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Electronic excited states of guanine-cytosine hairpins and duplexes studied by fluorescence spectroscopy

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Electronic excited states of guanine-cytosine hairpins and duplexes studied by fluorescence spectroscopy

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dc.contributor.author Brazard, J. es_ES
dc.contributor.author Thazhathveetil, A.K. es_ES
dc.contributor.author Vayá Pérez, Ignacio es_ES
dc.contributor.author Lewis, F.D. es_ES
dc.contributor.author Gustavsson, T. es_ES
dc.contributor.author Markovitsi, D. es_ES
dc.date.accessioned 2016-01-29T11:45:22Z
dc.date.available 2016-01-29T11:45:22Z
dc.date.issued 2013
dc.identifier.issn 1474-905X
dc.identifier.uri http://hdl.handle.net/10251/60361
dc.description.abstract [EN] Guanine-cytosine hairpins, containing a hexaethylene glycol bridge, are studied by steady-state fluorescence spectroscopy and time-correlated single photon counting; their properties are compared to those of duplexes with the same sequence. It is shown that, both in hairpins and in duplexes, base pairing induces quenching of the pi pi(star) fluorescence, the quantum yield decreasing by at least two orders of magnitude. When the size of the systems increases from two to ten base pairs, a fluorescent component decaying on the nanosecond time-scale appears at energy higher than that stemming from the bright states of non-interacting mono-nucleotides (ca. 330 nm). For ten base pairs, this new fluorescence forms a well-defined band peaking at 305 nm. Its intensity is about 20% higher for the hairpin compared to the duplex. Its position (red-shifted by 1600 cm(-1)) and width (broader by 1800 cm(-1) FWHM) differ from those observed for large duplexes containing 1000 base pairs, suggesting the involvement of electronic coupling. Fluorescence anisotropy reveals that the excited states responsible for high energy emission are not populated directly upon photon absorption but are reached during a relaxation process. They are assigned to charge transfer states. According to the emerging picture, the amplitude of conformational motions determines whether instantaneous deactivation to the ground state or emission from charge transfer states will take place, while pi pi(star) fluorescence is associated to imperfect base-pairing. es_ES
dc.description.sponsorship The French Agency for Research (ANR-10-BLAN-0809-01), the Conselleria de Educacion-Generalitat Valenciana (VALi+D program to I.V., N° 2010033) and the US Department of Energy, Office of Basic Energy Sciences (grant no. DE-FG02-96ER14604 to F.D.L.) are acknowledged for financial support.
dc.language Inglés es_ES
dc.publisher Royal Society of Chemistry es_ES
dc.relation.ispartof Photochemical & Photobiological Sciences Photochemical and Photobiological Sciences es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.subject.classification QUIMICA ORGANICA es_ES
dc.title Electronic excited states of guanine-cytosine hairpins and duplexes studied by fluorescence spectroscopy es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1039/c3pp50088h
dc.relation.projectID info:eu-repo/grantAgreement/ANR//ANR-10-BLAN-0809/FR/Photo-induced energy transfer in methylated DNA helices and its relevance to UV damage : an interactive theoretical-experimental study/DNAexciton/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//APOSTD%2F2010%2F033/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/DOE//DE-FG02-96ER14604/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Brazard, J.; Thazhathveetil, A.; Vayá Pérez, I.; Lewis, F.; Gustavsson, T.; Markovitsi, D. (2013). Electronic excited states of guanine-cytosine hairpins and duplexes studied by fluorescence spectroscopy. Photochemical & Photobiological Sciences Photochemical and Photobiological Sciences. 12(8):1453-1459. https://doi.org/10.1039/c3pp50088h es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1039/c3pp50088h es_ES
dc.description.upvformatpinicio 1453 es_ES
dc.description.upvformatpfin 1459 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 12 es_ES
dc.description.issue 8 es_ES
dc.relation.senia 254951 es_ES
dc.contributor.funder Agence Nationale de la Recherche, Francia
dc.contributor.funder Generalitat Valenciana
dc.contributor.funder U.S. Department of Energy
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