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Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures

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Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures

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dc.contributor.author Salas Aragon, Pablo es_ES
dc.contributor.author Rivas Sendra, Alba es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.contributor.author Seguí-Simarro, José M.
dc.date.accessioned 2013-10-31T13:10:25Z
dc.date.issued 2012
dc.identifier.issn 0014-2336
dc.identifier.uri http://hdl.handle.net/10251/33167
dc.description.abstract In this work we address two aspects of eggplant flower biology potentially involved on the efficiency of anther culture: the selection of the best floral stage to extract anthers for culture, and the effect of heterostyly in the identification of suitable buds and anthers. For 12 different accessions, we determined morphological criteria (length ranges) to identify buds and anthers enriched in vacuolate microspores and young bicellular pollen, the stages most responsive to embryogenesis induction. While these microspore/pollen stages were the most responsive when isolated and cultured in liquid medium, we observed that culture of anthers containing these stages is not the best choice. Instead, the highest response was found for younger anthers, containing mostly young and mid microspores. We analyzed eggplant anther walls and found that their particular thickness may be behind this apparent discrepancy, since they may delay the diffusion of inducing factors to the anther locule, reducing their effect over inducible microspores. Thus, the culture of younger anthers would allow for younger microspores to grow up to the inducible stages while factors are entering the locule. We also analyzed the embryogenic response of short and long-styled buds present in Cristal, a heterostylic cultivar. Our results demonstrated that each floral morph produced buds and anthers of different lengths, but equally useful for anther culture, since similar amounts of embryos were produced. The practical application of these results may improve the efficiency of anther culture not only in these cultivars, but in others also presenting thick anther walls and heterostyly. © 2011 Springer Science+Business Media B.V. es_ES
dc.description.sponsorship We want to acknowledge Drs. Begona Renau and Antonio Serrano, and Mrs Nuria Palacios for their excellent technical work, as well as the staff of the COMAV greenhouses for their valuable help. We also thank the anonymous reviewers for their excellent criticisms and the ideas they contributed. This work was supported by grant AGL2010-17895 from Spanish MICINN to JMSS. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation Spanish MICINN [AGL2010-17895] es_ES
dc.relation.ispartof Euphytica es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Androgenesis es_ES
dc.subject Anther culture es_ES
dc.subject Doubled haploid es_ES
dc.subject Haploid es_ES
dc.subject Microspore embryogenesis es_ES
dc.subject Solanum aethiopicum es_ES
dc.subject Solanum incanum es_ES
dc.subject Solanum macrocarpon es_ES
dc.subject Solanum melongena es_ES
dc.subject Solanum es_ES
dc.subject.classification GENETICA es_ES
dc.title Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1007/s10681-011-0569-9
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Salas Aragon, P.; Rivas Sendra, A.; Prohens Tomás, J.; Seguí-Simarro, JM. (2012). Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures. Euphytica. 184(2):235-250. doi:10.1007/s10681-011-0569-9 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/ 10.1007/s10681-011-0569-9 es_ES
dc.description.upvformatpinicio 235 es_ES
dc.description.upvformatpfin 250 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 184 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 209312
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