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Use of Chemometric techniques to design a microbiological method for sulfonamide detection in milk

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Use of Chemometric techniques to design a microbiological method for sulfonamide detection in milk

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dc.contributor.author Nagel, Orlando Guillermo es_ES
dc.contributor.author Molina Pons, Mª Pilar es_ES
dc.contributor.author Althaus Bellon, Rafael Lisandro es_ES
dc.date.accessioned 2016-01-27T12:10:46Z
dc.date.available 2016-01-27T12:10:46Z
dc.date.issued 2013
dc.identifier.issn 1212-1800
dc.identifier.uri http://hdl.handle.net/10251/60266
dc.description.abstract [EN]We proposed an experimental design of a microbial bioassay of dichotomous response (positive or negative) using Bacillus subtilis BGA for the detection of sulfonamide residues. In the first stage, the bioassay response time was reduced to 6 h by increasing the spore concentration of B. subtilis. Then, the effects of spore, indicator, trimethoprim (TMP) concentration, and volume of the culture medium were examined with a Plackett Burman design (2(4-1)). Finally, the effect of TMP concentration on the method detection capabilities and specificity was analysed using a logistic model with interaction. The detection capabilities of sulfonamides in milk are close to the MRLs when using 500 mu g/1 of TMP in the culture medium of the bioassay. It is concluded that the experimental design techniques and a logistic regression model can be used to design successfully a dichotomous response bioassay. es_ES
dc.description.sponsorship Partly supported by the Universidad Nacional del Litoral (Santa Fe, República Argentina), Project CAI+D’09/12, No. 033-173 Res. H.C.D. No. 100/09.
dc.language Inglés es_ES
dc.publisher Czech Academy of Agricultural Sciences es_ES
dc.relation UNL/033-173 100/09 es_ES
dc.relation.ispartof Czech Journal of Food Sciences es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject B. subtilis es_ES
dc.subject Microbial inhibition method es_ES
dc.subject Bioassay es_ES
dc.subject Experimental design es_ES
dc.subject Logistic regression model es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Use of Chemometric techniques to design a microbiological method for sulfonamide detection in milk es_ES
dc.type Artículo es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Nagel, OG.; Molina Pons, MP.; Althaus Bellon, RL. (2013). Use of Chemometric techniques to design a microbiological method for sulfonamide detection in milk. Czech Journal of Food Sciences. 31(6):627-632. http://hdl.handle.net/10251/60266 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://www.agriculturejournals.cz/web/cjfs/ es_ES
dc.description.upvformatpinicio 627 es_ES
dc.description.upvformatpfin 632 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 31 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 254973 es_ES
dc.contributor.funder Universidad Nacional del Litoral, Argentina


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