Resumen:
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[ES] La vitrificación es una técnica de conservación de embriones, que hace posible la evaluación del efecto de la selección genética en la ganancia media diaria de peso, comparando poblaciones de la misma línea que se ...[+]
[ES] La vitrificación es una técnica de conservación de embriones, que hace posible la evaluación del efecto de la selección genética en la ganancia media diaria de peso, comparando poblaciones de la misma línea que se encuentran separadas en el tiempo, mediante la reconstitución de poblaciones. El objetivo de este estudio es reconstituir dos poblaciones de la línea Rosa de la Granja de Mejora Genética de la UPV, seleccionada por velocidad de crecimiento: una población control, de la cual se vitrificaron embriones de 72 horas en el año 1999 cuando se encontraba en la generación 25 y una población actual con embriones vitrificados también a 72 horas en 2014 encontrándose en la generación 36. Para ello se transferirán los embriones de cada una de las dos generaciones a 25 hembras de la línea Amarilla por tener estas mejores aptitudes maternales. Con esto se pretende obtener al menos quince orígenes vía macho de cada población para garantizar una baja consanguinidad en los posteriores cruces. A partir de los diez días de gestación, se realizarán ecografías cada dos días para llevar un seguimiento del crecimiento intrauterino de los fetos. A los catorce días se realizará una endoscopia para ver el número de implantados y posibles regresiones. Finalmente, los gazapos nacidos serán identificados con chips electrónicos para el seguimiento semanal de ganancia de peso individual.
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[EN] From a long time ago, selection has been a tool used by the humanity to obtain offspring with
interesting features from a productive point of view, including both animal and vegetable
species. Currently, there are ...[+]
[EN] From a long time ago, selection has been a tool used by the humanity to obtain offspring with
interesting features from a productive point of view, including both animal and vegetable
species. Currently, there are breeding programs of many species that use different selection
methods to preserve the best individuals, in order to reproduce them and to be able to improve
the lines. Due to the high genetic value that individuals achieve after years of selection and the
possibility of its loss which could suppose a delay, banks of genetic material were established.
In these banks, embryos are vitrified and stored in liquid nitrogen to -196OC.
Therefore, this work has been carried out in order to evaluate the current vitrified embryos and
those vitrified and stored 15 years ago from a rabbit line selected by daily average gain in the
fattening period, of the Genetic Progress Unit in the UPV.
In this work a total of 534 embryos were used, from two different generations of R line (18 and
36). From these 534, 301 were used as control (generation 36) and the 233 remaining as
problem (generation 18). The results obtained showed that there were no significant differences
in the post native growth up to the weaning, since both generations showed parallel and
practically superposed growth curves. Nevertheless, there were significant differences in the
prenatal growth, being the fetuses from the generation 36 longer than the generation 18. There
were also differences in the time of storage of the embryos, since those from 18 generation,
vitrified in 1999 showed less availability than those from generation 36, vitrified in 2014 and
therefore a less cryopreservation efficiency. The above mentioned was favored by the loss of
two straws that exploded during the warming procedure.
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