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Characterization of potential spermatogonia biomarker genes in the European eel (Anguilla anguilla)

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Characterization of potential spermatogonia biomarker genes in the European eel (Anguilla anguilla)

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dc.contributor.author Blanes-García, Marta es_ES
dc.contributor.author Marinovic, Zoran es_ES
dc.contributor.author Herranz-Jusdado, Juan Germán es_ES
dc.contributor.author Xie, Xuan es_ES
dc.contributor.author Ferrao, Leonor es_ES
dc.contributor.author Gallego Albiach, Victor es_ES
dc.contributor.author Pérez Igualada, Luz María es_ES
dc.contributor.author Horváth, Ákos es_ES
dc.contributor.author Psenicka, Martin es_ES
dc.contributor.author Asturiano, Juan F. es_ES
dc.contributor.author Morini, Marina es_ES
dc.contributor.author Baloch, Abdul Rasheed
dc.date.accessioned 2024-07-22T18:05:37Z
dc.date.available 2024-07-22T18:05:37Z
dc.date.issued 2024-04-19 es_ES
dc.identifier.issn 0920-1742 es_ES
dc.identifier.uri http://hdl.handle.net/10251/206520
dc.description.abstract [EN] Identification of specific molecular markers for spermatogonial stem cells in teleost is crucial for enhancing the efficacy of reproductive biotechnologies in aquaculture, such as transplantation and surrogate production in fishes. Since it is not yet possible to distinguish spermatogonial stem cells of European eel (Anguilla anguilla) using specific molecular markers, we isolated spermatogonial cells from immature European eels to find these potential markers. We attempted this by studying three candidate genes: vasa, nanos2, and dnd1. Two vasa (vasa1 and vasa2) genes, nanos2, and dnd1 were identified, characterized, and studied in the muscle, testis, and isolated spermatogonia. Our results showed that vasa1 and vasa2 had the highest levels of expression when measured by qPCR. In situ hybridization and immunochemistry assays showed that the four genes were localized explicitly in type A spermatogonia. However, vasa1 and vasa2 exhibited stronger signals in the immature testicular tissue than the other two potential markers. According to this, vasa1 and vasa2 were found to be the most effective markers for spermatogonial cells in the European eel. es_ES
dc.description.sponsorship Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Funded by the European Union s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement Nº 642893 (IMPRESS; including the JGHJ and ARB predoctoral contracts), and by the Ministerio de Ciencia e Innovación y Universidades (Project EELGONIA; RTI2018-096413-BI00). MBG and LF have PhD contracts from the UPV (PAID01 20) and GVA (GRISOLIAP/2020/063), respectively. VG joined a grant RYC2021-031558-I funded by MCIN/AEI/10.13039/501100011033, and by European Union NextGenerationEU/PRTR. MM has a postdoc contract supported byMCIN/AEI/10.13039/501100011033 funded by European Union NextGenerationEU/PRTR (C17.I1) and by Generalitat Valenciana (THINKINAZUL/2021/012) to SEASPERM. MP was fnancially supported by the Ministry of Education, Youth and Sports of the Czech Republic project CENAKVA (LM2018099) and Biodiversity (CZ.02.1.01/0.0/0.0/16_025/0007370), and the Czech Science Foundation (grant number 22-31141 J)). ZM and ÁH were supported by the Ministry of Innovation and Technology within the framework of the Thematic Excellence Programme 2020, National Challenges Subprogramme (TKP2020-NKA-16) as well as the NKFIH K138425 and FK 142933 projects. Funding for open access charge: CRUE-Universitat Politècnica de València. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Fish Physiology and Biochemistry es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Testis es_ES
dc.subject Fish es_ES
dc.subject Gene expression es_ES
dc.subject Vasa es_ES
dc.subject Dnd1 es_ES
dc.subject Nanos2 es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Characterization of potential spermatogonia biomarker genes in the European eel (Anguilla anguilla) es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10695-024-01338-1 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096413-B-I00/ES/DESARROLLO Y APLICACION DE LAS TECNICAS DE PRODUCCION SUBROGADA DE GAMETOS DE ANGUILA EUROPEA MEDIANTE XENOTRASPLANTE DE ESPERMATOGONIAS EN OTRAS ESPECIES DE PECES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/642893/EU/Improved production strategies for endangered freshwater species./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-01-20/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//GRISOLIAP%2F2020%2F063/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//THINKINAZUL%2F2021%2F012/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MEYS//LM2018099/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GACR//22-31141 J/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RYC2021-031558-I/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//PRTR-C17.I1/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ITM//TKP2020- NKA-16/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ITM//NKFIH K138425/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ITM//FK 142933/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Blanes-García, M.; Marinovic, Z.; Herranz-Jusdado, JG.; Xie, X.; Ferrao, L.; Gallego Albiach, V.; Pérez Igualada, LM.... (2024). Characterization of potential spermatogonia biomarker genes in the European eel (Anguilla anguilla). Fish Physiology and Biochemistry. https://doi.org/10.1007/s10695-024-01338-1 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s10695-024-01338-1 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.relation.pasarela S\522405 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Grant Agency of the Czech Republic es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.contributor.funder Ministry of Innovation and Technology, Hungría es_ES
dc.contributor.funder Ministry of Education, Youth and Sport of the Czech Republic es_ES


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