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Involvement of the Chloroplastic Isoform of tRNA Ligase in the Replication of Viroids Belonging to the Family Avsunviroidae

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Involvement of the Chloroplastic Isoform of tRNA Ligase in the Replication of Viroids Belonging to the Family Avsunviroidae

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dc.contributor.author Nohales Zafra, Maria Angeles es_ES
dc.contributor.author Molina Serrano, Diego es_ES
dc.contributor.author Flores Pedauye, Ricardo es_ES
dc.contributor.author Daros Arnau, Jose Antonio es_ES
dc.date.accessioned 2013-12-16T12:51:54Z
dc.date.available 2013-12-16T12:51:54Z
dc.date.issued 2012
dc.identifier.issn 0022-538X
dc.identifier.uri http://hdl.handle.net/10251/34533
dc.description.abstract Avocado sunblotch viroid, peach latent mosaic viroid, chrysanthemum chlorotic mottle viroid, and eggplant latent viroid (ELVd), the four recognized members of the family Avsunviroidae, replicate through the symmetric pathway of an RNA-to-RNA rolling-circle mechanism in chloroplasts of infected cells. Viroid oligomeric transcripts of both polarities contain embedded hammerhead ribozymes that, during replication, mediate their self-cleavage to monomeric-length RNAs with 5'-hydroxyl and 2',3'-phosphodiester termini that are subsequently circularized. We report that a recombinant version of the chloroplastic isoform of the tRNA ligase from eggplant (Solanum melongena L.) efficiently catalyzes in vitro circularization of the plus [(+)] and minus [(-)] monomeric linear replication intermediates from the four Avsunviroidae. We also show that while this RNA ligase specifically recognizes the genuine monomeric linear (+) ELVd replication intermediate, it does not do so with five other monomeric linear (+) ELVd RNAs with their ends mapping at different sites along the molecule, despite containing the same 5'-hydroxyl and 2',3'-phosphodiester terminal groups. Moreover, experiments involving transient expression of a dimeric (+) ELVd transcript in Nicotiana benthamiana Domin plants preinoculated with a tobacco rattle virus-derived vector to induce silencing of the plant endogenous tRNA ligase show a significant reduction of ELVd circularization. In contrast, circularization of a viroid replicating in the nucleus occurring through a different pathway is unaffected. Together, these results support the conclusion that the chloroplastic isoform of the plant tRNA ligase is the host enzyme mediating circularization of both (+) and (-) monomeric linear intermediates during replication of the viroids belonging to the family Avsunviroidae. es_ES
dc.description.sponsorship This work was supported by the Ministerio de Ciencia e Innovacion (MICINN) from Spain through grants BIO2008-01986, BIO2011-26741, and BFU2008-03154. M. A. Nohales and D. Molina-Serrano were the recipients of predoctoral fellowships from the Spanish Ministerio de Educacion y Ciencia. en_EN
dc.language Inglés es_ES
dc.publisher American Society for Microbiology es_ES
dc.relation.ispartof Journal of Virology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Hammerhead ribozyme es_ES
dc.subject Self-cleavage es_ES
dc.subject In-vivo es_ES
dc.subject Wheat-germ es_ES
dc.subject Host interactions es_ES
dc.subject Ligation es_ES
dc.subject Virus es_ES
dc.subject Vitro es_ES
dc.subject Plus es_ES
dc.subject Transcripts es_ES
dc.title Involvement of the Chloroplastic Isoform of tRNA Ligase in the Replication of Viroids Belonging to the Family Avsunviroidae es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1128/JVI.00629-12
