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Development of a chemiluminescence-based quantitatve lateral flow immunoassay for on-field detection of 2,4,6-trinitrotoluene

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Development of a chemiluminescence-based quantitatve lateral flow immunoassay for on-field detection of 2,4,6-trinitrotoluene

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dc.contributor.author Mirasoli, Mara es_ES
dc.contributor.author Buragina, Angela es_ES
dc.contributor.author Dolci, Luisa Stella es_ES
dc.contributor.author Guardigli, Massimo es_ES
dc.contributor.author Simoni, Patrizia es_ES
dc.contributor.author Montoya Baides, Ángel es_ES
dc.contributor.author Maiolini, Elisabetta es_ES
dc.contributor.author Girotti, Stefano es_ES
dc.contributor.author Roda, Aldo es_ES
dc.date.accessioned 2013-07-08T12:28:45Z
dc.date.issued 2012
dc.identifier.issn 0003-2670
dc.identifier.uri http://hdl.handle.net/10251/30826
dc.description.abstract [EN] Simple, rapid and highly sensitive assays, possibly allowing on-site analysis, are required in the security and forensic fields or to obtain early signs of environmental pollution. Several bioanalytical methods and biosensors based on portable devices have been developed for this purpose. Among them, Lateral Flow ImmunoAssays (LFIAs) offer the advantages of rapidity and ease of use and, thanks to the high specificity of antigen-antibody binding, allow greatly simplifying and reducing sample pre-analytical treatments. However, LFIAs usually employ colloidal gold or latex beads as labels and they rely on the formation of colored bands visible by the naked eye. With this assay format, only qualitative or semi-quantitative information can be obtained and low sensitivity is achieved. Recently, the use of enzyme-catalyzed chemiluminescence detection in LFIA has been proposed to overcome these problems. In this work, we describe the development of a quantitative CL-LFIA assay for the detection of 2,4,6-trinitrotoluene (TNT) in real samples. Thanks to the use of a portable imaging device for CL signal measurement based on a thermoelectrically cooled CCD camera, the analysis could be performed directly on-field. A limit of detection of 0.2¿gmL -1 TNT was obtained, which is five times lower than that obtained with a previously described colloidal gold-based LFIA developed employing the same immunoreagents. The dynamic range of the assay extended up to 5¿gmL -1 TNT and recoveries ranging from 97% to 111% were obtained in the analysis of real samples (post blast residues obtained from controlled explosion). © 2012 Elsevier B.V. es_ES
dc.description.sponsorship This work was supported by grants from the Italian Ministry of Instruction, University and Research (PRIN 2007AWK85F "Development of integrated analytical instruments based on luminescence principles for multiplexed analysis in POCT (Point-Of-Care Testing) applications" and PRIN 2006033429 "New Analytical Tools for Security and Criminal Investigations: Trace Detection and Identification of Explosives and Related Compounds") and from the University of Bologna ("Fundamental Oriented Research"). en_EN
dc.language Inglés es_ES
dc.publisher ELSEVIER SCIENCE BV es_ES
dc.relation Italian Ministry of Instruction, University and Research [2007AWK85F, 2006033429] es_ES
dc.relation University of Bologna es_ES
dc.relation.ispartof ANALYTICA CHIMICA ACTA es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject 2,4,6-Trinitrotoluene es_ES
dc.subject Biosensors es_ES
dc.subject Chemiluminescence es_ES
dc.subject Imaging es_ES
dc.subject Lateral flow immunoassay es_ES
dc.subject 2 ,4 ,6-trinitrotoluene es_ES
dc.subject Antigen-antibody binding es_ES
dc.subject Bioanalytical methods es_ES
dc.subject Chemiluminescence detection es_ES
dc.subject Colloidal gold es_ES
dc.subject Dynamic range es_ES
dc.subject Ease of use es_ES
dc.subject Environmental pollutions es_ES
dc.subject High specificity es_ES
dc.subject Highly sensitive es_ES
dc.subject Imaging device es_ES
dc.subject Immunoreagents es_ES
dc.subject Latex beads es_ES
dc.subject Limit of detection es_ES
dc.subject Low sensitivity es_ES
dc.subject Naked-eye es_ES
dc.subject On-site analysis es_ES
dc.subject Portable device es_ES
dc.subject Real samples es_ES
dc.subject Semi-quantitative es_ES
dc.subject Signal measurement es_ES
dc.subject Thermoelectrically-cooled es_ES
dc.subject Assays es_ES
dc.subject Colloids es_ES
dc.subject Imaging techniques es_ES
dc.subject Explosives detection es_ES
dc.subject Trinitrotoluene es_ES
dc.subject Accuracy es_ES
dc.subject Article es_ES
dc.subject Calibration es_ES
dc.subject Chemoluminescence es_ES
dc.subject Concentration (parameters) es_ES
dc.subject Controlled study es_ES
dc.subject Cost benefit analysis es_ES
dc.subject Field study es_ES
dc.subject Flow measurement es_ES
dc.subject Immunoassay es_ES
dc.subject Prediction es_ES
dc.subject Priority journal es_ES
dc.subject Process development es_ES
dc.subject Process optimization es_ES
dc.subject Product recovery es_ES
dc.subject Quantitative analysis es_ES
dc.subject Sensitivity and specificity es_ES
dc.subject Signal detection es_ES
dc.subject Signal noise ratio es_ES
dc.subject Antibodies es_ES
dc.subject Kinetics es_ES
dc.subject Luminescent Measurements es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title Development of a chemiluminescence-based quantitatve lateral flow immunoassay for on-field detection of 2,4,6-trinitrotoluene es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1016/j.aca.2012.01.036
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano - Institut Interuniversitari d'Investigació en Bioenginyeria i Tecnologia Orientada a l'Ésser Humà es_ES
dc.description.bibliographicCitation Mirasoli, M.; Buragina, A.; Dolci, LS.; Guardigli, M.; Simoni, P.; Montoya Baides, Á.; Maiolini, E.... (2012). Development of a chemiluminescence-based quantitatve lateral flow immunoassay for on-field detection of 2,4,6-trinitrotoluene. ANALYTICA CHIMICA ACTA. 721:167-172. doi:10.1016/j.aca.2012.01.036 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.aca.2012.01.036 es_ES
dc.description.upvformatpinicio 167 es_ES
dc.description.upvformatpfin 172 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 721 es_ES
dc.relation.senia 215070


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