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dc.contributor.author | Fernández Blanco, Ana![]() |
es_ES |
dc.contributor.author | Hernández Pérez, Manuel![]() |
es_ES |
dc.contributor.author | Moreno Trigos, Mª Yolanda![]() |
es_ES |
dc.contributor.author | García Hernández, Jorge![]() |
es_ES |
dc.date.accessioned | 2024-03-04T19:02:47Z | |
dc.date.available | 2024-03-04T19:02:47Z | |
dc.date.issued | 2023-06 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/202919 | |
dc.description.abstract | [EN] The present work describes an alternative method for detecting and identifying Listeria monocytogenes in food samples by developing a nanophotonic biosensor containing bioreceptors and optical transducers. The development of photonic sensors for the detection of pathogens in the food industry involves the implementation of procedures for selecting probes against the antigens of interest and the functionalization of the sensor surfaces on which the said bioreceptors are located. As a previous step to functionalizing the biosensor, an immobilization control of these antibodies on silicon nitride surfaces was carried out to check the effectiveness of in plane immobilization. On the one hand, it was observed that a Listeria monocytogenes-specific polyclonal antibody has a greater binding capacity to the antigen at a wide range of concentrations. A Listeria monocytogenes monoclonal antibody is more specific and has a greater binding capacity only at low concentrations. An assay for evaluating selected antibodies against particular antigens of Listeria monocytogenes bacteria was designed to determine the binding specificity of each probe using the indirect ELISA detection technique. In addition, a validation method was established against the reference method for many replicates belonging to different batches of meat-detectable samples, with a medium and pre-enrichment time that allowed optimal recovery of the target microorganism. Moreover, no cross-reactivity with other nontarget bacteria was observed. Thus, this system is a simple, highly sensitive, and accurate platform for L. monocytogenes detection. | es_ES |
dc.description.sponsorship | This research was funded by AVI (Valencian Innovation Agency) and the European Union through FEDER founds, INNEST/2021/338, BACTERIO project ¿Integrated Photonics for integral microbiological control in the agri-food sector¿. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.relation.ispartof | Sensors | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Listeria monocytogenes | es_ES |
dc.subject | Food safety | es_ES |
dc.subject | Biosensor | es_ES |
dc.subject | Nanophotonics | es_ES |
dc.subject | Bioreceptor | es_ES |
dc.subject | Optical transducers | es_ES |
dc.subject.classification | MICROBIOLOGIA | es_ES |
dc.title | Development of Optical label-free Biosensor method in Detection of Listeria monocytogenes from Food | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/s23125570 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement///INNEST%2F2021%2F338// Fotónica integrada para el control microbiológico integral en el sector agroalimentario/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural | es_ES |
dc.description.bibliographicCitation | Fernández Blanco, A.; Hernández Pérez, M.; Moreno Trigos, MY.; García Hernández, J. (2023). Development of Optical label-free Biosensor method in Detection of Listeria monocytogenes from Food. Sensors. 23(12). https://doi.org/10.3390/s23125570 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/s23125570 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 23 | es_ES |
dc.description.issue | 12 | es_ES |
dc.identifier.eissn | 1424-8220 | es_ES |
dc.identifier.pmid | 37420736 | es_ES |
dc.identifier.pmcid | PMC10301194 | es_ES |
dc.relation.pasarela | S\495042 | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Agència Valenciana de la Innovació | es_ES |
dc.subject.ods | 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible | es_ES |
dc.subject.ods | 03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades | es_ES |