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A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae

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A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae

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dc.contributor.author Barreto, Lina es_ES
dc.contributor.author Canadell, David es_ES
dc.contributor.author Petrezselyova, Silvia es_ES
dc.contributor.author Navarrete, Clara es_ES
dc.contributor.author Maresova, Lydie es_ES
dc.contributor.author Pérez Valle, Jorge es_ES
dc.contributor.author Herrera, Rito es_ES
dc.contributor.author Olier, Ivan es_ES
dc.contributor.author Giraldo, Jesus es_ES
dc.contributor.author Sychrova, Hana es_ES
dc.contributor.author Yenush, Lynne es_ES
dc.contributor.author Ramos, Jose es_ES
dc.contributor.author Arino, Joaquin
dc.date.accessioned 2013-06-11T12:22:58Z
dc.date.available 2013-06-11T12:22:58Z
dc.date.issued 2011
dc.identifier.issn 1535-9778
dc.identifier.uri http://hdl.handle.net/10251/29635
dc.description.abstract [EN] Potassium homeostasis is crucial for living cells. In the yeast Saccharomyces cerevisiae, the uptake of potassium is driven by the electrochemical gradient generated by the Pma1 H +-ATPase, and this process represents a major consumer of the gradient. We considered that any mutation resulting in an alteration of the electrochemical gradient could give rise to anomalous sensitivity to any cationic drug independently of its toxicity mechanism. Here, we describe a genomewide screen for mutants that present altered tolerance to hygromycin B, spermine, and tetramethylammonium. Two hundred twenty-six mutant strains displayed altered tolerance to all three drugs (202 hypersensitive and 24 hypertolerant), and more than 50% presented a strong or moderate growth defect at a limiting potassium concentration (1 mM). Functional groups such as protein kinases and phosphatases, intracellular trafficking, transcription, or cell cycle and DNA processing were enriched. Essentially, our screen has identified a substantial number of genes that were not previously described to play a direct or indirect role in potassium homeostasis. A subset of 27 representative mutants were selected and subjected to diverse biochemical tests that, in some cases, allowed us to postulate the basis for the observed phenotypes. © 2011, American Society for Microbiology. All Rights Reserved. es_ES
dc.description.sponsorship This work was supported by grants BFU2008-04188-C03-01, GEN2006-27748-C2-1-E/SYS (SysMo ERA-NET), and EUI200904147 (SysMo2 ERA-NET) to J.A.; GEN2006-27748-C2-2-E/SYS (SysMo ERA-NET) and BFU2008-04188-C03-03 to J.R.; and BFU2008-04188-C03-03 to L.Y. (Ministry of Science and Innovation, Spain, and FEDER). Work in the laboratory of the Institute of Physiology in Prague was supported by grants MSMT LC531, GA AS CR IAA500110801, and AV0Z 50110509. J.A. was the recipient of an Ajut 2009SGR-1091 and an ICREA academia award (Generalitat de Catalunya). en_EN
dc.language Inglés es_ES
dc.publisher American Society for Microbiology es_ES
dc.relation Ministry of Science and Innovation, Spain BFU2008-04188-C03-01 es_ES
dc.relation Ministry of Science and Innovation, Spain GEN2006-27748-C2-1-E/SYS es_ES
dc.relation Ministry of Science and Innovation, Spain EUI200904147 es_ES
dc.relation Ministry of Science and Innovation, Spain GEN2006-27748-C2-2-E/SYS es_ES
dc.relation Ministry of Science and Innovation, Spain BFU2008-04188-C03-03 es_ES
dc.relation MSMT LC531 es_ES
dc.relation GA AS CR IAA500110801 es_ES
dc.relation AV0Z 50110509 es_ES
dc.relation Generalitat de Catalunya ICREA academia award es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cation transport protein es_ES
dc.subject Hygromycin B es_ES
dc.subject PMA1 protein, S cerevisiae es_ES
dc.subject Potassium es_ES
dc.subject Proton transporting adenosine triphosphatase es_ES
dc.subject Quaternary ammonium derivative es_ES
dc.subject Saccharomyces cerevisiae protein es_ES
dc.subject Spermine es_ES
dc.subject Tetramethylammonium es_ES
dc.subject TRK1 protein, S cerevisiae es_ES
dc.subject Article es_ES
dc.subject Cell membrane potential es_ES
dc.subject Genetics es_ES
dc.subject Homeostasis es_ES
dc.subject Metabolism es_ES
dc.subject Mutation es_ES
dc.subject Phenotype es_ES
dc.subject Physiology es_ES
dc.subject Saccharomyces cerevisiae es_ES
dc.subject Transport at the cellular level es_ES
dc.subject Biological Transport es_ES
dc.subject Cation Transport Proteins es_ES
dc.subject Membrane Potentials es_ES
dc.subject Proton-Translocating ATPases es_ES
dc.subject Quaternary Ammonium Compounds es_ES
dc.subject Saccharomyces cerevisiae Proteins es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1128/EC.05029-11
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Barreto, L.; Canadell, D.; Petrezselyova, S.; Navarrete, C.; Maresova, L.; Pérez Valle, J.; Herrera, R.... (2011). A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae. 10(9):1241-1250. doi:10.1128/EC.05029-11 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://ec.asm.org/content/10/9/1241.full.pdf+html es_ES
dc.description.upvformatpinicio 1241 es_ES
dc.description.upvformatpfin 1250 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.description.issue 9 es_ES
dc.relation.senia 193971


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