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Molecular analysis of a Spanish isolate of chili pepper mild mottle virus and evaluation of seed transmission and resistance genes

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Molecular analysis of a Spanish isolate of chili pepper mild mottle virus and evaluation of seed transmission and resistance genes

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dc.contributor.author Ontañon, Clara es_ES
dc.contributor.author Ojinaga, Mikel es_ES
dc.contributor.author Larregla, Santiago es_ES
dc.contributor.author Zabala, Joseba Andoni es_ES
dc.contributor.author Reva, Anastasiia es_ES
dc.contributor.author Losa, Alba es_ES
dc.contributor.author Heribia, Rosario es_ES
dc.contributor.author Bertacca, Sofia es_ES
dc.contributor.author Sanahuja, Esmeralda es_ES
dc.contributor.author Alfaro Fernández, Ana Olvido es_ES
dc.contributor.author Font San Ambrosio, Maria Isabel es_ES
dc.contributor.author Corachán Valencia, Lorena es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.contributor.author SANCHEZ NAVARRO, JESUS ANGEL es_ES
dc.date.accessioned 2023-12-21T19:01:43Z
dc.date.available 2023-12-21T19:01:43Z
dc.date.issued 2023-09-18 es_ES
dc.identifier.issn 0929-1873 es_ES
dc.identifier.uri http://hdl.handle.net/10251/201039
dc.description.abstract [EN] An isolate of chili pepper mild mottle virus (CPMMV-Sp; GenBank OQ920979) with a 99% identity to CPMMV (GenBank MN164455.1) was found in symptomatic pepper plants in Spain. RACE analysis, performed using a stem-loop primer developed in this study to prime at the end of the introduced poly(A)/(U) tail, revealed the presence of an extra 22 nt at the 5' end, starting with a cytosine, which were essential to generate infectious clones. However, the 5' terminal cytosine was dispensable for initiating the infection. The design of two specific digoxigenin riboprobes targeting the more divergent area of CPMMV-Sp, compared to the closely related bell pepper mottle virus (BPeMV) (identity percentage of 80.6% and 75.8%, respectively), showed that both probes specifically detected CPMMV-Sp when the hybridization was performed at 68oC and 60oC, respectively. However, the BPeMV probe, targeting a region with an 89.4% identity percentage to CPMMV-Sp, showed cross-hybridization at 60oC but not at 68oC. The comparison of the detection limits between molecular hybridization and RT-PCR techniques revealed that the former was 125 times less sensitive than RT-PCR. The analysis of the vertical transmission of CPMMV-Sp using seeds from naturally or mechanically infected pepper plants revealed a transmission percentage ranging from 0.9% to 8.5%. Finally, the analysis of the resistance of capsicum species carrying different alleles of the L gene (L1, L2, L3, and L4) revealed that varieties with the L1 gene were infected by CPMMV-Sp (20-40% of inoculated plants), while varieties with the L2, L3, and L4 genes were resistant. es_ES
dc.description.sponsorship This work was supported by grants PID2020-115571RB-100 and TED2021-131949B-I00 from the Spanish Agencia Estatal de Investigacion (AEI) and Fondo Europeo de Desarrollo Regional (FEDER). Project 20-00032-VIRUSPIM from Dept. of Environment, Territorial Planning, Agriculture and Fisheries (Basque Government). Mikel Ojinaga was the recipient of a PhD contract "Introduction of Resistance to Tobamovirus and other Viruses in Landraces of Gernika Pepper and Ibarra Chili Pepper" (Order of 24 October 2018 of the Minister of Economic Development and Competitiveness of the Basque Government). Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof European Journal of Plant Pathology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Tobamovirus es_ES
dc.subject Non-radioactive molecular hybridization es_ES
dc.subject Seed transmission es_ES
dc.subject Infectious clones es_ES
dc.subject L resistance genes es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.title Molecular analysis of a Spanish isolate of chili pepper mild mottle virus and evaluation of seed transmission and resistance genes es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10658-023-02765-1 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115571RB-I00/ES/LA MODIFICACION N6-METILADENOSINA (M6A) DEL RNA Y EL DIRECCIONAMIENTO DUAL A CLOROPLASTOS Y MITOCONDRIAS COMO MECANISMOS REGULADORES EN LA BIOLOGIA DE LOS VIRUS RNA DE PLANTAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Eusko Jaurlaritza//20-00032-VIRUSPIM/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//TED2021-131949B-I00/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Ontañon, C.; Ojinaga, M.; Larregla, S.; Zabala, JA.; Reva, A.; Losa, A.; Heribia, R.... (2023). Molecular analysis of a Spanish isolate of chili pepper mild mottle virus and evaluation of seed transmission and resistance genes. European Journal of Plant Pathology. 1-18. https://doi.org/10.1007/s10658-023-02765-1 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s10658-023-02765-1 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 18 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.relation.pasarela S\505626 es_ES
dc.contributor.funder Eusko Jaurlaritza es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
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