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2008-01986/ES/INTERACCIONES RNA-PROTEINA EN EL CICLO INFECCIOSO DE PATOGENOS DE RNA DE PLANTAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2011-26741/ES/PATOGENOS DE RNA DE PLANTAS: INTERACCION CON EL HUESPED Y DESARROLLO DE HERRAMIENTAS BIOTECNOLOGICAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2008-03154/ES/INTERACCIONES VIROIDE-HUESPED: PAPEL DE LAS RIBOZIMAS, DEL SILENCIAMIENTO MEDIADO POR RNA, Y DE LA RECOMBINACION DE RNA/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Nohales Zafra, MA.; Molina Serrano, D.; Flores Pedauye, R.; Daros Arnau, JA. (2012). Involvement of the Chloroplastic Isoform of tRNA Ligase in the Replication of Viroids Belonging to the Family Avsunviroidae. Journal of Virology. 86:8269-8276. https://doi.org/10.1128/JVI.00629-12 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1128/JVI.00629-12 es_ES
dc.description.upvformatpinicio 8269 es_ES
dc.description.upvformatpfin 8276 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 86 es_ES
dc.relation.senia 233016
dc.identifier.pmid 22623792 en_EN
dc.identifier.pmcid PMC3421689 en_EN
dc.contributor.funder Ministerio de Educación y Ciencia es_ES
dc.description.references Abelson, J., Trotta, C. R., & Li, H. (1998). tRNA Splicing. Journal of Biological Chemistry, 273(21), 12685-12688. doi:10.1074/jbc.273.21.12685 es_ES
dc.description.references Branch, A. D., Benenfeld, B. J., & Robertson, H. D. (1988). Evidence for a single rolling circle in the replication of potato spindle tuber viroid. Proceedings of the National Academy of Sciences, 85(23), 9128-9132. doi:10.1073/pnas.85.23.9128 es_ES
dc.description.references Branch, A., & Robertson, H. (1984). A replication cycle for viroids and other small infectious RNA’s. Science, 223(4635), 450-455. doi:10.1126/science.6197756 es_ES
dc.description.references Canny, M. D., Jucker, F. M., & Pardi, A. (2007). Efficient Ligation of theSchistosomaHammerhead Ribozyme†. Biochemistry, 46(12), 3826-3834. doi:10.1021/bi062077r es_ES
dc.description.references Cote, F., Levesque, D., & Perreault, J.-P. (2001). Natural 2’,5’-Phosphodiester Bonds Found at the Ligation Sites of Peach Latent Mosaic Viroid. Journal of Virology, 75(1), 19-25. doi:10.1128/jvi.75.1.19-25.2001 es_ES
dc.description.references Côté, F., & Perreault, J.-P. (1997). Peach latent mosaic viroid is locked by a 2′,5′-phosphodiester bond produced by in vitro self-ligation 1 1Edited by D. E. Draper. Journal of Molecular Biology, 273(3), 533-543. doi:10.1006/jmbi.1997.1355 es_ES
dc.description.references Daros, J.-A. (2002). A chloroplast protein binds a viroid RNA in vivo and facilitates its hammerhead-mediated self-cleavage. The EMBO Journal, 21(4), 749-759. doi:10.1093/emboj/21.4.749 es_ES
dc.description.references Daros, J. A., Marcos, J. F., Hernandez, C., & Flores, R. (1994). Replication of avocado sunblotch viroid: evidence for a symmetric pathway with two rolling circles and hammerhead ribozyme processing. Proceedings of the National Academy of Sciences, 91(26), 12813-12817. doi:10.1073/pnas.91.26.12813 es_ES
dc.description.references De la Pena, M. (2003). Peripheral regions of natural hammerhead ribozymes greatly increase their self-cleavage activity. The EMBO Journal, 22(20), 5561-5570. doi:10.1093/emboj/cdg530 es_ES
dc.description.references De la Pena, M., Navarro, B., & Flores, R. (1999). Mapping the molecular determinant of pathogenicity in a hammerhead viroid: A tetraloop within the in vivo branched RNA conformation. Proceedings of the National Academy of Sciences, 96(17), 9960-9965. doi:10.1073/pnas.96.17.9960 es_ES
dc.description.references Ding, B. (2009). The Biology of Viroid-Host Interactions. Annual Review of Phytopathology, 47(1), 105-131. doi:10.1146/annurev-phyto-080508-081927 es_ES
dc.description.references Englert, M. (2005). Plant tRNA ligases are multifunctional enzymes that have diverged in sequence and substrate specificity from RNA ligases of other phylogenetic origins. Nucleic Acids Research, 33(1), 388-399. doi:10.1093/nar/gki174 es_ES
