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dc.contributor.author | Candel Busquets, Inmaculada | es_ES |
dc.contributor.author | Aznar Gimeno, Elena | es_ES |
dc.contributor.author | Mondragón Martínez, Laura | es_ES |
dc.contributor.author | De la Torre Paredes, Cristina | es_ES |
dc.contributor.author | Martínez Mañez, Ramón | es_ES |
dc.contributor.author | Sancenón Galarza, Félix | es_ES |
dc.contributor.author | Marcos Martínez, María Dolores | es_ES |
dc.contributor.author | Amoros del Toro, Pedro Jose | es_ES |
dc.contributor.author | Guillem, Carmen | es_ES |
dc.contributor.author | Pérez Payá, Enrique | es_ES |
dc.contributor.author | Costero Nieto, Ana María | es_ES |
dc.contributor.author | Gil Grau, Salvador | |
dc.contributor.author | Parra Álvarez, Margarita | |
dc.date.accessioned | 2014-05-09T12:24:33Z | |
dc.date.issued | 2012-10-02 | |
dc.identifier.issn | 2040-3364 | |
dc.identifier.uri | http://hdl.handle.net/10251/37356 | |
dc.description.abstract | MCM-41 silica nanoparticles were used as inorganic scaffolding to prepare a nanoscopic-capped hybrid material S1, which was able to release an entrapped cargo in the presence of certain enzymes, whereas in the absence of enzymes, a zero release system was obtained. S1 was prepared by loading nanoparticles with Safranine O dye and was then capped with a gluconamide derivative. In the absence of enzymes, the release of the dye from the aqueous suspensions of S1 was inhibited as a result of the steric hindrance imposed by the bulky gluconamide derivative, the polymerized gluconamide layer and the formation of a dense hydrogen-bonded network around the pore outlets. Upon the addition of amidase and pronase enzymes, delivery of Safranine O dye was observed due to the enzymatic hydrolysis of the amide bond in the anchored gluconamide derivative. S1 nanoparticles were not toxic for cells, as demonstrated by cell viability assays using HeLa and MCF-7 cell lines, and were associated with lysosomes, as shown by confocal microscopy. Finally, the S1¿CPT material loaded with the cytotoxic drug camptothecin and capped with the gluconamide derivative was prepared. The HeLa cells treated with S1¿CPT underwent cell death as a result of material internalization, and of the subsequent cellular enzyme-mediated hydrolysis and aperture of the molecular gate, which induced the release of the camptothecin cargo. | es_ES |
dc.description.sponsorship | We thank the Spanish Government (Project MAT2009-14564-C04 and SAF2010-15512) and the Generalitat Valenciana (Project PROMETEO/2009/016and/2010/005) for support. I. C. thanks the Universitat Politecnica de Valencia for her fellowship. L. M. thanks the Generalitat Valenciana for her post-doctoral VALi+d contract. E. A. and C. T. also thank the CIBER-BBN for contracts. We thank Eva Maria Lafuente Villarreal and Alberto Hernandez Cano from the Confocal Microscopy service of CIPF and the Electronic Microscopy service of UPV for their technical support. | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Royal Society of Chemistry | es_ES |
dc.relation.ispartof | Nanoscale | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Amidohydrolases | es_ES |
dc.subject | Antineoplastic Agents | es_ES |
dc.subject | Camptothecin | es_ES |
dc.subject | Cell Survival | es_ES |
dc.subject | Drug effects | es_ES |
dc.subject | Gluconates | es_ES |
dc.subject | HeLa Cells | es_ES |
dc.subject | Hydrogen Bonding | es_ES |
dc.subject | Lysosomes | es_ES |
dc.subject | MCF-7 Cells | es_ES |
dc.subject | Nanoparticles | es_ES |
dc.subject | Porosity | es_ES |
dc.subject | Silicon Dioxide | es_ES |
dc.subject | Electron Microscopy Service of the UPV | es_ES |
dc.subject.classification | INGENIERIA DE LA CONSTRUCCION | es_ES |
dc.subject.classification | QUIMICA INORGANICA | es_ES |
dc.subject.classification | QUIMICA ORGANICA | es_ES |
dc.title | Amidase-responsive controlled release of antitumoral drug into intracellular media using gluconamide-capped mesoporous silica nanoparticles | es_ES |
dc.type | Artículo | es_ES |
dc.embargo.lift | 10000-01-01 | |
dc.embargo.terms | forever | es_ES |
dc.identifier.doi | 10.1039/c2nr32062b | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//MAT2009-14564-C04-04/ES/Nanomateriales Mesoporosos Y Particulados Para El Diseño De Sistemas Sensores, Remediacion Y Aplicaciones Relacionadas Con La Energia/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//MAT2009-14564-C04-01/ES/Nanomateriales Hibridos Para El Desarrollo De \"Puertas Moleculares\" De Aplicacion En Procesos De Reconocimiento Y Terapeutica Y Para La Deteccion De Explosivos/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//SAF2010-15512/ES/MECANISMOS MOLECULARES DE MODULADORES DE APOPTOSIS/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//MAT2009-14564-C04-03/ES/Sensores Y Remediadores De Agentes Nerviosos Y Simulantes/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/Generalitat Valenciana//PROMETEO09%2F2009%2F016/ES/Ayuda prometeo 2009 para el grupo de diseño y desarrollo de sensores/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//MAT2009-14564-C04-02/ES/Aproximacion Al Biomimetismo Usando Lenguas Electronicas Y Narices Para La Deteccion De Explosivos Y Agentes Nerviosos/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F005/ES/Identificación de nuevas dianas terapéuticas en angiogénesis y apoptosis basadas en interacciones proteína-proteína/ | |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic | es_ES |
dc.description.bibliographicCitation | Candel Busquets, I.; Aznar Gimeno, E.; Mondragón Martínez, L.; De La Torre Paredes, C.; Martínez Mañez, R.; Sancenón Galarza, F.; Marcos Martínez, MD.... (2012). Amidase-responsive controlled release of antitumoral drug into intracellular media using gluconamide-capped mesoporous silica nanoparticles. Nanoscale. 4(22):7237-7245. https://doi.org/10.1039/c2nr32062b | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1039/c2nr32062b | es_ES |
dc.description.upvformatpinicio | 7237 | es_ES |
dc.description.upvformatpfin | 7245 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 4 | es_ES |
dc.description.issue | 22 | es_ES |
dc.relation.senia | 230314 | |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |
dc.contributor.funder | Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina | es_ES |