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A cytochrome P450 regulates a domestication trait in cultivated tomato

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A cytochrome P450 regulates a domestication trait in cultivated tomato

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dc.contributor.author Chakrabarti, Manohar es_ES
dc.contributor.author Zhang, Na es_ES
dc.contributor.author Sauvage, Christopher es_ES
dc.contributor.author Muños, Stéphane es_ES
dc.contributor.author Blanca Postigo, José Miguel es_ES
dc.contributor.author Cañizares Sales, Joaquín es_ES
dc.contributor.author Díez Niclós, Mª José Teresa de Jesús es_ES
dc.contributor.author Schneider, Rhiannon es_ES
dc.contributor.author Mazourek, Michael es_ES
dc.contributor.author McClead, Jammi es_ES
dc.contributor.author Causse, Mathilde es_ES
dc.contributor.author Van Der Knaap, Esther Klazina Maria es_ES
dc.date.accessioned 2016-04-14T12:09:13Z
dc.date.available 2016-04-14T12:09:13Z
dc.date.issued 2013-10-15
dc.identifier.issn 0027-8424
dc.identifier.uri http://hdl.handle.net/10251/62559
dc.description.abstract Domestication of crop plants had effects on human lifestyle and agriculture. However, little is known about the underlying molecular mechanisms accompanying the changes in fruit appearance as a consequence of selection by early farmers. We report the fine mapping and cloning of a tomato (Solanum lycopersicum) fruit mass gene encoding the ortholog of KLUH, SlKLUH, a P450 enzyme of the CYP78A subfamily. The increase in fruit mass is predominantly the result of enlarged pericarp and septum tissues caused by increased cell number in the large fruited lines. SlKLUH also modulates plant architecture by regulating number and length of the side shoots, and ripening time, and these effects are particularly strong in plants that transgenically down-regulate SlKLUH expression carrying fruits of a dramatically reduced mass. Association mapping followed by segregation analyses revealed that a single nucleotide polymorphism in the promoter of the gene is highly associated with fruit mass. This single polymorphism may potentially underlie a regulatory mutation resulting in increased SlKLUH expression concomitant with increased fruit mass. Our findings suggest that the allele giving rise to large fruit arose in the early domesticates of tomato and becoming progressively more abundant upon further selections. We also detected association of fruit weight with CaKLUH in chile pepper (Capsicum annuum) suggesting that selection of the orthologous gene may have occurred independently in a separate domestication event. Altogether, our findings shed light on the molecular basis of fruit mass, a key domestication trait in tomato and other fruit and vegetable crops. es_ES
dc.description.sponsorship We thank Dr. D. Choi (Seoul National University) for providing DNA sequence of chile pepper CaKLUH region; and Molecular and Cellular Imaging Center, The Ohio State University, for assistance with microscopy. This work was supported by National Science Foundation Grant IOS-0922661 (to E.v.d.K.). en_EN
dc.language Inglés es_ES
dc.publisher National Academy of Sciences es_ES
dc.relation National Science Foundation/IOS-0922661 es_ES
dc.relation.ispartof Proceedings of the National Academy of Sciences es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Fruit size es_ES
dc.subject Maternal control es_ES
dc.subject Shape variation es_ES
dc.subject Gene family es_ES
dc.subject Organ size es_ES
dc.subject Seed size es_ES
dc.subject Loci es_ES
dc.subject Plants es_ES
dc.subject Expression es_ES
dc.subject Variants es_ES
dc.subject.classification GENETICA es_ES
dc.title A cytochrome P450 regulates a domestication trait in cultivated tomato es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1073/pnas.1307313110
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Chakrabarti, M.; Zhang, N.; Sauvage, C.; Muños, S.; Blanca Postigo, JM.; Cañizares Sales, J.; Díez Niclós, MJTDJ.... (2013). A cytochrome P450 regulates a domestication trait in cultivated tomato. Proceedings of the National Academy of Sciences. 110(42):17125-17130. doi:10.1073/pnas.1307313110 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1073/pnas.1307313110 es_ES
dc.description.upvformatpinicio 17125 es_ES
dc.description.upvformatpfin 17130 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 110 es_ES
dc.description.issue 42 es_ES
dc.relation.senia 252883 es_ES
dc.identifier.eissn 1091-6490
dc.identifier.pmid 24082112 es_ES
dc.identifier.pmcid PMC3801035 es_ES


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