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Development and application of new molecular markers for analysis of genetic diversity in Verticillium dahliae populations

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Development and application of new molecular markers for analysis of genetic diversity in Verticillium dahliae populations

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dc.contributor.author Berbegal Martínez, Mónica es_ES
dc.contributor.author Garzón, C. D. es_ES
dc.contributor.author Ortega, A. es_ES
dc.contributor.author Armengol Fortí, Josep es_ES
dc.contributor.author Jiménez Díaz, R. M. es_ES
dc.contributor.author Jiménez Gasco, María del Mar es_ES
dc.date.accessioned 2016-03-29T14:35:56Z
dc.date.available 2016-03-29T14:35:56Z
dc.date.issued 2011-10
dc.identifier.issn 0032-0862
dc.identifier.uri http://hdl.handle.net/10251/62066
dc.description.abstract The aim of this study was to develop new polymorphic markers for analysis of genetic diversity in the fungal soilborne plant pathogen Verticillium dahliae. Twelve polymorphic markers (five microsatellites and seven polymorphic sequences) were developed from a genomic library enriched for microsatellites. Screening of polymorphic loci was done using a collection of 25V. dahliae isolates of diverse geographic origins, host sources and vegetative compatibility groups (VCGs). Three methods were used to score alleles: polyacrylamide gel electrophoresis (PAGE), sequencing of PCR-amplified loci, and capillary electrophoresis. The new markers were used to assess genetic differentiation between isolates associated with different host plants. Two collections of isolates were analysed, obtained from artichoke (30 isolates) and potato (20 isolates) from crops grown in rotation located in the same area in eastern-central Spain. The resolution of genetic differentiation between these two collections using the new markers was compared to that provided by other often-used markers (SCARs and VCGs). Sequence analysis of the alleles proved to be the most unambiguous technique for scoring microsatellite data. The relatively high genetic differentiation observed between isolates from different crops (genetic differentiation coefficient, G ST=0·24) and their high genotypic diversity suggest a divergence between V. dahliae from artichoke and potato. It is hypothesized that evolution of V. dahliae from the local resident population in association with the two host crops has occurred. The new markers are useful for resolving population structure within V. dahliae and may contribute to a better understanding of the population biology of this fungus. © 2011 The Authors. Plant Pathology © 2011 BSPP. es_ES
dc.description.sponsorship MB was a recipient of a FPU fellowship from the Ministry of Education and Science of Spain (AP2002-3818); we thank Jeniffer Yanez and Rachel Leonard for very valuable technical support and D. Davis for providing the V. albo-atrum isolate. This research was partially supported by The Pennsylvania State University Agricultural Experiment Station and the Fundacion Ramon Areces grant 'Verticilosis del olivo' from Spain. Editorial improvement of the manuscript by anonymous reviewers and the editor is gratefully acknowledged. en_EN
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation MEC/AP2002-3818
dc.relation.ispartof Plant Pathology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Fungal ecology es_ES
dc.subject Host adaptation es_ES
dc.subject Molecular markers es_ES
dc.subject SSRs es_ES
dc.subject Verticillium wilt es_ES
dc.subject Allele es_ES
dc.subject Assessment method es_ES
dc.subject Crop rotation es_ES
dc.subject Data set es_ES
dc.subject Electrokinesis es_ES
dc.subject Fungus es_ES
dc.subject Gel es_ES
dc.subject Genetic differentiation es_ES
dc.subject Genetic marker es_ES
dc.subject Genomics es_ES
dc.subject Geographical region es_ES
dc.subject Host plant es_ES
dc.subject Molecular analysis es_ES
dc.subject Polymerase chain reaction es_ES
dc.subject Polymorphism es_ES
dc.subject Population structure es_ES
dc.subject Potato es_ES
dc.subject Resolution es_ES
dc.subject Spain es_ES
dc.subject Cynara scolymus es_ES
dc.subject Fungi es_ES
dc.subject Solanum tuberosum es_ES
dc.subject Verticillium dahliae es_ES
dc.subject.classification BOTANICA es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.subject.classification ECOSISTEMAS AGROFORESTALES (UPV) es_ES
dc.title Development and application of new molecular markers for analysis of genetic diversity in Verticillium dahliae populations es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/j.1365-3059.2011.02432.x
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.description.bibliographicCitation Berbegal Martinez, M.; Garzón, CD.; Ortega, A.; Armengol Fortí, J.; Jiménez Díaz, RM.; Jiménez Gasco, MDM. (2011). Development and application of new molecular markers for analysis of genetic diversity in Verticillium dahliae populations. Plant Pathology. 60(5):866-877. doi:10.1111/j.1365-3059.2011.02432.x es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1111/j.1365-3059.2011.02432.x es_ES
dc.description.upvformatpinicio 866 es_ES
dc.description.upvformatpfin 877 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 60 es_ES
dc.description.issue 5 es_ES
dc.relation.senia 213773 es_ES
dc.contributor.funder Ministerio de Educación y Ciencia
dc.contributor.funder Pennsylvania State University Agricultural Experiment Station
dc.contributor.funder Fundación Ramón Areces


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