Peiró Morell, A.; Izquierdo García, AC.; Sanchez Navarro, JA.; Pallás Benet, V.; Mulet Salort, JM.; Aparicio Herrero, F. (2014). Patellins 3 and 6, two members of the Plant Patellin family, interact with the movement protein of Alfalfa mosaic virus and interfere with viral movement. Molecular Plant Pathology. 15(9):881-891. https://doi.org/10.1111/mpp.12146
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/61427
Título:
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Patellins 3 and 6, two members of the Plant Patellin family, interact with the movement protein of Alfalfa mosaic virus and interfere with viral movement
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Autor:
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Peiró Morell, Ana
Izquierdo García, Ana Cristina
Sanchez Navarro, Jesus Angel
Pallás Benet, Vicente
Mulet Salort, José Miguel
Aparicio Herrero, Frederic
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Entidad UPV:
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Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
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Fecha difusión:
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Resumen:
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[EN] Movement proteins (MPs) encoded by plant viruses interact with host proteins to facilitate or interfere with intra- and/or intercellular viral movement. Using yeast two-hybrid and bimolecular fluorescence complementation ...[+]
[EN] Movement proteins (MPs) encoded by plant viruses interact with host proteins to facilitate or interfere with intra- and/or intercellular viral movement. Using yeast two-hybrid and bimolecular fluorescence complementation assays, we herein present invivo evidence for the interaction between Alfalfa mosaic virus (AMV) MP and Arabidopsis Patellin 3 (atPATL3) and Patellin 6 (atPATL6), two proteins containing a Sec14 domain. Proteins with Sec14 domains are implicated in membrane trafficking, cytoskeleton dynamics, lipid metabolism and lipid-mediated regulatory functions. Interestingly, the overexpression of atPATL3 and/or atPATL6 interfered with the plasmodesmata targeting of AMV MP and correlated with reduced infection foci size. Consistently, the viral RNA levels increased in the single and double Arabidopsis knockout mutants for atPATL3 and atPATL6. Our results indicate that, in general, MP-PATL interactions interfere with the correct subcellular targeting of MP, thus rendering the intracellular transport of viral MP-containing complexes less efficient and diminishing cell-to-cell movement.
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Palabras clave:
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Movement protein
,
Patellin
,
Intercellular movement
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AMV
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Ilarvirus
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Derechos de uso:
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Reserva de todos los derechos
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Fuente:
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Molecular Plant Pathology. (issn:
1464-6722
) (eissn:
1364-3703
)
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DOI:
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10.1111/mpp.12146
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Editorial:
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Wiley-Blackwell
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Versión del editor:
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http://dx.doi.org/10.1111/mpp.12146
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Código del Proyecto:
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info:eu-repo/grantAgreement/MICINN//BFU2008-00604/ES/REGULACION DE H+-ATPASA, TRANSPORTE DE K+ Y TOR: INVESTIGANDO LA RELACION ENTRE DETERMINANTES BIOFISICOS Y BIOQUIMICOS DEL CRECIMIENTO CELULAR EN ARABIDOPSIS/
info:eu-repo/grantAgreement/MICINN//RYC-2010-06169/ES/RYC-2010-06169/
info:eu-repo/grantAgreement/MICINN//BIO2011-25018/ES/TRAFICO INTRACELULAR, INTERCELULAR Y VASCULAR DE RNAS Y PROTEINAS VIRALES Y SUBVIRALES EN PLANTAS¿/
info:eu-repo/grantAgreement/GVA//PROMETEO%2F2011%2F003/
info:eu-repo/grantAgreement/UPV//PAID-06-10-1496/
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Descripción:
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This is the accepted version of the following article: Peiró Morell, A.; Izquierdo Garcia, AC.; Sanchez Navarro, JA.; Pallás Benet, V.; Mulet Salort, JM.; Aparicio Herrero, F. (2014). Patellins 3 and 6, two members of the Plant Patellin family, interact with the movement protein of Alfalfa mosaic virus and interfere with viral movement. Molecular Plant Pathology. 15(9):881-891. doi:10.1111/mpp.12146., which has been published in final form at http://dx.doi.org/10.1111/mpp.12146.
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Agradecimientos:
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AP was a recipient of a Pre-Doctoral Fellowship from the program JAE Pre-Doc of Consejo superior de Investigaciones Cientificas. ACI-G was a recipient of a Pre-Doctoral Fellowship associated with the project BFU2008-00604. ...[+]
AP was a recipient of a Pre-Doctoral Fellowship from the program JAE Pre-Doc of Consejo superior de Investigaciones Cientificas. ACI-G was a recipient of a Pre-Doctoral Fellowship associated with the project BFU2008-00604. FA was a recipient of a contract Ramon y Cajal (RYC-2010-06169) Program of the Ministerio de Educacion y Ciencia of Spain. We thank L. Corachan for excellent technical assistance. This work was supported by grants BIO2011-25018 from the Direccion General de Investigacion Cientifica y Tecnica, the Prometeo Program GV2011/003 from the Generalitat Valenciana and PAID-06-10-1496 from the Universitat Politecnica de Valencia (Spain).
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Tipo:
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Artículo
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