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Involvement of the Chloroplastic Isoform of tRNA Ligase in the Replication of Viroids Belonging to the Family Avsunviroidae

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Involvement of the Chloroplastic Isoform of tRNA Ligase in the Replication of Viroids Belonging to the Family Avsunviroidae

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dc.contributor.author Nohales Zafra, Maria Angeles es_ES
dc.contributor.author Molina Serrano, Diego es_ES
dc.contributor.author Flores Pedauye, Ricardo es_ES
dc.contributor.author Daros Arnau, Jose Antonio es_ES
dc.date.accessioned 2013-12-16T12:51:54Z
dc.date.available 2013-12-16T12:51:54Z
dc.date.issued 2012
dc.identifier.issn 0022-538X
dc.identifier.uri http://hdl.handle.net/10251/34533
dc.description.abstract Avocado sunblotch viroid, peach latent mosaic viroid, chrysanthemum chlorotic mottle viroid, and eggplant latent viroid (ELVd), the four recognized members of the family Avsunviroidae, replicate through the symmetric pathway of an RNA-to-RNA rolling-circle mechanism in chloroplasts of infected cells. Viroid oligomeric transcripts of both polarities contain embedded hammerhead ribozymes that, during replication, mediate their self-cleavage to monomeric-length RNAs with 5'-hydroxyl and 2',3'-phosphodiester termini that are subsequently circularized. We report that a recombinant version of the chloroplastic isoform of the tRNA ligase from eggplant (Solanum melongena L.) efficiently catalyzes in vitro circularization of the plus [(+)] and minus [(-)] monomeric linear replication intermediates from the four Avsunviroidae. We also show that while this RNA ligase specifically recognizes the genuine monomeric linear (+) ELVd replication intermediate, it does not do so with five other monomeric linear (+) ELVd RNAs with their ends mapping at different sites along the molecule, despite containing the same 5'-hydroxyl and 2',3'-phosphodiester terminal groups. Moreover, experiments involving transient expression of a dimeric (+) ELVd transcript in Nicotiana benthamiana Domin plants preinoculated with a tobacco rattle virus-derived vector to induce silencing of the plant endogenous tRNA ligase show a significant reduction of ELVd circularization. In contrast, circularization of a viroid replicating in the nucleus occurring through a different pathway is unaffected. Together, these results support the conclusion that the chloroplastic isoform of the plant tRNA ligase is the host enzyme mediating circularization of both (+) and (-) monomeric linear intermediates during replication of the viroids belonging to the family Avsunviroidae. es_ES
dc.description.sponsorship This work was supported by the Ministerio de Ciencia e Innovacion (MICINN) from Spain through grants BIO2008-01986, BIO2011-26741, and BFU2008-03154. M. A. Nohales and D. Molina-Serrano were the recipients of predoctoral fellowships from the Spanish Ministerio de Educacion y Ciencia. en_EN
dc.language Inglés es_ES
dc.publisher American Society for Microbiology es_ES
dc.relation Ministerio de Ciencia e Innovacion (MICINN) from Spain BIO2008-01986 es_ES
dc.relation Ministerio de Ciencia e Innovacion (MICINN) from Spain BIO2011-26741 es_ES
dc.relation Ministerio de Ciencia e Innovacion (MICINN) from Spain BFU2008-03154 es_ES
dc.relation Spanish Ministerio de Educacion y Ciencia es_ES
dc.relation.ispartof Journal of Virology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Hammerhead ribozyme es_ES
dc.subject Self-cleavage es_ES
dc.subject In-vivo es_ES
dc.subject Wheat-germ es_ES
dc.subject Host interactions es_ES
dc.subject Ligation es_ES
dc.subject Virus es_ES
dc.subject Vitro es_ES
dc.subject Plus es_ES
dc.subject Transcripts es_ES
dc.title Involvement of the Chloroplastic Isoform of tRNA Ligase in the Replication of Viroids Belonging to the Family Avsunviroidae es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1128/JVI.00629-12
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Nohales Zafra, MA.; Molina Serrano, D.; Flores Pedauye, R.; Daros Arnau, JA. (2012). Involvement of the Chloroplastic Isoform of tRNA Ligase in the Replication of Viroids Belonging to the Family Avsunviroidae. Journal of Virology. 86:8269-8276. doi:10.1128/JVI.00629-12 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1128/JVI.00629-12 es_ES
dc.description.upvformatpinicio 8269 es_ES
dc.description.upvformatpfin 8276 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 86 es_ES
dc.relation.senia 233016
dc.identifier.pmid 22623792 en_EN
dc.identifier.pmcid PMC3421689 en_EN


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