- -

Subtractive imaging in Confocal Scanning Microscopy using a CCD camera as a detector

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Subtractive imaging in Confocal Scanning Microscopy using a CCD camera as a detector

Show simple item record

Files in this item

dc.contributor.author Sanchez-Ortiga, E. es_ES
dc.contributor.author Sheppard, C.J.R. es_ES
dc.contributor.author Saavedra, G. es_ES
dc.contributor.author Martinez-Corral, M. es_ES
dc.contributor.author Doblas, A. es_ES
dc.contributor.author Calatayud Calatayud, Arnau es_ES
dc.date.accessioned 2013-07-24T07:39:36Z
dc.date.available 2013-07-24T07:39:36Z
dc.date.issued 2012
dc.identifier.issn 0146-9592
dc.identifier.uri http://hdl.handle.net/10251/31362
dc.description.abstract [EN] We report a scheme for the detector system of confocal microscopes in which the pinhole and a large-area detector are substituted by a CCD camera. The numerical integration of the intensities acquired by the active pixels emulates the signal passing through the pinhole. We demonstrate the imaging capability and the optical sectioning of the system. Subtractive-imaging confocal microscopy can be implemented in a simple manner, providing superresolution and improving optical sectioning. (C) 2012 Optical Society of America es_ES
dc.description.sponsorship This work was supported by the Plan Nacional I+D+I under grant FIS2009-9135 and also by Generalitat Valenciana under grant PROMETEO2009-077.
dc.language Inglés es_ES
dc.publisher Optical Society of America es_ES
dc.relation.ispartof Optics Letters es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Microscopy es_ES
dc.subject Confocal microscopy es_ES
dc.subject Scanning microscopy es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Subtractive imaging in Confocal Scanning Microscopy using a CCD camera as a detector es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1364/OL.37.001280
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//FIS2009-09135/ES/Desarrollo De Nuevos Dispositivos De Registro Y Visualizacion De Muestras 3D Con Resolucion Submicrometrica/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO09%2F2009%2F077/ES/Grupo de fibras ópticas y procesado de señal/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Tecnologías Físicas: Acústica, Materiales y Astrofísica - Centre de Tecnologies Físiques: Acústica, Materials i Astrofísica es_ES
dc.description.bibliographicCitation Sanchez-Ortiga, E.; Sheppard, C.; Saavedra, G.; Martinez-Corral, M.; Doblas, A.; Calatayud Calatayud, A. (2012). Subtractive imaging in Confocal Scanning Microscopy using a CCD camera as a detector. Optics Letters. 37(7):1280-1282. https://doi.org/10.1364/OL.37.001280 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1364/OL.37.001280 es_ES
dc.description.upvformatpinicio 1280 es_ES
dc.description.upvformatpfin 1282 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 37 es_ES
dc.description.issue 7 es_ES
dc.relation.senia 221940
dc.identifier.pmid 22466221
dc.contributor.funder Ministerio de Ciencia e Innovación
dc.contributor.funder Generalitat Valenciana
dc.description.references Sheppard, C. J. R., & Choudhury, A. (1977). Image Formation in the Scanning Microscope. Optica Acta: International Journal of Optics, 24(10), 1051-1073. doi:10.1080/713819421 es_ES
dc.description.references Sheppard, C. J. R., & Wilson, T. (1978). Image Formation in Scanning Microscopes with Partially Coherent Source and Detector. Optica Acta: International Journal of Optics, 25(4), 315-325. doi:10.1080/713819784 es_ES
dc.description.references Sheppard, C. J. R., & Cogswell, C. J. (1990). Confocal Microscopy with Detector Arrays. Journal of Modern Optics, 37(2), 267-279. doi:10.1080/09500349014550331 es_ES
dc.description.references Pawley, J. B., Blouke, M. M., & Janesick, J. R. (1996). <title>CCDiode: an optimal detector for laser confocal microscopes</title>. Three-Dimensional Microscopy: Image Acquisition and Processing III. doi:10.1117/12.237490 es_ES
dc.description.references Müller, C. B., & Enderlein, J. (2010). Image Scanning Microscopy. Physical Review Letters, 104(19). doi:10.1103/physrevlett.104.198101 es_ES
dc.description.references Wilson, T., & Carlini, A. R. (1988). Effect of detector displacement in confocal imaging systems. Applied Optics, 27(18), 3791. doi:10.1364/ao.27.003791 es_ES
dc.description.references Heintzmann, R., Sarafis, V., Munroe, P., Nailon, J., Hanley, Q. S., & Jovin, T. M. (2003). Resolution enhancement by subtraction of confocal signals taken at different pinhole sizes. Micron, 34(6-7), 293-300. doi:10.1016/s0968-4328(03)00054-4 es_ES
dc.description.references Boyer, G., & Sarafis, V. (2001). Two pinhole superresolution using spatial filters. Optik, 112(4), 177-179. doi:10.1078/0030-4026-00033 es_ES
dc.description.references Martı́nez-Corral, M., Caballero, M. T., Ibáñez-López, C., & Sarafis, V. (2003). Optical sectioning by two-pinhole confocal fluorescence microscopy. Micron, 34(6-7), 313-318. doi:10.1016/s0968-4328(03)00057-x es_ES
dc.description.references Gu, M., & Sheppard, C. J. R. (1992). Confocal fluorescent microscopy with a finite-sized circular detector. Journal of the Optical Society of America A, 9(1), 151. doi:10.1364/josaa.9.000151 es_ES
dc.description.references Wilson1, T., & Carlini, A. R. (1987). Size of the detector in confocal imaging systems. Optics Letters, 12(4), 227. doi:10.1364/ol.12.000227 es_ES


This item appears in the following Collection(s)

Show simple item record