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First TILLING Platform in Cucurbita pepo: A New Mutant Resource for Gene Function and Crop Improvement

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First TILLING Platform in Cucurbita pepo: A New Mutant Resource for Gene Function and Crop Improvement

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dc.contributor.author Vicente-Dolera, Nelly es_ES
dc.contributor.author Troadec, Christelle es_ES
dc.contributor.author Moya, Manuel es_ES
dc.contributor.author Río-Celestino, Mercedes del es_ES
dc.contributor.author Pomares-Viciana, Teresa es_ES
dc.contributor.author Bendahmane, Abdelhafid es_ES
dc.contributor.author Picó Sirvent, María Belén es_ES
dc.contributor.author Román, Belén es_ES
dc.contributor.author Gómez, Pedro es_ES
dc.date.accessioned 2016-01-13T12:28:28Z
dc.date.available 2016-01-13T12:28:28Z
dc.date.issued 2014-11
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10251/59819
dc.description.abstract Although the availability of genetic and genomic resources for Cucurbita pepo has increased significantly, functional genomic resources are still limited for this crop. In this direction, we have developed a high throughput reverse genetic tool: the first TILLING (Targeting Induced Local Lesions IN Genomes) resource for this species. Additionally, we have used this resource to demonstrate that the previous EMS mutant population we developed has the highest mutation density compared with other cucurbits mutant populations. The overall mutation density in this first C. pepo TILLING platform was estimated to be 1/133 Kb by screening five additional genes. In total, 58 mutations confirmed by sequencing were identified in the five targeted genes, thirteen of which were predicted to have an impact on the function of the protein. The genotype/phenotype correlation was studied in a peroxidase gene, revealing that the phenotype of seedling homozygous for one of the isolated mutant alleles was albino. These results indicate that the TILLING approach in this species was successful at providing new mutations and can address the major challenge of linking sequence information to biological function and also the identification of novel variation for crop breeding. es_ES
dc.description.sponsorship Financial support was provided by the Spanish Project INIA (Instituto Nacional de Investigacion y Tecnologia Agraria y Almentaria) RTA2011-00044C02-01, the ANR MELODY (ANR-11-BSV7-0024), the European Research Council (ERCSEXYPARTH), FEDER, and FSE funds. NVD has been awarded a grant by the Andalusian Institute of Agronomy Research IFAPA. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS ONE es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Induced point mutations es_ES
dc.subject Reverse genetics es_ES
dc.subject Arabidopsis Thaliana es_ES
dc.subject Genomics es_ES
dc.subject Wheat es_ES
dc.subject Population es_ES
dc.subject Peroxidase es_ES
dc.subject.classification GENETICA es_ES
dc.title First TILLING Platform in Cucurbita pepo: A New Mutant Resource for Gene Function and Crop Improvement es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.pone.0112743
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RTA2011-00044-C02-01/ES/Desarrollo competitivo de nuevos cultivares de calabacín y ampliación de la plataforma genómica para la mejora de esta hortaliza/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ANR//ANR-11-BSV7-0024/FR/Plant sex determination: functional characterization of the monoecy and gynoecy genes in Cucurbitaceae/MELODY/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Vicente-Dolera, N.; Troadec, C.; Moya, M.; Río-Celestino, MD.; Pomares-Viciana, T.; Bendahmane, A.; Picó Sirvent, MB.... (2014). First TILLING Platform in Cucurbita pepo: A New Mutant Resource for Gene Function and Crop Improvement. PLoS ONE. 9(11):112743-112743. https://doi.org/10.1371/journal.pone.0112743 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1371/journal.pone.0112743 es_ES
dc.description.upvformatpinicio 112743 es_ES
dc.description.upvformatpfin 112743 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 9 es_ES
dc.description.issue 11 es_ES
dc.relation.senia 278474 es_ES
dc.identifier.pmid 25386735 en_EN
dc.identifier.pmcid PMC4227871 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Agence Nationale de la Recherche, Francia es_ES
dc.contributor.funder European Social Fund es_ES
dc.contributor.funder European Regional Development Fund es_ES
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