Milkani, E.; Khaing, A.; Morais Ezquerro, SB.; C.R. LAMBERT; W.G. MCGIMPSEY (2011). SPR-based single nucleotide mismatch biosensor. Analytical Methods. 3:122-132. doi:10.1039/c0ay00492h
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/27814
Title:
|
SPR-based single nucleotide mismatch biosensor
|
Author:
|
Milkani, E.
Khaing, A.M.
Morais Ezquerro, Sergi Beñat
C.R. LAMBERT
W.G. MCGIMPSEY
|
UPV Unit:
|
Universitat Politècnica de València. Departamento de Química - Departament de Química
|
Issued date:
|
|
Abstract:
|
The detection and characterization of the hybridization event of 21-base, unlabeled DNA oligonucleotides with a monolayer of complementary DNA immobilized on a gold surface, by electrochemical impedance spectroscopy and ...[+]
The detection and characterization of the hybridization event of 21-base, unlabeled DNA oligonucleotides with a monolayer of complementary DNA immobilized on a gold surface, by electrochemical impedance spectroscopy and surface plasmon resonance (SPR) is presented. A thiol modification on the probe DNA strand allowed for its attachment to the surface via self-assembly. For the hybridization of full match target DNA a detection limit of 20 pM was determined. RNA hybridization was also detectable with the same sensor, with a similar detection limit. The SPR signal generated upon hybridization of the full match was always distinguishable from the single mismatch target DNA oligonucleotides when the mismatch was in the middle or at the proximal end of the target DNA sequence. However, the response of the sensor was identical for the hybridization of the full match and the distal end mismatch. The SPR sensor described is reusable over at least 20 hybridization/regeneration cycles and is insensitive to flow rate (20-800 L min -1) or temperature (20-60°C). Based on the SPR response, the surface density of the probe was estimated to be at least 4.3 ¿ 10 12 molecules per cm 2. © 2011 The Royal Society of Chemistry.
[-]
|
Subjects:
|
Detection limits
,
DNA oligonucleotides
,
DNA strands
,
Gold surfaces
,
RNA hybridization
,
Single nucleotides
,
SPR sensors
,
SPR signals
,
Surface density
,
Biosensors
,
DNA
,
Electrochemical corrosion
,
Electrochemical impedance spectroscopy
,
Monolayers
,
Nucleic acids
,
Oligonucleotides
,
Probes
,
RNA
,
Surface plasmon resonance
|
Copyrigths:
|
Cerrado |
Source:
|
Analytical Methods. (issn:
1759-9660
)
|
DOI:
|
10.1039/c0ay00492h
|
Publisher:
|
Royal Society of Chemistry
|
Publisher version:
|
http://dx.doi.org/10.1039/c0ay00492h
|
Thanks:
|
This project was partially supported by the US Army Medical Research and Materiel Command (USAMRMC) and the Telemedicine and Advanced Technology Research Center (TATRC) We note that ICx Nomadics (Stillwater, OK, USA) is ...[+]
This project was partially supported by the US Army Medical Research and Materiel Command (USAMRMC) and the Telemedicine and Advanced Technology Research Center (TATRC) We note that ICx Nomadics (Stillwater, OK, USA) is now ICx Technologies (Arlington, VA, USA).
[-]
|
Type:
|
Artículo
|