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dc.contributor.author | González-Cebrián, Alba![]() |
es_ES |
dc.contributor.author | Borràs-Ferrís, Joan![]() |
es_ES |
dc.contributor.author | Boada-Acosta, Yadira Fernanda![]() |
es_ES |
dc.contributor.author | Vignoni, Alejandro![]() |
es_ES |
dc.contributor.author | Ferrer, Alberto![]() |
es_ES |
dc.contributor.author | Picó, Jesús![]() |
es_ES |
dc.date.accessioned | 2024-05-14T18:03:36Z | |
dc.date.available | 2024-05-14T18:03:36Z | |
dc.date.issued | 2023-01-20 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/204157 | |
dc.description.abstract | [EN] One of the most common sources of information in Synthetic Biology is the data coming from plate reader fluorescence measurements. These experiments provide a measure of the light emitted by a certain fluorescent molecule, such as the Green Fluorescent Protein (GFP). However, these measurements are generally expressed in arbitrary units and are affected by the measurement device gain. This limits the range of measurements in a single experiment and hampers the comparison of results among experiments. In this work, we describe PLATERO, a calibration protocol to express fluorescence measures in concentration units of a reference fluorophore. The protocol removes the gain effect of the measurement device on the acquired data. In addition, the fluorescence intensity values are transformed into units of concentration using a Fluorescein calibration model. Both steps are expressed in a single mathematical expression that returns normalized, gain-independent, and comparable data, even if the acquisition was done at different device gain levels. Most important, the PLATERO embeds a Linearity and Bias Analysis that provides an assessment of the uncertainty of the model estimations, and a Reproducibility and Repeatability analysis that evaluates the sources of variability originating from the measurements and the equipment. All the functions used to build the model, exploit it with new data, and perform the uncertainty and variability assessment are available in an open access repository. | es_ES |
dc.description.sponsorship | This research was funded by MCIN/AEI/10.13039/501100011033 grant numbers PID2020-117271RB-C21 and PID2020-119262RB-I00, Generalitat Valenciana grants CIAICO/2021/159 and CIAICO/2021/111, and Universitat Politècnica de València grants PAID-01-17 (AG-C) and PAID10-21 (YB). YB thanks to Secretaría de Educación Superior, Ciencia, Tecnología e Innovación of Ecuador (Scholarship Convocatoria Abierta 2011). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Frontiers Media S.A. | es_ES |
dc.relation.ispartof | Frontiers in Bioengineering and Biotechnology | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Fluorescence measurements | es_ES |
dc.subject | Measurement data standardization | es_ES |
dc.subject | Measurement system analysis | es_ES |
dc.subject | Plate reader | es_ES |
dc.subject | Units conversion model | es_ES |
dc.subject.classification | INGENIERIA DE SISTEMAS Y AUTOMATICA | es_ES |
dc.subject.classification | ESTADISTICA E INVESTIGACION OPERATIVA | es_ES |
dc.title | PLATERO: A Calibration Protocol for Plate Reader Green Fluorescence Measurements | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3389/fbioe.2023.1104445 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-117271RB-C21/ES/REGULACION DINAMICA MULTI-ESCALA EN INGENIERIA METABOLICA: CONTROL REALIMENTADO Y CICLO DBTL AUTOMATIZADO / | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-119262RB-I00/ES/TECNICAS ESTADISTICAS MULTIVARIANTES BASADAS EN VARIABLES LATENTES PARA EL DESARROLLO DE BIOMARCADORES DE IMAGEN PARA LA DIAGNOSIS Y PROGNOSIS DE CANCER DE MAMA/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UPV//PAID-01-17/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//CIAICO%2F2021%2F111/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería del Diseño - Escola Tècnica Superior d'Enginyeria del Disseny | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials | es_ES |
dc.description.bibliographicCitation | González-Cebrián, A.; Borràs-Ferrís, J.; Boada-Acosta, YF.; Vignoni, A.; Ferrer, A.; Picó, J. (2023). PLATERO: A Calibration Protocol for Plate Reader Green Fluorescence Measurements. Frontiers in Bioengineering and Biotechnology. 11. https://doi.org/10.3389/fbioe.2023.1104445 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3389/fbioe.2023.1104445 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 11 | es_ES |
dc.identifier.eissn | 2296-4185 | es_ES |
dc.identifier.pmid | 36741754 | es_ES |
dc.identifier.pmcid | PMC9895789 | es_ES |
dc.relation.pasarela | S\481172 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | AGENCIA ESTATAL DE INVESTIGACION | es_ES |
dc.contributor.funder | Universitat Politècnica de València | es_ES |
dc.contributor.funder | Secretaría de Educación Superior, Ciencia, Tecnología e Innovación, Ecuador | es_ES |