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High-throughput sequencing, characterization and detection of new and conserved cucumber miRNAs

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High-throughput sequencing, characterization and detection of new and conserved cucumber miRNAs

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dc.contributor.author Martínez Navarro, Germán es_ES
dc.contributor.author Forment Millet, José Javier es_ES
dc.contributor.author Llave, Cesar es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.contributor.author Gomez, Gustavo Germán es_ES
dc.date.accessioned 2013-05-06T13:38:27Z
dc.date.available 2013-05-06T13:38:27Z
dc.date.issued 2011
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10251/28589
dc.description.abstract [EN] Micro RNAS (miRNAs) are a class of endogenous small non coding RNAs involved in the post-transcriptional regulation of gene expression. In plants, a great number of conserved and specific miRNAs, mainly arising from model species, have been identified to date. However less is known about the diversity of these regulatory RNAs in vegetal species with agricultural and/or horticultural importance. Here we report a combined approach of bioinformatics prediction, high-throughput sequencing data and molecular methods to analyze miRNAs populations in cucumber (Cucumis sativus) plants. A set of 19 conserved and 6 known but non-conserved miRNA families were found in our cucumber small RNA dataset. We also identified 7 (3 with their miRNA* strand) not previously described miRNAs, candidates to be cucumber-specific. To validate their description these new C. sativus miRNAs were detected by northern blot hybridization. Additionally, potential targets for most conserved and new miRNAs were identified in cucumber genome. In summary, in this study we have identified, by first time, conserved, known non-conserved and new miRNAs arising from an agronomically important species such as C. sativus. The detection of this complex population of regulatory small RNAs suggests that similarly to that observe in other plant species, cucumber miRNAs may possibly play an important role in diverse biological and metabolic processes. es_ES
dc.description.sponsorship This work was supported by grant BIO2008-03528 from the Spanish granting agency DGICYT and by grant ACOMP/2010/214 from the Generalitat Valenciana. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS ONE es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Arabidopsis-Thaliana es_ES
dc.subject Plant micrornas es_ES
dc.subject Messenger-rna es_ES
dc.subject Genes es_ES
dc.subject Identification es_ES
dc.subject Evolution es_ES
dc.subject Targets es_ES
dc.subject Annotation es_ES
dc.subject Expression es_ES
dc.subject Cleavage es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title High-throughput sequencing, characterization and detection of new and conserved cucumber miRNAs es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.pone.0019523
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2008-03528/ES/INTERACCIONES PATOGENO-HUESPED EN EL TRANSPORTE CELULAR Y VASCULAR DE VIRUS DE INTERES AGRONOMICO./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACOMP%2F2010%2F214/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Martínez Navarro, G.; Forment Millet, JJ.; Llave, C.; Pallás Benet, V.; Gomez, GG. (2011). High-throughput sequencing, characterization and detection of new and conserved cucumber miRNAs. PLoS ONE. 5(6):19523-19523. https://doi.org/10.1371/journal.pone.0019523 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1371/journal.pone.0019523 es_ES
dc.description.upvformatpinicio 19523 es_ES
dc.description.upvformatpfin 19523 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 5 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 217469
dc.identifier.pmid 21603611 en_EN
dc.identifier.pmcid PMC3095615 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Generalitat Valenciana es_ES
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