Corbatón Báguena, MJ.; Gugliuzza, A.; Cassano, A.; Mazzei, R.; Giorno, L. (2015). Destabilization and removal of immobilized enzymes adsorbed onto polyethersulfone ultrafiltration membranes by salt solutions. Journal of Membrane Science. 486:207-214. https://doi.org/10.1016/j.memsci.2015.03.061
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/60347
Title:
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Destabilization and removal of immobilized enzymes adsorbed onto polyethersulfone ultrafiltration membranes by salt solutions
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Author:
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Corbatón Báguena, María José
Gugliuzza, A.
Cassano, A.
Mazzei, R.
Giorno, L.
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UPV Unit:
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Universitat Politècnica de València. Departamento de Ingeniería Química y Nuclear - Departament d'Enginyeria Química i Nuclear
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Issued date:
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Abstract:
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In this work the effectiveness of two saline solutions (NaCl and Na2SO4) to clean a permanently hydrophilic polyethersulfone (PESH) ultrafiltration (UF) membrane with a molecular weight cut-off (MWCO) of 30 kDa previously ...[+]
In this work the effectiveness of two saline solutions (NaCl and Na2SO4) to clean a permanently hydrophilic polyethersulfone (PESH) ultrafiltration (UF) membrane with a molecular weight cut-off (MWCO) of 30 kDa previously fouled with enzymatic solutions was investigated. The influence of protein concentration in the enzymatic solution during the fouling step and the effect of salt type during the cleaning procedure were studied.
The protein aggregation was analyzed in solution and onto the membrane surface by using several techniques including Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM) and Infrared Spectroscopy with Attenuated Total Reflectance (ATR-FTIR). In addition, mechanisms that dominate membrane fouling were studied by fitting some mathematical models (Hermia's models adapted to crossflow filtration, a combined model based on the complete blocking and cake formation equations and a resistance-in-series model) to the experimental data.
Fouling results showed that the complete blocking/adsorption on membrane surface was the predominant fouling mechanism. Regarding the cleaning results, higher cleaning efficiency and low residual protein concentration was obtained with NaCl solutions for all the feed solutions tested due to the favorable interaction between Cl and proteins.
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Subjects:
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Membrane cleaning
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Protein fouling
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Salt solutions
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Ultrafiltration
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Mathematical models
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Copyrigths:
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Reserva de todos los derechos
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Source:
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Journal of Membrane Science. (issn:
0376-7388
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DOI:
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10.1016/j.memsci.2015.03.061
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Publisher:
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Elsevier
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Publisher version:
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http://dx.doi.org/10.1016/j.memsci.2015.03.061
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Project ID:
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info:eu-repo/grantAgreement/MICINN//CTM2010-20186/ES/TECNICAS NO CONVENCIONALES PARA LA LIMPIEZA DE MEMBRANAS DE ULTRAFILTRACION APLICADAS EN LA INDUSTRIA ALIMENTARIA/ /
info:eu-repo/grantAgreement/MiSE//PON01_01545/IT/Sistemi tecnologici avanzati e processi integrati nella filiera olivicola per la valorizzazione dei prodotti e dei sottoprodotti, lo sviluppo di nuovi settori e la creazione di sistemi produttivi eco-compatibili/
info:eu-repo/grantAgreement/MINECO//EEBB-I-14-09011/ES/EEBB-I-14-09011/
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Thanks:
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Maria-Jose Corbaton-Baguena wishes to gratefully acknowledge the financial support from the Spanish Ministry of Economy and Competitiveness through the grant EEBB-I-14-09011 (project CTM2010-20186). The authors acknowledge ...[+]
Maria-Jose Corbaton-Baguena wishes to gratefully acknowledge the financial support from the Spanish Ministry of Economy and Competitiveness through the grant EEBB-I-14-09011 (project CTM2010-20186). The authors acknowledge the European Union, Fondo Europeo di Sviluppo Regionale, The Ministero dell'Istruzione, dell'Universita e della Ricerca - MIUR, The Ministero dello Svilupppo Economic - MSE - for the financial support to the project "Sistemi tecnologici avanzati e processi integrati della filiera olivicola per la valorizzazione dei prodotti e dei sottoprodotti, lo sviluppo di nuovi settori e la creazione di sistemi produttivi Eco-compatibili" (PON Olio Piu, PON01_01545), within the framework PON Ricerca e Competitivita 2007-2013.
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Type:
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Artículo
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