Berbegal Martinez, M.; Garzón, CD.; Ortega, A.; Armengol Fortí, J.; Jiménez Díaz, RM.; Jiménez Gasco, MDM. (2011). Development and application of new molecular markers for analysis of genetic diversity in Verticillium dahliae populations. Plant Pathology. 60(5):866-877. doi:10.1111/j.1365-3059.2011.02432.x
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/62066
Title:
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Development and application of new molecular markers for analysis of genetic diversity in Verticillium dahliae populations
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Author:
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Berbegal Martínez, Mónica
Garzón, C. D.
Ortega, A.
Armengol Fortí, Josep
Jiménez Díaz, R. M.
Jiménez Gasco, María del Mar
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UPV Unit:
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Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals
Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani
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Issued date:
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Abstract:
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The aim of this study was to develop new polymorphic markers for analysis of genetic diversity in the fungal soilborne plant pathogen Verticillium dahliae. Twelve polymorphic markers (five microsatellites and seven polymorphic ...[+]
The aim of this study was to develop new polymorphic markers for analysis of genetic diversity in the fungal soilborne plant pathogen Verticillium dahliae. Twelve polymorphic markers (five microsatellites and seven polymorphic sequences) were developed from a genomic library enriched for microsatellites. Screening of polymorphic loci was done using a collection of 25V. dahliae isolates of diverse geographic origins, host sources and vegetative compatibility groups (VCGs). Three methods were used to score alleles: polyacrylamide gel electrophoresis (PAGE), sequencing of PCR-amplified loci, and capillary electrophoresis. The new markers were used to assess genetic differentiation between isolates associated with different host plants. Two collections of isolates were analysed, obtained from artichoke (30 isolates) and potato (20 isolates) from crops grown in rotation located in the same area in eastern-central Spain. The resolution of genetic differentiation between these two collections using the new markers was compared to that provided by other often-used markers (SCARs and VCGs). Sequence analysis of the alleles proved to be the most unambiguous technique for scoring microsatellite data. The relatively high genetic differentiation observed between isolates from different crops (genetic differentiation coefficient, G ST=0·24) and their high genotypic diversity suggest a divergence between V. dahliae from artichoke and potato. It is hypothesized that evolution of V. dahliae from the local resident population in association with the two host crops has occurred. The new markers are useful for resolving population structure within V. dahliae and may contribute to a better understanding of the population biology of this fungus. © 2011 The Authors. Plant Pathology © 2011 BSPP.
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Subjects:
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Fungal ecology
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Host adaptation
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Molecular markers
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SSRs
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Verticillium wilt
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Allele
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Assessment method
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Crop rotation
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Data set
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Electrokinesis
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Fungus
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Gel
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Genetic differentiation
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Genetic marker
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Genomics
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Geographical region
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Host plant
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Molecular analysis
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Polymerase chain reaction
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Polymorphism
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Population structure
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Potato
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Resolution
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Spain
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Cynara scolymus
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Fungi
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Solanum tuberosum
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Verticillium dahliae
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Copyrigths:
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Cerrado |
Source:
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Plant Pathology. (issn:
0032-0862
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DOI:
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10.1111/j.1365-3059.2011.02432.x
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Publisher:
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Wiley
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Publisher version:
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http://dx.doi.org/10.1111/j.1365-3059.2011.02432.x
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Project ID:
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MEC/AP2002-3818
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Thanks:
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MB was a recipient of a FPU fellowship from the Ministry of Education and Science of Spain (AP2002-3818); we thank Jeniffer Yanez and Rachel Leonard for very valuable technical support and D. Davis for providing the V. ...[+]
MB was a recipient of a FPU fellowship from the Ministry of Education and Science of Spain (AP2002-3818); we thank Jeniffer Yanez and Rachel Leonard for very valuable technical support and D. Davis for providing the V. albo-atrum isolate. This research was partially supported by The Pennsylvania State University Agricultural Experiment Station and the Fundacion Ramon Areces grant 'Verticilosis del olivo' from Spain. Editorial improvement of the manuscript by anonymous reviewers and the editor is gratefully acknowledged.
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Type:
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Artículo
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