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HRS1 Acts as a Negative Regulator of Abscisic Acid Signaling to Promote Timely Germination of Arabidopsis Seeds

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HRS1 Acts as a Negative Regulator of Abscisic Acid Signaling to Promote Timely Germination of Arabidopsis Seeds

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dc.contributor.author Wu, Chongming es_ES
dc.contributor.author Feng, Juanjuan es_ES
dc.contributor.author Wang, Ran es_ES
dc.contributor.author Liu, Hong es_ES
dc.contributor.author Yang, Huixia es_ES
dc.contributor.author Rodríguez Egea, Pedro Luís es_ES
dc.contributor.author Qin, Huanju es_ES
dc.contributor.author Liu, Xin es_ES
dc.contributor.author Wang, Daowen es_ES
dc.date.accessioned 2014-12-10T18:41:09Z
dc.date.available 2014-12-10T18:41:09Z
dc.date.issued 2012-04-24
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10251/45323
dc.description.abstract In this work, we conducted functional analysis of Arabidopsis HRS1 gene in order to provide new insights into the mechanisms governing seed germination. Compared with wild type (WT) control, HRS1 knockout mutant (hrs1-1) exhibited significant germination delays on either normal medium or those supplemented with abscisic acid (ABA) or sodium chloride (NaCl), with the magnitude of the delay being substantially larger on the latter media. The hypersensitivity of hrs1-1 germination to ABA and NaCl required ABI3, ABI4 and ABI5, and was aggravated in the double mutant hrs1-1abi1-2 and triple mutant hrs1-1hab1-1abi1-2, indicating that HRS1 acts as a negative regulator of ABA signaling during seed germination. Consistent with this notion, HRS1 expression was found in the embryo axis, and was regulated both temporally and spatially, during seed germination. Further analysis showed that the delay of hrs1-1 germination under normal conditions was associated with reduction in the elongation of the cells located in the lower hypocotyl (LH) and transition zone (TZ) of embryo axis. Interestingly, the germination rate of hrs1-1 was more severely reduced by the inhibitor of cell elongation, and more significantly decreased by the suppressors of plasmalemma H+-ATPase activity, than that of WT control. The plasmalemma H+-ATPase activity in the germinating seeds of hrs1-1 was substantially lower than that exhibited by WT control, and fusicoccin, an activator of this pump, corrected the transient germination delay of hrs1-1. Together, our data suggest that HRS1 may be needed for suppressing ABA signaling in germinating embryo axis, which promotes the timely germination of Arabidopsis seeds probably by facilitating the proper function of plasmalemma H+-ATPase and the efficient elongation of LH and TZ cells. es_ES
dc.description.sponsorship This work was supported by a grant from the National Natural Science Foundation of China (30821061). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS ONE es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject MEMBRANE H+-ATPASE es_ES
dc.subject PROTEIN PHOSPHATASE 2C es_ES
dc.subject TRANSCRIPTION FACTOR es_ES
dc.subject ELONGATION GROWTH es_ES
dc.subject ROOT-GROWTH es_ES
dc.subject THALIANA es_ES
dc.subject ABA es_ES
dc.subject DORMANCY es_ES
dc.subject INHIBITION es_ES
dc.subject GENE es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title HRS1 Acts as a Negative Regulator of Abscisic Acid Signaling to Promote Timely Germination of Arabidopsis Seeds es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.pone.0035764
dc.relation.projectID info:eu-repo/grantAgreement/NSFC//30821061/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Wu, C.; Feng, J.; Wang, R.; Liu, H.; Yang, H.; Rodríguez Egea, PL.; Qin, H.... (2012). HRS1 Acts as a Negative Regulator of Abscisic Acid Signaling to Promote Timely Germination of Arabidopsis Seeds. PLoS ONE. 7(4):1-13. https://doi.org/10.1371/journal.pone.0035764 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1371/journal.pone.0035764 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 13 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 232900
dc.identifier.pmid 22545134 en_EN
dc.identifier.pmcid PMC3335793 en_EN
dc.contributor.funder National Natural Science Foundation of China es_ES
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