dc.description.references Englert, M., Latz, A., Becker, D., Gimple, O., Beier, H., & Akama, K. (2007). Plant pre-tRNA splicing enzymes are targeted to multiple cellular compartments. Biochimie, 89(11), 1351-1365. doi:10.1016/j.biochi.2007.06.014 es_ES
dc.description.references Fadda, Z., Daros, J. A., Fagoaga, C., Flores, R., & Duran-Vila, N. (2003). Eggplant Latent Viroid, the Candidate Type Species for a New Genus within the Family Avsunviroidae (Hammerhead Viroids). Journal of Virology, 77(11), 6528-6532. doi:10.1128/jvi.77.11.6528-6532.2003 es_ES
dc.description.references Feldstein, P. A., Hu, Y., & Owens, R. A. (1998). Precisely full length, circularizable, complementary RNA: An infectious form of potato spindle tuber viroid. Proceedings of the National Academy of Sciences, 95(11), 6560-6565. doi:10.1073/pnas.95.11.6560 es_ES
dc.description.references Flores, R., Daròs, J.-A., & Hernández, C. (2000). Avsunviroidae family: Viroids containing hammerhead ribozymes. Advances in Virus Research, 271-323. doi:10.1016/s0065-3527(00)55006-4 es_ES
dc.description.references Flores, R., Hernández, C., Alba, A. E. M. de, Daròs, J.-A., & Serio, F. D. (2005). Viroids and Viroid-Host Interactions. Annual Review of Phytopathology, 43(1), 117-139. doi:10.1146/annurev.phyto.43.040204.140243 es_ES
dc.description.references Flores, R., & Owens, R. A. (2008). Viroids. Encyclopedia of Virology, 332-342. doi:10.1016/b978-012374410-4.00532-x es_ES
dc.description.references Gas, M.-E., Hernández, C., Flores, R., & Daròs, J.-A. (2007). Processing of Nuclear Viroids In Vivo: An Interplay between RNA Conformations. PLoS Pathogens, 3(11), e182. doi:10.1371/journal.ppat.0030182 es_ES
dc.description.references Gas, M.-E., Molina-Serrano, D., Hernandez, C., Flores, R., & Daros, J.-A. (2008). Monomeric Linear RNA of Citrus Exocortis Viroid Resulting from Processing In Vivo Has 5’-Phosphomonoester and 3’-Hydroxyl Termini: Implications for the RNase and RNA Ligase Involved in Replication. Journal of Virology, 82(20), 10321-10325. doi:10.1128/jvi.01229-08 es_ES
dc.description.references Gómez, G., & Pallás, V. (2010). Noncoding RNA Mediated Traffic of Foreign mRNA into Chloroplasts Reveals a Novel Signaling Mechanism in Plants. PLoS ONE, 5(8), e12269. doi:10.1371/journal.pone.0012269 es_ES
dc.description.references Hernandez, C., & Flores, R. (1992). Plus and minus RNAs of peach latent mosaic viroid self-cleave in vitro via hammerhead structures. Proceedings of the National Academy of Sciences, 89(9), 3711-3715. doi:10.1073/pnas.89.9.3711 es_ES
dc.description.references Hertel, K. J., Herschlag, D., & Uhlenbeck, O. C. (1994). A Kinetic and Thermodynamic Framework for the Hammerhead Ribozyme Reaction. Biochemistry, 33(11), 3374-3385. doi:10.1021/bi00177a031 es_ES
dc.description.references Hutchins, C. J., Keese, P., Visvader, J. E., Rathjen, P. D., McInnes, J. L., & Symons, R. H. (1985). Comparison of multimeric plus and minus forms of viroids and virusoids. Plant Molecular Biology, 4(5), 293-304. doi:10.1007/bf02418248 es_ES
dc.description.references Hutchins, C. J., Rathjen, P. D., Forster, A. C., & Symons, R. H. (1986). Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroid. Nucleic Acids Research, 14(9), 3627-3640. doi:10.1093/nar/14.9.3627 es_ES
dc.description.references Khvorova, A., Lescoute, A., Westhof, E., & Jayasena, S. D. (2003). Sequence elements outside the hammerhead ribozyme catalytic core enable intracellular activity. Nature Structural & Molecular Biology, 10(9), 708-712. doi:10.1038/nsb959 es_ES
dc.description.references Kiberstis, P. A., Haseloff, J., & Zimmern, D. (1985). 2′ phosphomonoester, 3′-5′ phosphodiester bond at a unique site in a circular viral RNA. The EMBO Journal, 4(3), 817-822. doi:10.1002/j.1460-2075.1985.tb03703.x es_ES
