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Polymerase chain reaction detection of rabbit DNA in food and animal feed

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Polymerase chain reaction detection of rabbit DNA in food and animal feed

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dc.contributor.author Martín, I.
dc.contributor.author García, T.
dc.contributor.author Fajardo, V.
dc.contributor.author Rojas, M.
dc.contributor.author Pegels, N.
dc.contributor.author Hernández, P.E.
dc.contributor.author González, I.
dc.contributor.author Martín, R.
dc.date.accessioned 2009-09-03T07:54:09Z
dc.date.available 2009-09-03T07:54:09Z
dc.date.issued 2009
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/6084
dc.description.abstract [EN] A polymerase chain reaction (PCR) based on oligonucleotide primers targeting the mitochondrial 12S rRNA gene was developed for the specific identification of rabbit DNA (Oryctolagus cuniculus) in food and feedstuffs. The specificity of the primers was verified by PCR analysis of DNA from 32 non-target species including mammals, birds, fish, and plant species. Analysis of experimental mixtures demonstrated the presence of rabbit-derived materials in the range of 0.1-100%. Prolonged heat treatment (up to 133ºC for 20 min at 300 kPa) applied to rabbit muscle/oats binary mixtures did not affect the performance of the method, which could therefore be said to be very useful for the accurate identification of rabbit materials in products submitted to denaturing technologies when other methods are not suitable. en_EN
dc.description.sponsorship This study was supported by Grant Nº. AGL 2007-60077 from the Spanish Ministerio de Educación y Ciencia and the Programa de Vigilancia Sanitaria S-0505/AGR/000265 of the Comunidad de Madrid (Spain). Irene Martín is the recipient of a fellowship from the Universidad Complutense de Madrid (Spain). Violeta Fajardo and María Rojas are recipients of fellowships from the Ministerio de Educación y Ciencia (Spain).
dc.language Inglés en_EN
dc.publisher World Rabbit Science. ICTA. UPV en_EN
dc.relation.ispartof World Rabbit Science
dc.rights Reserva de todos los derechos es_ES
dc.subject 12s rrna gene en_EN
dc.subject Species identification en_EN
dc.subject Pcr en_EN
dc.subject Feedstuff en_EN
dc.subject Rabbit en_EN
dc.title Polymerase chain reaction detection of rabbit DNA in food and animal feed en_EN
dc.type Artículo en_EN
dc.identifier.doi 10.4995/wrs.2009.667
dc.relation.projectID info:eu-repo/grantAgreement/MEC//AGL2007-60077/ES/AUTENTIFICACION, POR TECNICAS DE PCR Y DE PCR EN TIEMPO REAL, DE CARNES Y PRODUCTOS CARNICOS TRANSFORMADOS PROCEDENTES DE AVES DE CAZA Y DE ESPECIES DE LA AVICULTURA ALTERNATIVA: TRAZABILIDAD/
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Martín, I.; García, T.; Fajardo, V.; Rojas, M.; Pegels, N.; Hernández, P.; González, I.... (2009). Polymerase chain reaction detection of rabbit DNA in food and animal feed. World Rabbit Science. 17(1):27-34. https://doi.org/10.4995/wrs.2009.667 es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2009.667 es_ES
dc.description.upvformatpinicio 27
dc.description.upvformatpfin 34
dc.description.volume 17
dc.description.issue 1
dc.identifier.eissn 1989-8886 es_ES
dc.contributor.funder Ministerio de Educación y Ciencia
dc.contributor.funder Comunidad de Madrid


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