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An Extracellular Subtilase Switch for Immune Priming in Arabidopsis

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An Extracellular Subtilase Switch for Immune Priming in Arabidopsis

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dc.contributor.author Ramirez Garcia, Vicente es_ES
dc.contributor.author López Sánchez, Ana es_ES
dc.contributor.author Mauch-Mani, Brigitte es_ES
dc.contributor.author Gil Morrió, María José es_ES
dc.contributor.author Vera Vera, Pablo es_ES
dc.date.accessioned 2014-09-01T10:22:42Z
dc.date.available 2014-09-01T10:22:42Z
dc.date.issued 2013-06
dc.identifier.issn 1553-7366
dc.identifier.uri http://hdl.handle.net/10251/39317
dc.description.abstract In higher eukaryotes, induced resistance associates with acquisition of a priming state of the cells for a more effective activation of innate immunity; however, the nature of the components for mounting this type of immunological memory is not well known. We identified an extracellular subtilase from Arabidopsis, SBT3.3, the overexpression of which enhances innate immune responses while the loss of function compromises them. SBT3.3 expression initiates a durable autoinduction mechanism that promotes chromatin remodeling and activates a salicylic acid(SA)-dependent mechanism of priming of defense genes for amplified response. Moreover, SBT3.3 expression-sensitized plants for enhanced expression of the OXI1 kinase gene and activation of MAP kinases following pathogen attack, providing additional clues for the regulation of immune priming by SBT3.3. Conversely, in sbt3.3 mutant plants pathogen-mediated induction of SA-related defense gene expression is drastically reduced and activation of MAP kinases inhibited. Moreover, chromatin remodeling of defense-related genes normally associated with activation of an immune priming response appear inhibited in sbt3.3 plants, further indicating the importance of the extracellular SBT3.3 subtilase in the establishment of immune priming. Our results also point to an epigenetic control in the regulation of plant immunity, since SBT3.3 is up-regulated and priming activated when epigenetic control is impeded. SBT3.3 represents a new regulator of primed immunity. es_ES
dc.description.sponsorship The Spanish MICINN (BFU2009-09771, EUI2009-04009 to PV) and Generalitat Valenciana (Prometeo2010/020 to PV) provided support for this work. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS Pathogens es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Systemic acquired-resistance es_ES
dc.subject Apoplastic oxidative burst es_ES
dc.subject Pattern-triggered immunity es_ES
dc.subject Beta-aminobutyric acid es_ES
dc.subject Pseudomonas-syringae es_ES
dc.subject Plant protease es_ES
dc.subject Tomato plants es_ES
dc.subject Defense es_ES
dc.subject Gene es_ES
dc.subject Expression es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title An Extracellular Subtilase Switch for Immune Priming in Arabidopsis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.ppat.1003445
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2009-09771/ES/Mecanismos De Control De La Resistencia%2FSusceptibilidad A Patogenos En Arabidopsis/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//EUI2009-04009/ES/IDENTIFICATION OF NEW PLANT SUSCEPTIBILITY FACTORS WHOSE MODIFICATION WOULD CONFER VIRUS RESISTANCE/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F020/ES/Identificación de nuevos fármacos con potencia potencial uso biotecnológico en cultivos mediante un abordaje de genética química/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Ramirez Garcia, V.; López Sánchez, A.; Mauch-Mani, B.; Gil Morrió, MJ.; Vera Vera, P. (2013). An Extracellular Subtilase Switch for Immune Priming in Arabidopsis. PLoS Pathogens. 9(6):1003445-1003445. https://doi.org/10.1371/journal.ppat.1003445 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1371/journal.ppat.1003445 es_ES
dc.description.upvformatpinicio 1003445 es_ES
dc.description.upvformatpfin 1003445 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 9 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 259920
dc.identifier.pmid 23818851 en_EN
dc.identifier.pmcid PMC3688555 en_EN
dc.contributor.funder Generalitat Valenciana es_ES
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