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Cytotoxic and estrogenic activity of chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyrinidol. Study of marine yeasts as potential toxicity indicators

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Cytotoxic and estrogenic activity of chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyrinidol. Study of marine yeasts as potential toxicity indicators

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dc.contributor.author Echeverri-Jaramillo, Gustavo es_ES
dc.contributor.author Jaramillo-Colorado, Beatriz es_ES
dc.contributor.author Sabater Marco, Consuelo es_ES
dc.contributor.author Castillo López, María-Ángeles es_ES
dc.date.accessioned 2023-11-06T19:02:13Z
dc.date.available 2023-11-06T19:02:13Z
dc.date.issued 2021-01 es_ES
dc.identifier.issn 0963-9292 es_ES
dc.identifier.uri http://hdl.handle.net/10251/199370
dc.description.abstract [EN] Chlorpyrifos (CP) is one of the organophosphate insecticides most used worldwide today. Although the main target organ for CP is the nervous system triggering predominantly neurotoxic effects, it has suggested other mechanisms of action as cytotoxicity and endocrine disruption. The risk posed by the pesticide metabolites on non-target organisms is increasingly recognized by regulatory agencies and natural resource managers. In the present study, cytotoxicity and estrogenic activity of CP, and its principal metabolite 3,5,6-trichloro-2-pyridinol (TCP) have been evaluated by in vitro assays, using two mammalian cell lines (HEK293 and N2a), and a recombinant yeast. Results indicate that TCP is more toxic than CP for the two cell lines assayed, being N2a cells more sensitive to both compounds. Both compounds show a similar estrogenic activity being between 2500 and 3000 times less estrogenic than 17 beta-estradiol. In order to find new toxicity measurement models, yeasts isolated from marine sediments containing CP residues have been tested against CP and TCP by cell viability assay. Of the 12 yeast strains tested, 6 of them showed certain sensitivity, and a concentration-dependent response to the tested compounds, so they could be considered as future models for toxicity tests, although further investigations and proves are necessary. es_ES
dc.description.sponsorship The authors thank the Ecotoxicology Laboratory at the Department of Biotechnology of the School of Agricultural Engineering and Natural Environment (ETSIAMN) of the Polytechnic University of Valencia (Spain), and Universidad de San Buenaventura Cartagena and Universidad de Cartagena (Colombia). es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Ecotoxicology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cell viability es_ES
dc.subject Endocrine disruption es_ES
dc.subject Chlorpyrifos es_ES
dc.subject 3,5,6-trichloro-2-pyridinol es_ES
dc.subject Marine yeasts es_ES
dc.subject Bioassays es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title Cytotoxic and estrogenic activity of chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyrinidol. Study of marine yeasts as potential toxicity indicators es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10646-020-02315-z es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Echeverri-Jaramillo, G.; Jaramillo-Colorado, B.; Sabater Marco, C.; Castillo López, M. (2021). Cytotoxic and estrogenic activity of chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyrinidol. Study of marine yeasts as potential toxicity indicators. Ecotoxicology. 30(1):104-117. https://doi.org/10.1007/s10646-020-02315-z es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s10646-020-02315-z es_ES
dc.description.upvformatpinicio 104 es_ES
dc.description.upvformatpfin 117 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 30 es_ES
dc.description.issue 1 es_ES
dc.identifier.pmid 33249537 es_ES
dc.relation.pasarela S\423788 es_ES
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