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A novel role for protein kinase Gcn2 in yeast tolerance to intracellular acid stress

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A novel role for protein kinase Gcn2 in yeast tolerance to intracellular acid stress

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dc.contributor.author Hueso Lorente, Guillem es_ES
dc.contributor.author Aparicio Sanchis, Rafael es_ES
dc.contributor.author Montesinos De Lago, Consuelo es_ES
dc.contributor.author Lorenz, Silvia es_ES
dc.contributor.author Murguía Ibáñez, José Ramón es_ES
dc.contributor.author Serrano Salom, Ramón es_ES
dc.date.accessioned 2013-11-19T15:15:39Z
dc.date.issued 2012
dc.identifier.issn 0264-6021
dc.identifier.uri http://hdl.handle.net/10251/33762
dc.description.abstract Intracellular pH conditions many cellular systems, but its mechanisms of regulation and perception are mostly unknown. We have identified two yeast genes important for tolerance to intracellular acidification caused by weak permeable acids. One corresponded to LEU2 and functions by removing the dependency of the leu2 mutant host strain on uptake of extracellular leucine. Leucine transport is inhibited by intracellular acidification, and either leucine oversupplementation or overexpression of the transporter gene BAP2 improved acid growth. Another acid-tolerance gene is GCN2, encoding a protein kinase activated by uncharged tRNAs during amino acid starvation. Gcn2 phosphorylates eIF2¿ (eukaryotic initiation factor 2¿) (Sui2) at Ser51 and this inhibits general translation, but activates that of Gcn4, a transcription factor for amino acid biosynthetic genes. Intracellular acidification activates Gcn2 probably by inhibition of aminoacyl-tRNA synthetases because we observed accumulation of uncharged tRNAleu without leucine depletion. Gcn2 is required for leucine transport and a gcn2-null mutant is sensitive to acid stress if auxotrophic for leucine. Gcn4 is required for neither leucine transport nor acid tolerance, but a S51A sui2 mutant is acid-sensitive. This suggests that Gcn2, by phosphorylating eIF2¿, may activate translation of an unknown regulator of amino acid transporters different from Gcn4. es_ES
dc.description.sponsorship This work was funded by grants from the Spanish Ministerio de Ciencia e Innovacion (Madrid) [grant number BFU2008-00604] and the Generalitat Valenciana (Valencia) [grant number Prometeo/2010/038]. en_EN
dc.format.extent 10 es_ES
dc.language Inglés es_ES
dc.publisher Portland Press es_ES
dc.relation.ispartof Biochemical Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Ph homeostasis es_ES
dc.subject Signal transduction es_ES
dc.subject Gcn2 es_ES
dc.subject Saccharomyces cerevisiae es_ES
dc.subject Amino acid transport es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title A novel role for protein kinase Gcn2 in yeast tolerance to intracellular acid stress es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1042/BJ20111264
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2008-00604/ES/REGULACION DE H+-ATPASA, TRANSPORTE DE K+ Y TOR: INVESTIGANDO LA RELACION ENTRE DETERMINANTES BIOFISICOS Y BIOQUIMICOS DEL CRECIMIENTO CELULAR EN ARABIDOPSIS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F038/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Hueso Lorente, G.; Aparicio Sanchis, R.; Montesinos De Lago, C.; Lorenz, S.; Murguía Ibáñez, JR.; Serrano Salom, R. (2012). A novel role for protein kinase Gcn2 in yeast tolerance to intracellular acid stress. Biochemical Journal. 441(1):255-264. https://doi.org/10.1042/BJ20111264 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi:10.1042/BJ20111264 es_ES
dc.description.upvformatpinicio 255 es_ES
dc.description.upvformatpfin 264 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 441 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 232564
dc.identifier.eissn 1470-8728
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Generalitat Valenciana es_ES
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