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Amidase-responsive controlled release of antitumoral drug into intracellular media using gluconamide-capped mesoporous silica nanoparticles

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Amidase-responsive controlled release of antitumoral drug into intracellular media using gluconamide-capped mesoporous silica nanoparticles

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dc.contributor.author Candel Busquets, Inmaculada es_ES
dc.contributor.author Aznar Gimeno, Elena es_ES
dc.contributor.author Mondragón Martínez, Laura es_ES
dc.contributor.author De la Torre Paredes, Cristina es_ES
dc.contributor.author Martínez Mañez, Ramón es_ES
dc.contributor.author Sancenón Galarza, Félix es_ES
dc.contributor.author Marcos Martínez, María Dolores es_ES
dc.contributor.author Amoros del Toro, Pedro Jose es_ES
dc.contributor.author Guillem, Carmen es_ES
dc.contributor.author Pérez Payá, Enrique es_ES
dc.contributor.author Costero Nieto, Ana María es_ES
dc.contributor.author Gil Grau, Salvador
dc.contributor.author Parra Álvarez, Margarita
dc.date.accessioned 2014-05-09T12:24:33Z
dc.date.issued 2012-10-02
dc.identifier.issn 2040-3364
dc.identifier.uri http://hdl.handle.net/10251/37356
dc.description.abstract MCM-41 silica nanoparticles were used as inorganic scaffolding to prepare a nanoscopic-capped hybrid material S1, which was able to release an entrapped cargo in the presence of certain enzymes, whereas in the absence of enzymes, a zero release system was obtained. S1 was prepared by loading nanoparticles with Safranine O dye and was then capped with a gluconamide derivative. In the absence of enzymes, the release of the dye from the aqueous suspensions of S1 was inhibited as a result of the steric hindrance imposed by the bulky gluconamide derivative, the polymerized gluconamide layer and the formation of a dense hydrogen-bonded network around the pore outlets. Upon the addition of amidase and pronase enzymes, delivery of Safranine O dye was observed due to the enzymatic hydrolysis of the amide bond in the anchored gluconamide derivative. S1 nanoparticles were not toxic for cells, as demonstrated by cell viability assays using HeLa and MCF-7 cell lines, and were associated with lysosomes, as shown by confocal microscopy. Finally, the S1¿CPT material loaded with the cytotoxic drug camptothecin and capped with the gluconamide derivative was prepared. The HeLa cells treated with S1¿CPT underwent cell death as a result of material internalization, and of the subsequent cellular enzyme-mediated hydrolysis and aperture of the molecular gate, which induced the release of the camptothecin cargo. es_ES
dc.description.sponsorship We thank the Spanish Government (Project MAT2009-14564-C04 and SAF2010-15512) and the Generalitat Valenciana (Project PROMETEO/2009/016and/2010/005) for support. I. C. thanks the Universitat Politecnica de Valencia for her fellowship. L. M. thanks the Generalitat Valenciana for her post-doctoral VALi+d contract. E. A. and C. T. also thank the CIBER-BBN for contracts. We thank Eva Maria Lafuente Villarreal and Alberto Hernandez Cano from the Confocal Microscopy service of CIPF and the Electronic Microscopy service of UPV for their technical support. en_EN
dc.language Inglés es_ES
dc.publisher Royal Society of Chemistry es_ES
dc.relation Spanish Government (Project MAT2009-14564-C04)
dc.relation Spanish Government (SAF2010-15512)
dc.relation Generalitat Valenciana (Project PROMETEO/2009/016)
dc.relation Generalitat Valenciana (Project PROMETEO/2010/005)
dc.relation CIBER-BBN
dc.relation.ispartof Nanoscale es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Amidohydrolases es_ES
dc.subject Antineoplastic Agents es_ES
dc.subject Camptothecin es_ES
dc.subject Cell Survival es_ES
dc.subject Drug effects es_ES
dc.subject Gluconates es_ES
dc.subject HeLa Cells es_ES
dc.subject Hydrogen Bonding es_ES
dc.subject Lysosomes es_ES
dc.subject MCF-7 Cells es_ES
dc.subject Nanoparticles es_ES
dc.subject Porosity es_ES
dc.subject Silicon Dioxide es_ES
dc.subject Electron Microscopy Service of the UPV es_ES
dc.subject.classification INGENIERIA DE LA CONSTRUCCION es_ES
dc.subject.classification QUIMICA INORGANICA es_ES
dc.subject.classification QUIMICA ORGANICA es_ES
dc.title Amidase-responsive controlled release of antitumoral drug into intracellular media using gluconamide-capped mesoporous silica nanoparticles es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1039/c2nr32062b
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Candel Busquets, I.; Aznar Gimeno, E.; Mondragón Martínez, L.; De La Torre Paredes, C.; Martínez Mañez, R.; Sancenón Galarza, F.; Marcos Martínez, MD.... (2012). Amidase-responsive controlled release of antitumoral drug into intracellular media using gluconamide-capped mesoporous silica nanoparticles. Nanoscale. 4(22):7237-7245. doi:10.1039/c2nr32062b es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1039/c2nr32062b es_ES
dc.description.upvformatpinicio 7237 es_ES
dc.description.upvformatpfin 7245 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 4 es_ES
dc.description.issue 22 es_ES
dc.relation.senia 230314


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