Vicente-Dolera, N.; Troadec, C.; Moya, M.; Río-Celestino, MD.; Pomares-Viciana, T.; Bendahmane, A.; Picó Sirvent, MB.... (2014). First TILLING Platform in Cucurbita pepo: A New Mutant Resource for Gene Function and Crop Improvement. PLoS ONE. 9(11):112743-112743. doi:10.1371/journal.pone.0112743
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/59819
Title:
|
First TILLING Platform in Cucurbita pepo: A New Mutant Resource for Gene Function and Crop Improvement
|
Author:
|
Vicente-Dolera, Nelly
Troadec, Christelle
Moya, Manuel
Río-Celestino, Mercedes del
Pomares-Viciana, Teresa
Bendahmane, Abdelhafid
Picó Sirvent, María Belén
Román, Belén
Gómez, Pedro
|
UPV Unit:
|
Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana
|
Issued date:
|
|
Abstract:
|
Although the availability of genetic and genomic resources for Cucurbita pepo has increased significantly, functional genomic resources are still limited for this crop. In this direction, we have developed a high throughput ...[+]
Although the availability of genetic and genomic resources for Cucurbita pepo has increased significantly, functional genomic resources are still limited for this crop. In this direction, we have developed a high throughput reverse genetic tool: the first TILLING (Targeting Induced Local Lesions IN Genomes) resource for this species. Additionally, we have used this resource to demonstrate that the previous EMS mutant population we developed has the highest mutation density compared with other cucurbits mutant populations. The overall mutation density in this first C. pepo TILLING platform was estimated to be 1/133 Kb by screening five additional genes. In total, 58 mutations confirmed by sequencing were identified in the five targeted genes, thirteen of which were predicted to have an impact on the function of the protein. The genotype/phenotype correlation was studied in a peroxidase gene, revealing that the phenotype of seedling homozygous for one of the isolated mutant alleles was albino. These results indicate that the TILLING approach in this species was successful at providing new mutations and can address the major challenge of linking sequence information to biological function and also the identification of novel variation for crop breeding.
[-]
|
Subjects:
|
Induced point mutations
,
Reverse genetics
,
Arabidopsis Thaliana
,
Genomics
,
Wheat
,
Population
,
Peroxidase
|
Copyrigths:
|
Reconocimiento (by)
|
Source:
|
PLoS ONE. (issn:
1932-6203
)
|
DOI:
|
10.1371/journal.pone.0112743
|
Publisher:
|
Public Library of Science
|
Publisher version:
|
http://dx.doi.org/10.1371/journal.pone.0112743
|
Project ID:
|
Spanish Project INIA (Instituto Nacional de Investigación y Tecnología Agraría y Almentaria) RTA2011-00044- C02-01
ANR MELODY (ANR-11-BSV7-0024)
European Research Council (ERCSEXYPARTH)
FEDER
FSE
|
Thanks:
|
Financial support was provided by the Spanish Project INIA (Instituto Nacional de Investigacion y Tecnologia Agraria y Almentaria) RTA2011-00044C02-01, the ANR MELODY (ANR-11-BSV7-0024), the European Research Council ...[+]
Financial support was provided by the Spanish Project INIA (Instituto Nacional de Investigacion y Tecnologia Agraria y Almentaria) RTA2011-00044C02-01, the ANR MELODY (ANR-11-BSV7-0024), the European Research Council (ERCSEXYPARTH), FEDER, and FSE funds. NVD has been awarded a grant by the Andalusian Institute of Agronomy Research IFAPA. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
[-]
|
Type:
|
Artículo
|