//2021C02068-7 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Modern Agricultural Technology Industry System of Shandong province/CARS-43-A-1 es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Wang, F.; Zhang, X.; Dai, Y.; Zhao, B.; Wu, X.; Chen, Y. (2023). The FRZB gene regulates hair follicle development in rabbits via the Wnt/B-catenin signaling pathway. World Rabbit Science. 31(3):171-178. https://doi.org/10.4995/wrs.2023.18171 es_ES
dc.description.accrualMethod OJS es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2023.18171 es_ES
dc.description.upvformatpinicio 171 es_ES
dc.description.upvformatpfin 178 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 31 es_ES
dc.description.issue 3 es_ES
dc.identifier.eissn 1989-8886
dc.relation.pasarela OJS\18171 es_ES
dc.contributor.funder National Natural Science Foundation of China es_ES
dc.contributor.funder Science and Technology Department of Zhejiang Province es_ES
dc.contributor.funder Modern Agricultural Technology Industry System of Shandong province es_ES
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