dc.description.references Konarska, M., Filipowicz, W., Domdey, H., & Gross, H. J. (1981). Formation of a 2′-phosphomonoester, 3′,5′-phosphodiester linkage by a novel RNA ligase in wheat germ. Nature, 293(5828), 112-116. doi:10.1038/293112a0 es_ES
dc.description.references Liu, Y., Schiff, M., Marathe, R., & Dinesh-Kumar, S. P. (2002). Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus. The Plant Journal, 30(4), 415-429. doi:10.1046/j.1365-313x.2002.01297.x es_ES
dc.description.references Makino, S., Sawasaki, T., Endo, Y., & Takai, K. (2005). Purification and sequence determination of an RNA ligase from wheat embryos. Nucleic Acids Symposium Series, 49(1), 319-320. doi:10.1093/nass/49.1.319 es_ES
dc.description.references Marcos, J. F., & Flores, R. (1993). The 5’ end Generated in the in vitro Self-Cleavage Reaction of Avocado Sunblotch Viroid RNAs is Present in Naturally Occurring Linear Viroid Molecules. Journal of General Virology, 74(5), 907-910. doi:10.1099/0022-1317-74-5-907 es_ES
dc.description.references Martinez, F., Marques, J., Salvador, M. L., & Daros, J.-A. (2009). Mutational analysis of eggplant latent viroid RNA processing in Chlamydomonas reinhardtii chloroplast. Journal of General Virology, 90(12), 3057-3065. doi:10.1099/vir.0.013425-0 es_ES
dc.description.references Molina-Serrano, D., Marqués, J., Nohales, M.-Á., Flores, R., & Daròs, J.-A. (2012). A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2´–5′ RNA ligase. RNA Biology, 9(3), 326-333. doi:10.4161/rna.19218 es_ES
dc.description.references Navarro, B., & Flores, R. (1997). Chrysanthemum chlorotic mottle viroid: Unusual structural properties of a subgroup of self-cleaving viroids with hammerhead ribozymes. Proceedings of the National Academy of Sciences, 94(21), 11262-11267. doi:10.1073/pnas.94.21.11262 es_ES
dc.description.references Navarro, J.-A., Daròs, J.-A., & Flores, R. (1999). Complexes Containing Both Polarity Strands of Avocado Sunblotch Viroid: Identification in Chloroplasts and Characterization. Virology, 253(1), 77-85. doi:10.1006/viro.1998.9497 es_ES
dc.description.references Navarro, J.-A., Vera, A., & Flores, R. (2000). A Chloroplastic RNA Polymerase Resistant to Tagetitoxin Is Involved in Replication of Avocado Sunblotch Viroid. Virology, 268(1), 218-225. doi:10.1006/viro.1999.0161 es_ES
dc.description.references Nelson, J. A., Shepotinovskaya, I., & Uhlenbeck, O. C. (2005). Hammerheads Derived from sTRSV Show Enhanced Cleavage and Ligation Rate Constants†. Biochemistry, 44(44), 14577-14585. doi:10.1021/bi051130t es_ES
dc.description.references PRODY, G. A., BAKOS, J. T., BUZAYAN, J. M., SCHNEIDER, I. R., & BRUENING, G. (1986). Autolytic Processing of Dimeric Plant Virus Satellite RNA. Science, 231(4745), 1577-1580. doi:10.1126/science.231.4745.1577 es_ES
dc.description.references Rodio, M.-E., Delgado, S., De Stradis, A., Gómez, M.-D., Flores, R., & Di Serio, F. (2007). A Viroid RNA with a Specific Structural Motif Inhibits Chloroplast Development. The Plant Cell, 19(11), 3610-3626. doi:10.1105/tpc.106.049775 es_ES
dc.description.references Ruiz, M. T., Voinnet, O., & Baulcombe, D. C. (1998). Initiation and Maintenance of Virus-Induced Gene Silencing. The Plant Cell, 10(6), 937-946. doi:10.1105/tpc.10.6.937 es_ES
dc.description.references Schurer, H. (2002). A universal method to produce in vitro transcripts with homogeneous 3’ ends. Nucleic Acids Research, 30(12), 56e-56. doi:10.1093/nar/gnf055 es_ES
dc.description.references Tsagris, E. M., Martínez de Alba, Á. E., Gozmanova, M., & Kalantidis, K. (2008). Viroids. Cellular Microbiology, 10(11), 2168-2179. doi:10.1111/j.1462-5822.2008.01231.x es_ES
dc.description.references WANG, L. K. (2005). Structure-function analysis of yeast tRNA ligase. RNA, 11(6), 966-975. doi:10.1261/rna.2170305 es_ES


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