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Functional specialization of duplicated AP3-like genes in Medicago truncatula

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Functional specialization of duplicated AP3-like genes in Medicago truncatula

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dc.contributor.author Roque Mesa, Edelin Marta es_ES
dc.contributor.author Serwatowska, Joanna es_ES
dc.contributor.author Rochina Peñalver, Mª Cruz es_ES
dc.contributor.author Wen, Jiangqi es_ES
dc.contributor.author Mysore, Kirankumar S. es_ES
dc.contributor.author Yenush, Lynne es_ES
dc.contributor.author Beltran Porter, Jose Pio es_ES
dc.contributor.author Cañas Clemente, Luís Antonio es_ES
dc.date.accessioned 2016-04-06T11:36:15Z
dc.date.available 2016-04-06T11:36:15Z
dc.date.issued 2013-02
dc.identifier.issn 0960-7412
dc.identifier.uri http://hdl.handle.net/10251/62292
dc.description This is the accepted version of the following article: Roque, E., Serwatowska, J., Cruz Rochina, M., Wen, J., Mysore, K. S., Yenush, L., Beltrán, J. P. and Cañas, L. A. (2013), Functional specialization of duplicated AP3-like genes in Medicago truncatula. Plant J, 73: 663–675 , which has been published in final form at http://dx.doi.org/10.1111/tpj.12068 es_ES
dc.description.abstract The Bclass of MADS box genes has been studied in a wide range of plant species, but has remained largely uncharacterized in legumes. Here we investigate the evolutionary fate of the duplicated AP3-like genes of a legume species. To obtain insight into the extent to which B-class MADS box gene functions are conserved or have diversified in legumes, we isolated and characterized the two members of the AP3 lineage in Medicago truncatula: MtNMH7 and MtTM6 (euAP3 and paleoAP3 genes, respectively). A non-overlapping and complementary expression pattern of both genes was observed in petals and stamens. MtTM6 was expressed predominantly in the outer cell layers of both floral organs, and MtNMH7 in the inner cell layers of petals and stamens. Functional analyses by reverse genetics approaches (RNAi and Tnt1 mutagenesis) showed that the contribution of MtNMH7 to petal identity is more important than that of MtTM6, whereas MtTM6 plays a more important role in stamen identity than its paralog MtNMH7. Our results suggest that the M.truncatula AP3-like genes have undergone a functional specialization process associated with complete partitioning of gene expression patterns of the ancestral gene lineage. We provide information regarding the similarities and differences in petal and stamen development among core eudicots. es_ES
dc.description.sponsorship This work was funded by grants BIO2006-09374 and BIO2009-08134 from the Spanish Ministry of Science and Innovation. We are gratefully to Mario A. Fares and Santiago F. Elena (Instituto de Biologia Molecular y Celular de Plantas, Valencia, Spain) for helpful comments and bioinformatics support. The collaboration and assistance of Rafael Martinez-Pardo in the greenhouse is gratefully acknowledged. en_EN
dc.language Inglés es_ES
dc.publisher Wiley-Blackwell es_ES
dc.relation Spanish Ministry of Science and Innovation [BIO2006-09374, BIO2009-08134] es_ES
dc.relation.ispartof The Plant Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject AP3-like genes es_ES
dc.subject MADS box es_ES
dc.subject Legumes es_ES
dc.subject Medicago truncatula es_ES
dc.subject Gene duplication es_ES
dc.subject Sub-functionalization es_ES
dc.subject Evolutionary fate es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Functional specialization of duplicated AP3-like genes in Medicago truncatula es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/tpj.12068
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Roque Mesa, EM.; Serwatowska, J.; Rochina Peñalver, MC.; Wen, J.; Mysore, KS.; Yenush, L.; Beltran Porter, JP.... (2013). Functional specialization of duplicated AP3-like genes in Medicago truncatula. The Plant Journal. 73(4):663-675. doi:10.1111/tpj.12068 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1111/tpj.12068 es_ES
dc.description.upvformatpinicio 663 es_ES
dc.description.upvformatpfin 675 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 73 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 254064 es_ES
dc.identifier.eissn 1365-313X


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