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Changes in caecal microbiota and mucosal gene expression revealed new aspects of Epizootic rabbit Enteropathy

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Changes in caecal microbiota and mucosal gene expression revealed new aspects of Epizootic rabbit Enteropathy

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Nombre: Christine Bäuerl;Mª ...
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dc.contributor.author Bauerl, Christine es_ES
dc.contributor.author Collado Amores, María Carmen es_ES
dc.contributor.author Zúñiga Cabrera, Manuel es_ES
dc.contributor.author Blas Ferrer, Enrique es_ES
dc.contributor.author Perez Martinez, Gaspar es_ES
dc.date.accessioned 2016-01-13T11:26:26Z
dc.date.available 2016-01-13T11:26:26Z
dc.date.issued 2014-08
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10251/59799
dc.description.abstract Epizootic Rabbit Enteropathy (ERE) is a severe disease of unknown aetiology that mainly affects post-weaning animals. Its incidence can be prevented by antibiotic treatment suggesting that bacterial elements are crucial for the development of the disease. Microbial dynamics and host responses during the disease were studied. Cecal microbiota was characterized in three rabbit groups (ERE-affected, healthy and healthy pretreated with antibiotics), followed by transcriptional analysis of cytokines and mucins in the cecal mucosa and vermix by q-rtPCR. In healthy animals, cecal microbiota with or without antibiotic pretreatment was very similar and dominated by Alistipes and Ruminococcus. Proportions of both genera decreased in ERE rabbits whereas Bacteroides, Akkermansia and Rikenella increased, as well as Clostridium, c-Proteobacteria and other opportunistic and pathogenic species. The ERE group displayed remarkable dysbiosis and reduced taxonomic diversity. Transcription rate of mucins and inflammatory cytokines was very high in ERE rabbits, except IL-2, and its analysis revealed the existence of two clearly different gene expression patterns corresponding to Inflammatory and (mucin) Secretory Profiles. Furthermore, these profiles were associated to different bacterial species, suggesting that they may correspond to different stages of the disease. Other data obtained in this work reinforced the notion that ERE morbidity and mortality is possibly caused by an overgrowth of different pathogens in the gut of animals whose immune defence mechanisms seem not to be adequately responding. es_ES
dc.description.sponsorship This work was supported by the grants AGL2010-22211, AGL2006-07596 and by the Fun-C-Food CSD2007-00063 project from the Consolider-Ingenio programme, both from the Spanish Ministry of Science and Innovation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS ONE es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Epizootic Rabbit Enteropathy (ERE) es_ES
dc.subject Cecal microbiota es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.subject.classification NUTRICION Y BROMATOLOGIA es_ES
dc.title Changes in caecal microbiota and mucosal gene expression revealed new aspects of Epizootic rabbit Enteropathy es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.pone.0105707
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2010-22211/ES/MECANISMO DE ACCION PROBIOTICA EN CEPAS DEL GRUPO LACTOBACILLUS CASEI-PARACASEI%2FRHAMNOSUS: IDENTIFICACION DE MOLECULAS CLAVE MEDIANTE MODELOS IN VITRO./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MEC//AGL2006-07596/ES/DESARROLLO DE ESTRATEGIAS GLOBALES DE ALIMENTACION Y MANEJO PARA MEJORAR LA SALUD Y EL BIENESTAR EN LAS GRANJAS CUNICOLAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MEC//CSD2007-00063/ES/Nuevos Ingredientes de Alimentos Funcionales para Mejorar la Salud/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.description.bibliographicCitation Bauerl, C.; Collado Amores, MC.; Zúñiga Cabrera, M.; Blas Ferrer, E.; Perez Martinez, G. (2014). Changes in caecal microbiota and mucosal gene expression revealed new aspects of Epizootic rabbit Enteropathy. PLoS ONE. 9(8):1-12. https://doi.org/10.1371/journal.pone.0105707 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/105707. doi:10.1371/journal.pone.0105707 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 12 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 9 es_ES
dc.description.issue 8 es_ES
dc.relation.senia 287925 es_ES
dc.identifier.pmid 25147938 en_EN
dc.identifier.pmcid PMC4141808 en_EN
dc.contributor.funder Ministerio de Educación y Ciencia es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
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