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Analysis of Exosomal Cargo Provides Accurate Clinical, Histologic and Mutational Information in Non-Small Cell Lung Cancer

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Analysis of Exosomal Cargo Provides Accurate Clinical, Histologic and Mutational Information in Non-Small Cell Lung Cancer

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dc.contributor.author Duréndez-Sáez, Elena es_ES
dc.contributor.author Calabuig-Fariñas, Silvia es_ES
dc.contributor.author Torres-Martínez, Susana es_ES
dc.contributor.author Moreno-Manuel, Andrea es_ES
dc.contributor.author Herreros-Pomares, Alejandro es_ES
dc.contributor.author Escorihuela, Eva es_ES
dc.contributor.author Mosqueda, Marais es_ES
dc.contributor.author Gallach, Sandra es_ES
dc.contributor.author Guijarro, Ricardo es_ES
dc.contributor.author Serna, Eva es_ES
dc.contributor.author Suárez-Cabrera, Cristian es_ES
dc.contributor.author Paramio, Jesús M. es_ES
dc.contributor.author Blasco, Ana es_ES
dc.contributor.author Camps, Carlos es_ES
dc.contributor.author Jantus-Lewintre, Eloisa es_ES
dc.date.accessioned 2024-04-29T18:08:43Z
dc.date.available 2024-04-29T18:08:43Z
dc.date.issued 2022-07 es_ES
dc.identifier.uri http://hdl.handle.net/10251/203855
dc.description.abstract [EN] Lung cancer is a malignant disease with high mortality and poor prognosis, frequently diagnosed at advanced stages. Nowadays, immense progress in treatment has been achieved. However, the present scenario continues to be critical, and a full comprehension of tumor progression mechanisms is required, with exosomes being potentially relevant players. Exosomes are membranous vesicles that contain biological information, which can be transported cell-to-cell and modulate relevant processes in the hallmarks of cancer. The present research aims to characterize the exosomes' cargo and study their role in NSCLC to identify biomarkers. We analyzed exosomes secreted by primary cultures and cell lines, grown in monolayer and tumorsphere formations. Exosomal DNA content showed molecular alterations, whereas RNA high-throughput analysis resulted in a pattern of differentially expressed genes depending on histology. The most significant differences were found in XAGE1B, CABYR, NKX2-1, SEPP1, CAPRIN1, and RIOK3 genes when samples from two independent cohorts of resected NSCLC patients were analyzed. We identified and validated biomarkers for adenocarcinoma and squamous cell carcinoma. Our results could represent a relevant contribution concerning exosomes in clinical practice, allowing for the identification of biomarkers that provide information regarding tumor features, prognosis and clinical behavior of the disease. es_ES
dc.description.sponsorship This research was founded by Centro de Investigacion Biomedica en Red de Cancer (CIBERONC) (grant number CB16-12-00350), Instituto de Salud Carlos III (PI18/00266), and Generalitat Valenciana (GV/2018/026). The authors E.D.-S. and A.M.-M. have a predoctoral fellowship (PRDVA18015MORE) from the Scientific Foundation of Asociación Española Contra el Cáncer, Valencia (AECC Valencia). A.H.-P. is supported by Ayudas Margarita Salas from Ministerio de Universidades, Unión EuropeaNext generation EU. S.T.-M. is supported by the Generalitat Valenciana and Fondo Social Europeo fellowship (ACIF/2018/275). es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Cancers es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Non-small cell lung cancer es_ES
dc.subject Liquid biopsy es_ES
dc.subject Exosomes es_ES
dc.subject Extracellular vesicles es_ES
dc.subject Cell cultures es_ES
dc.subject Adenocarcinoma es_ES
dc.subject Squamous cell carcinoma es_ES
dc.subject Biomarker es_ES
dc.subject Tumorspheres es_ES
dc.subject.classification BIOLOGIA CELULAR es_ES
dc.title Analysis of Exosomal Cargo Provides Accurate Clinical, Histologic and Mutational Information in Non-Small Cell Lung Cancer es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/cancers14133216 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 (ISCIII)/PI18%2F00266/ES/INMUNOGRAMA NO INVASIVO. APROXIMACION MULTIDIMENSIONAL PARA CARACTERIZAR Y MONITORIZAR EL ESTATUS INMUNE EN CANCER DE PULMON./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//GV%2F2018%2F026/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACIF%2F2018%2F275/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AECC//PRDVA18015MORE/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CIBERONC//CB16-12-00350/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Duréndez-Sáez, E.; Calabuig-Fariñas, S.; Torres-Martínez, S.; Moreno-Manuel, A.; Herreros-Pomares, A.; Escorihuela, E.; Mosqueda, M.... (2022). Analysis of Exosomal Cargo Provides Accurate Clinical, Histologic and Mutational Information in Non-Small Cell Lung Cancer. Cancers. 14(13). https://doi.org/10.3390/cancers14133216 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/cancers14133216 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 14 es_ES
dc.description.issue 13 es_ES
dc.identifier.eissn 2072-6694 es_ES
dc.identifier.pmid 35804987 es_ES
dc.identifier.pmcid PMC9264915 es_ES
dc.relation.pasarela S\469417 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder Asociación Española Contra el Cáncer es_ES
dc.contributor.funder Centro de Investigación Biomédica en Red de Cáncer es_ES


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