- -

Heat Treatment Effects on the Antimicrobial Activity of Macrolide and Lincosamide Antibiotics in Milk

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Heat Treatment Effects on the Antimicrobial Activity of Macrolide and Lincosamide Antibiotics in Milk

Show simple item record

Files in this item

dc.contributor.author Zorraquino Lozano, Miguel Angel es_ES
dc.contributor.author Althaus, R. L. es_ES
dc.contributor.author Roca Marugán, Marta Isabel es_ES
dc.contributor.author Molina Pons, Mª Pilar es_ES
dc.date.accessioned 2013-05-02T11:21:33Z
dc.date.issued 2011-02
dc.identifier.issn 0362-028X
dc.identifier.uri http://hdl.handle.net/10251/28390
dc.description.abstract [EN] Aims: In this study, a microbiological method of dichotomous response using Bacillus cereus was designed and optimized to detect tetracyclines (TCs) at concentrations near to the maximum residue limits (MRLs). Methods and Results: In a first stage, the response time of bioassay was reduced to 5h when the logarithm of spore concentration (log S) was increased. Later, a Plackett Burman design (2 6-3) was analysed using logistic regression model. This design indicates significant effects of log S and chloramphenicol (CAP) on the detection limit (DL) of TC. Then, the response surfaces (RS) of the TCs DTs as a function of log S and CAP were plotted using a Dohlert design and the logistic regression model. These RS show a linear decrease with the raise of CAP and a quadratic effect of log S. Finally, the DTs of TC (109¿gl -1) and oxytetracycline (100¿gl -1) were adjusted to their MRLs through the desirability function. Conclusions: By successive application of experimental design techniques could be optimized a bioassay for the detection of TC residues in milk. The best conditions have been achieved when the assay was made with log S=5·12 and CAP=470¿gl -1. Significance and Impact of the Study: Experimental design techniques together with the logistic regression model and the desirability function represent an adequate tool for the optimization of a bioassay with binary response. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology. es_ES
dc.description.sponsorship This research has been carried out as part of the AGL2003-03663 project and has been financially assisted by the Spanish Ministry of Education and Science (Madrid, Spain). The authors thank the Polytechnic University of Valencia (UPV, Spain) for funding Dr. Rafael Althaus's collaboration with the Institute for Animal Science and Technology (UPV). en_EN
dc.language Inglés es_ES
dc.publisher International Association for Food Protection es_ES
dc.relation MEC/AGL2003-03663 es_ES
dc.relation.ispartof Journal of Food Protection es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Bioassay es_ES
dc.subject Experimental design es_ES
dc.subject Milk es_ES
dc.subject Screening test es_ES
dc.subject Tetracycline es_ES
dc.subject Chloramphenicol es_ES
dc.subject Bacterium es_ES
dc.subject Logistics es_ES
dc.subject Optimization es_ES
dc.subject Regression analysis es_ES
dc.subject Article es_ES
dc.subject Bacillus cereus es_ES
dc.subject Bacterial spore es_ES
dc.subject Chemometric analysis es_ES
dc.subject Limit of detection es_ES
dc.subject Logistic regression analysis es_ES
dc.subject Nonhuman es_ES
dc.subject Animals es_ES
dc.subject Anti-Bacterial Agents es_ES
dc.subject Biological Assay es_ES
dc.subject Food Analysis es_ES
dc.subject Logistic Models es_ES
dc.subject Microbial Sensitivity Tests es_ES
dc.subject Sensitivity and Specificity es_ES
dc.subject Spores, Bacterial es_ES
dc.subject Tetracyclines es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Heat Treatment Effects on the Antimicrobial Activity of Macrolide and Lincosamide Antibiotics in Milk es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.4315/0362-028X.JFP-10-297
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Zorraquino Lozano, MÁ.; Althaus, RL.; Roca Marugán, MI.; Molina Pons, MP. (2011). Heat Treatment Effects on the Antimicrobial Activity of Macrolide and Lincosamide Antibiotics in Milk. Journal of Food Protection. 74(2):311-315. doi:10.4315/0362-028X.JFP-10-297 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.4315/0362-028X.JFP-10-297 es_ES
dc.description.upvformatpinicio 311 es_ES
dc.description.upvformatpfin 315 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 74 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 40915
dc.contributor.funder Ministerio de Educación y Ciencia
dc.contributor.funder Universitat Politècnica de València


This item appears in the following Collection(s)

Show simple item record