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Fibronectin adsorption and cell response on electroactive poly (vinylidene fluoride) films

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Fibronectin adsorption and cell response on electroactive poly (vinylidene fluoride) films

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dc.contributor.author Ribeiro, Clarisse es_ES
dc.contributor.author Panadero Pérez, Juan Alberto es_ES
dc.contributor.author Sencadas, Vitor Joao Gomes Da Silva es_ES
dc.contributor.author Lanceros Mendez, Senen es_ES
dc.contributor.author Tamaño Machiavello, María Noel es_ES
dc.contributor.author Moratal Pérez, David es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.date.accessioned 2015-03-12T11:05:15Z
dc.date.available 2015-03-12T11:05:15Z
dc.date.issued 2012
dc.identifier.issn 1748-6041
dc.identifier.uri http://hdl.handle.net/10251/48022
dc.description.abstract Due to the large potential of electroactive materials in novel tissue engineering strategies, the aim of this work is to determine if the crystalline phase and/or the surface electrical charge of electroactive poly(vinylidene fluoride), PVDF, have influence on the biological response in monolayer cell culture. Non-polar α-PVDF and electroactive β-PVDF were prepared. The β-PVDF films were poled by corona discharge to show negative or positive electrical surface charge density. It has been concluded that hydrophilicity of the PVDF substrates depends significantly on crystalline phase and polarity. Furthermore, by means of atomic force microscopy and an enzyme-linked immunosorbent assay test, it has been shown that positive or negative poling strongly influences the behavior of β-PVDF supports with respect to fibronectin (FN) adsorption, varying the exhibition of adhesion ligands of adsorbed FN. Culture of MC3T3-E1 pre-osteoeblasts proved that cell proliferation depends on surface polarity as well. These results open the viability of cell culture stimulation by mechanical deformation of a piezoelectric substrate that results in varying electrical charge densities on the substrate surface. es_ES
dc.description.sponsorship This work is funded by FEDER funds through the 'Programa Operacional Factores de Competitividade-COMPETE' and by national funds by FCT-Fundacao para a Ciencia e a Tecnologia, project reference NANO/NMed-SD/0156/2007. CR thanks the INL for a PhD grant. VS and JAP thank the FCT for the SFRH/BPD/63148/2009 and SFRH/BD/64586/2009/grants, respectively. JLGR acknowledges the support of the Spanish Ministry of Education through project no MAT2010-21611-C03-01 (including the FEDER financial support), project EUI2008-00126 and funding in the Centro de Investigacion Principe Felipe in the field of Regenerative Medicine through the collaboration agreement from the Conselleria de Sanidad (Generalitat Valenciana) and the Instituto de Salud Carlos III (Ministry of Science and Innovation). The authors thank Armando Ferreira for the help with the contact angle measurements and CENTI, Portugal, for allowing the use of the set-up. en_EN
dc.language Inglés es_ES
dc.publisher IOP Publishing: Hybrid Open Access es_ES
dc.relation.ispartof Biomedical Materials es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Poly(vinylidene fluoride); es_ES
dc.subject Fibronectin; es_ES
dc.subject Piezoelectric es_ES
dc.subject MC3T3-E1 preosteoeblasts es_ES
dc.subject Soft matter es_ES
dc.subject Liquids es_ES
dc.subject Polymers es_ES
dc.subject Medical physics es_ES
dc.subject Biological physics es_ES
dc.subject Chemical physics es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title Fibronectin adsorption and cell response on electroactive poly (vinylidene fluoride) films es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1088/1748-6041/7/3/035004
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//MAT2010-21611-C03-01/ES/MATERIALES BIOESTABLES Y BIOREABSORBIBLES A LARGO PLAZO COMO SOPORTES MACROPOROSOS PARA LA REGENERACION DEL CARTILAGO ARTICULAR/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F63148%2F2009/PT/ELECTROACTIVE MATERIALS BASED POROUS MEMBRANES AND SCAFFOLDS FOR BIOMEDICAL APPLICATIONS/
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//EUI2008-00126/ES/SMART JOINT IMPLANTS USING BIONANOCOMPOSITES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT/5876-PPCDTI/109368/PT/“Smart joint implants using bionanocomposites-(SIMBIO)”/
dc.relation.projectID info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F64586%2F2009/PT/ACTIVE BIOMATERIALS FOR CELL CULTURE UNDER MECHANICAL STIMULUS APPLIED TO CARTILAGE TISSUE ENGINEERING/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.description.bibliographicCitation Ribeiro, C.; Panadero Pérez, JA.; Sencadas, VJGDS.; Lanceros Mendez, S.; Tamaño Machiavello, MN.; Moratal Pérez, D.; Salmerón Sánchez, M.... (2012). Fibronectin adsorption and cell response on electroactive poly (vinylidene fluoride) films. Biomedical Materials. 7(3):35004-35014. https://doi.org/10.1088/1748-6041/7/3/035004 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1088/1748-6041/7/3/035004 es_ES
dc.description.upvformatpinicio 35004 es_ES
dc.description.upvformatpfin 35014 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 234720
dc.identifier.eissn 1748-605X
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Fundação para a Ciência e a Tecnologia, Portugal
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder European Regional Development Fund es_ES
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